Name: iFluor® 790 Styramide™
Brand: AAT Bioquest
SKU: 45070
Price: 355 USD
Availability: InStock

iFluor® 790 Styramide™

iFluor® 790 Styramide™ is a near-infrared fluorescent signal amplification reagent designed for sensitive detection of low-abundance targets in cells and tissues using HRP-mediated labeling.

Detection Principle: Uses HRP-catalyzed covalent deposition of fluorescent labels.
High Sensitivity: Generates 10–50 times stronger fluorescence signals than conventional tyramide reagents.
Spectral Properties: Excitation/emission maxima of 787/812 nm.
Instrument Compatibility: Compatible with Cy7 fluorescence microscope filter sets.
Applications: Suitable for immunocytochemistry, immunofluorescence, and immunohistochemistry.

Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ.  PSA radicals have much higher reactivity than tyramide radicals, making the PSA system much faster, more robust and sensitive than the traditional TSA reagents.

Protocol

SDS

Catalog	Size	Price	Quantity
45070	100 Slides	Price

Overview

Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA™ utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA™ radicals have much higher reactivity than tyramide radicals, making the PSA™ system much faster, more robust and sensitive than the traditional TSA reagents. Compared to tyramide reagents, the Styramide™ conjugates have ability to label the target at higher efficiency and thus generate significantly higher fluorescence signal. Styramide™ conjugates also allow significantly less consumption of primary antibody compared to standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 790 Styramide is a superior replacement for Alexa Fluor 790 tyramide or other spectrally similar fluorescent tyramide conjugates or TSA reagents.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 790 Styramide™ to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	51.293 µL	256.464 µL	512.928 µL	2.565 mL	5.129 mL
5 mM	10.259 µL	51.293 µL	102.586 µL	512.928 µL	1.026 mL
10 mM	5.129 µL	25.646 µL	51.293 µL	256.464 µL	512.928 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1949.59 g/mol	=	mL	x	mM	=	mmol

Physical properties

Molecular weight	1949.59
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.1
Correction factor (280 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	250000 ¹
Excitation (nm)	787
Emission (nm)	812
Quantum yield	0.13 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Fluorescence microscope
Excitation	Cy7 filter set
Emission	Cy7 filter set
Recommended plate	Black wall/clear bottom

Telephone
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Email	sales@aatbio.com
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Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 30, 2026