XFD647 phalloidin is a high-affinity probe for filamentous actin (F-actin), conjugated to XFD647, a bright, photostable, far-red fluorescent dye. XFD647, manufactured by AAT Bioquest, is structurally similar to Alexa Fluor™ 647 (Thermo Fisher), ensuring comparable performance in fluorescence applications.

Phalloidin, a bicyclic peptide toxin derived from Amanita phalloides (commonly known as the death cap mushroom), is well known for its high specificity and strong binding affinity to F-actin. When conjugated to XFD647, phalloidin enables precise, high-contrast visualization of F-actin structures with minimal background interference. This fluorescent conjugate is highly effective for imaging F-actin in a wide range of biological specimens, including plant and animal tissues, fixed and permeabilized cells, and cell-free systems.

XFD647 phalloidin binds to F-actin with nanomolar affinity, making it an excellent tool for labeling, identifying, and quantifying actin filaments. Its superior photostability and blinking properties make it particularly well suited for super-resolution microscopy techniques such as SIM and STORM. Additionally, XFD647 phalloidin staining is fully compatible with other fluorescent labels, including fluorescent proteins, Qdot nanocrystals, and iFluor®-conjugated secondary antibodies, allowing seamless integration into multiplex fluorescence imaging workflows.