Phalloidin Conjugates
XFD647, produced by AAT Bioquest, shares the identical chemical structure as Alexa Fluor® 647 (Alexa Fluor® being the trademark of ThermoFisher). The XFD647 phalloidin conjugate is essentially equivalent to Alexa Fluor® 647 phalloidin and exhibits a far-red fluorescent signal. This conjugate specifically binds to F-actins, and when used at nanomolar concentrations, it serves as a convenient tool for labeling, identifying, and quantifying F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures, or cell-free experiments. The application of fluorescent phalloidin derivatives, such as XFD647, has proven crucial in the detailed examination of actin networks at high resolution. AAT Bioquest provides a range of fluorescent phalloidin derivatives in different colors to facilitate multicolor imaging applications. They are fully compatible with other fluorescent stains used in cellular analyses, including fluorescent proteins, Qdot™ nanocrystals, and other iFluor® conjugates, including secondary antibodies.
Spectrum
Alternative formats
Name | Conjugate |
Phalloidin-iFluor® 488 Conjugate | iFluor 488 |
Phalloidin-iFluor® 647 Conjugate | iFluor 647 |
Phalloidin-iFluor® 594 Conjugate | iFluor 594 |
Phalloidin-iFluor® 555 Conjugate | iFluor 555 |
Phalloidin-iFluor® 633 Conjugate | iFluor 633 |
Phalloidin-iFluor® 532 Conjugate | iFluor 532 |
Phalloidin-iFluor® 405 Conjugate | iFluor 405 |
Phalloidin-iFluor® 514 Conjugate | iFluor 514 |
Phalloidin-iFluor® 350 Conjugate | iFluor 350 |
Show More (14) |
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (280 nm) |
XFD350 Phalloidin *XFD350 Same Structure to Alexa Fluor™ 350* | 343 | 441 | 19000 | - | 0.19 |
XFD488 Phalloidin *XFD488 Same Structure to Alexa Fluor™ 488* | 499 | 520 | 73000 | 0.921 | 0.11 |
XFD594 Phalloidin *XFD594 Same Structure to Alexa Fluor™ 594* | 590 | 618 | 92000 | 0.661 | 0.56 |
XFD647 Azide | 650 | 671 | 270000 | 0.331 | 0.03 |
XFD647 Alkyne | 650 | 671 | 270000 | 0.331 | 0.03 |
XFD647 amine | 650 | 671 | 270000 | 0.331 | 0.03 |
XFD647 acid | 650 | 671 | 270000 | 0.331 | 0.03 |
References
Authors: Montecchi-Palmer, Michela and Bermudez, Jaclyn Y and Webber, Hannah C and Patel, Gaurang C and Clark, Abbot F and Mao, Weiming
Journal: Investigative ophthalmology & visual science (2017): 1288-1295
Authors: Muñiz, Javier and Romero, Juan and Holubiec, Mariana and Barreto, George and González, Janneth and Saint-Martin, Madeleine and Blanco, Eduardo and Carlos Cavicchia, Juan and Castilla, Rocío and Capani, Francisco
Journal: Brain research (2014): 81-90
Authors: Haucke, Elisa and Navarrete-Santos, Alexander and Simm, Andreas and Silber, Rolf-Edgar and Hofmann, Britt
Journal: Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society (2014): 239-45
Authors: Saraceno, G E and Ayala, M V and Badorrey, M S and Holubiec, M and Romero, J I and Galeano, P and Barreto, G and Giraldez-Alvárez, L D and Kölliker-Fres, R and Coirini, H and Capani, F
Journal: Synapse (New York, N.Y.) (2012): 9-19
Authors: Job, Raly and Raja, Vignesh and Grierson, Ian and Currie, Laura and O'Reilly, Steven and Pollock, Natalie and Knight, Emma and Clark, Abbot F
Journal: The British journal of ophthalmology (2010): 1388-92