Phalloidin Conjugates | AAT Bioquest

Phalloidin-iFluor® 594 Conjugate

Phalloidin-iFluor® 594 Conjugate is a red-fluorescent probe designed for selective detection and visualization of filamentous actin (F-actin) in fixed and permeabilized cells and tissues.

Binding Specificity: Selectively binds F-actin with high affinity.
Spectral Properties: Excitation/emission maxima of 587/603 nm.
Instrument Compatibility: Compatible with TRITC and Texas Red fluorescence microscope filter sets.
Applications: Suitable for actin cytoskeleton staining and multicolor fluorescence microscopy.

<p>Fluorescence image of HeLa cells fixed with 4% formaldehyde then stained with Cell Navigator® F-Actin Labeling Kit *Red Fluorescence* in a Costar black 96-well plate. Cells were labeled with Phalloidin-iFluor® 594  (Cat#23122, Red) and nuclei stain DAPI (Cat#17507, Blue), respectively. Cell endoplasmic reticulum (ER) was stained with ER Green™ (Cat#22635, Green) before fixation.</p>

Protocol

SDS

Catalog	Size	Price	Quantity
23122	300 Tests	Price

Overview

This red fluorescent phalloidin conjugate (equivalent to Alexa Fluor® 594-labeled phalloidin) selectively binds to F-actins. Used at nanomolar concentrations, phalloidin derivatives are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. Phalloidin binds to actin filaments much more tightly than to actin monomers, leading to a decrease in the rate constant for the dissociation of actin subunits from filament ends, essentially stabilizing actin filaments through the prevention of filament depolymerization. Moreover, phalloidin is found to inhibit the ATP hydrolysis activity of F-actin. Phalloidin functions differently at various concentrations in cells. When introduced into the cytoplasm at low concentrations, phalloidin recruits the less polymerized forms of cytoplasmic actin as well as filamin into stable "islands" of aggregated actin polymers, yet it does not interfere with stress fibers, i.e. thick bundles of microfilaments. The property of phalloidin is a useful tool for investigating the distribution of F-actin in cells by labeling phalloidin with fluorescent analogs and using them to stain actin filaments for light microscopy. Fluorescent derivatives of phalloidin have turned out to be enormously useful in localizing actin filaments in living or fixed cells as well as for visualizing individual actin filaments in vitro. Fluorescent phalloidin derivatives have been used as an important tool in the study of actin networks at high resolution. AAT Bioquest offers a variety of fluorescent phalloidin derivatives with different colors for multicolor imaging applications.

Citations

View all 179 citations: Citation Explorer

The effect of macrophage secretome on equine endometrial fibroblast transcriptomic and functional characteristics

Authors:

S{\l}yszewska, Magda and Sadowska, Agnieszka and Tobolski, Dawid and Piotrowska-Tomala, Katarzyna and Ferreira-Dias, Gra{\c{c}}a and Sz{\'o}stek-Mioduchowska, Anna

Journal:

Theriogenology (2026): 118177

Silencing LIMK1 Can Inhibit ROS-mediated NF-$\kappa$B/NLRP3 Pathway Activation and Reduce Inflammatory Injury in H9c2 Cardiomyocytes

Authors:

Huang, Zhijian and Zeng, Youxiao and Huang, Xue’e and Huang, Lihong and He, Yafen and Zhou, Yijun

Journal:

Iranian Journal of Allergy, Asthma and Immunology (2026): 1--20

Cellular Mechanical Phenotyping and Therapeutic Drug Screening in Frozen Shoulder Based on Micropillar Arrays

Authors:

Sun, Jianfeng and Yu, Dengjie and Chen, Changxu and Yang, Juwei and Li, Jiaxuan and Luo, Ruizeng and Li, Longfei and Jin, Shuo and Liu, Zhuo and Li, Zhou and others

Journal:

Nano Energy (2026): 112174

Small nucleolar RNA Snora61 drives self-renewal of intestinal stem cells via initiation of Lgr5 transcription

Authors:

He, Jiacheng and Lan, Yufei and Xu, Yuwei and Xiong, Zhen and Yi, Zhibin and Guo, Hui and Zhang, Jiahang and Zhou, Ziheng and Du, Ying and Pan, Fan and others

Journal:

Nature Communications (2026)

RNA interference screen against SARS-CoV-2 identifies proviral vesicular transport factors involved in release

Authors:

Kerr, Holly EM and Daniels, Alison and Fletcher, Sarah L and Craig, Nicky and O'Shea, Marie and Griffiths, Samantha J and Sachs, Daniel and Donovan, Sophie and Fragkoudis, Rennos and Haas, Juergen G and others

Journal:

Journal of General Virology (2026): 002216

References

View all 127 references: Citation Explorer

Phalloidin perturbs the interaction of human non-muscle myosin isoforms 2A and 2C1 with F-actin

Authors:

Diensthuber RP, Muller M, Heissler SM, Taft MH, Chizhov I, Manstein DJ.

Journal:

FEBS Lett (2011): 767

Improved penile histology by phalloidin stain: circular and longitudinal cavernous smooth muscles, dual-endothelium arteries, and erectile dysfunction-associated changes

Authors:

Lin G, Qiu X, F and el TM, Albersen M, Wang Z, Lue TF, Lin CS.

Journal:

Urology (2011): 970 e1

pH-(low)-insertion-peptide (pHLIP) translocation of membrane impermeable phalloidin toxin inhibits cancer cell proliferation

Authors:

An M, Wijesinghe D, Andreev OA, Reshetnyak YK, Engelman DM.

Journal:

Proc Natl Acad Sci U S A (2010): 20246

Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging

Authors:

Chazotte B.

Journal:

Cold Spring Harb Protoc (2010): pdb prot4947

Protective effect of bile acid derivatives in phalloidin-induced rat liver toxicity

Authors:

Herraez E, Macias RI, Vazquez-Tato J, Hierro C, Monte MJ, Marin JJ.

Journal:

Toxicol Appl Pharmacol (2009): 21

Physical properties

Molecular weight	~1600
Solvent	DMSO

Spectral properties

Absorbance (nm)	587
Correction factor (260 nm)	0.05
Correction factor (280 nm)	0.04
Extinction coefficient (cm ^-1 M ^-1)	200000 ¹
Excitation (nm)	587
Emission (nm)	603
Quantum yield	0.53 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 2, 2026