logo
AAT Bioquest

Phalloidin Conjugates

equivalent to Alexa Fluor® 647 phalloidin

XFD647, produced by AAT Bioquest, shares the identical chemical structure as Alexa Fluor® 647 (Alexa Fluor® being the trademark of ThermoFisher). The XFD647 phalloidin conjugate is essentially equivalent to Alexa Fluor® 647 phalloidin and exhibits a far-red fluorescent signal. This conjugate specifically binds to F-actins, and when used at nanomolar concentrations, it serves as a convenient tool for labeling, identifying, and quantifying F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures, or cell-free experiments. The application of fluorescent phalloidin derivatives, such as XFD647, has proven crucial in the detailed examination of actin networks at high resolution. AAT Bioquest provides a range of fluorescent phalloidin derivatives in different colors to facilitate multicolor imaging applications. They are fully compatible with other fluorescent stains used in cellular analyses, including fluorescent proteins, Qdot™ nanocrystals, and other iFluor® conjugates, including secondary antibodies.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (280 nm)
XFD350 Phalloidin *XFD350 Same Structure to Alexa Fluor™ 350*34344119000-0.19
XFD488 Phalloidin *XFD488 Same Structure to Alexa Fluor™ 488*499520730000.9210.11
XFD594 Phalloidin *XFD594 Same Structure to Alexa Fluor™ 594*590618920000.6610.56
XFD647 Azide6506712700000.3310.03
XFD647 Alkyne6506712700000.3310.03
XFD647 amine6506712700000.3310.03
XFD647 acid6506712700000.3310.03

References

View all 10 references: Citation Explorer
TGFβ2 Induces the Formation of Cross-Linked Actin Networks (CLANs) in Human Trabecular Meshwork Cells Through the Smad and Non-Smad Dependent Pathways.
Authors: Montecchi-Palmer, Michela and Bermudez, Jaclyn Y and Webber, Hannah C and Patel, Gaurang C and Clark, Abbot F and Mao, Weiming
Journal: Investigative ophthalmology & visual science (2017): 1288-1295
Neuroprotective effects of hypothermia on synaptic actin cytoskeletal changes induced by perinatal asphyxia.
Authors: Muñiz, Javier and Romero, Juan and Holubiec, Mariana and Barreto, George and González, Janneth and Saint-Martin, Madeleine and Blanco, Eduardo and Carlos Cavicchia, Juan and Castilla, Rocío and Capani, Francisco
Journal: Brain research (2014): 81-90
Glycation of extracellular matrix proteins impairs migration of immune cells.
Authors: Haucke, Elisa and Navarrete-Santos, Alexander and Simm, Andreas and Silber, Rolf-Edgar and Hofmann, Britt
Journal: Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society (2014): 239-45
Effects of perinatal asphyxia on rat striatal cytoskeleton.
Authors: Saraceno, G E and Ayala, M V and Badorrey, M S and Holubiec, M and Romero, J I and Galeano, P and Barreto, G and Giraldez-Alvárez, L D and Kölliker-Fres, R and Coirini, H and Capani, F
Journal: Synapse (New York, N.Y.) (2012): 9-19
Cross-linked actin networks (CLANs) are present in lamina cribrosa cells.
Authors: Job, Raly and Raja, Vignesh and Grierson, Ian and Currie, Laura and O'Reilly, Steven and Pollock, Natalie and Knight, Emma and Clark, Abbot F
Journal: The British journal of ophthalmology (2010): 1388-92
Page updated on October 9, 2024

Ordering information

Price
Conjugate
Unit size
Catalog Number
231002310123102231032311023111231152311623117231192312223125231272312823129231302313123140231502315323158231595301
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Physical properties

Molecular weight

1408.65

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.00

Correction Factor (280 nm)

0.03

Extinction coefficient (cm -1 M -1)

239000

Excitation (nm)

650

Emission (nm)

671

Quantum yield

0.331

Storage, safety and handling

H-phraseH301, H311, H331
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR23, R24, R25

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200