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XFD488 Phalloidin
XFD488 is manufactured by AAT Bioquest, and it has a chemical structure similar to that of Alexa Fluor® 488 (Alexa Fluor® is the trademark of Thermo Fisher). XFD488 phalloidin conjugate is chemically equivalent to Alexa Fluor® 488 phalloidin. This green fluorescent phalloidin conjugate selectively binds to F-actins. Used at nanomolar concentrations, phalloidin derivatives are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. Fluorescent phalloidin derivatives have been used as an important tool in the study of actin networks at high resolution. AAT Bioquest offers a variety of fluorescent phalloidin derivatives with different colors for multicolor imaging applications.
<strong>Fixed and stained HeLa cells.</strong><br>HeLa cells were fixed with 4% formaldehyde, permeabilized, and blocked. F-actin were stained with XFD488 phalloidin (Cat No. 23153) and nuclei labeled with Nuclear Red&trade; DCS1 (Cat No. 17552). Images were acquired on a Keyence BZ-X710 all-in-one fluorescence microscope.
<strong>Fixed and stained HeLa cells.</strong><br>HeLa cells were fixed with 4% formaldehyde, permeabilized, and blocked. F-actin were stained with XFD488 phalloidin (Cat No. 23153) and nuclei labeled with Nuclear Red&trade; DCS1 (Cat No. 17552). Images were acquired on a Keyence BZ-X710 all-in-one fluorescence microscope.
CatalogSize
Price
Quantity
23153300 Tests
Price
 
Physical properties

Molecular weight~1300
SolventDMSO
Spectral properties

Correction factor (260 nm)0.3
Correction factor (280 nm)0.11
Extinction coefficient (cm -1 M -1)
73000
Excitation (nm)499
Emission (nm)520
Quantum yield
0.92
1
Storage, safety and handling

H-phraseH301, H311, H331
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR23, R24, R25
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings

Fluorescence microscope
ExcitationFITC filter
EmissionFITC filter
Recommended plateBlack wall/clear bottom
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Page updated on August 11, 2025