Name: iFluor® 594 Styramide™
Brand: AAT Bioquest
SKU: 45035
Price: 355 USD
Availability: InStock

iFluor® 594 Styramide™

iFluor® 594 Styramide™ is a fluorescent signal amplification reagent designed for highly sensitive detection of low-abundance targets in cells and tissues through HRP-mediated labeling.

Detection Principle: Uses HRP-catalyzed covalent deposition of fluorescent labels.
High Sensitivity: Generates 10–50 times stronger fluorescence signals than conventional tyramide reagents.
Enhanced Fluorescence: Features bright, photostable iFluor® 594 dye.
Spectral Properties: Excitation/emission maxima of 587/603 nm.
Applications: Suitable for immunocytochemistry, immunofluorescence, and immunohistochemistry.

Formalin-fixed, paraffin-embedded (FFPE) human lung tissue was labeled with anti-EpCAM mouse mAb followed by HRP-labeled goat anti-mouse IgG (Cat No. 16728). The fluorescence signal was developed using iFluor® 594 styramide (Cat No. 45035) and detected with a TRITC/Cy3 filter set. Nuclei (blue) were counterstained with DAPI (Cat No. 17507).

Protocol

SDS

Catalog	Size	Price	Quantity
45035	100 Slides	Price

Overview

Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA™ utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA™ radicals have much higher reactivity than tyramide radicals, making the PSA™ system much faster, more robust and sensitive than the traditional TSA reagents. Compared to tyramide reagents, the Styramide™ conjugates have ability to label the target at higher efficiency and thus generate significantly higher fluorescence signal. Styramide™ conjugates also allow significantly less consumption of primary antibody compared to standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 594 Styramide is a superior replacement for Alexa Fluor 594 tyramide or other spectrally similar fluorescent tyramide conjugates or TSA reagents.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 594 Styramide™ to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	74.532 µL	372.659 µL	745.318 µL	3.727 mL	7.453 mL
5 mM	14.906 µL	74.532 µL	149.064 µL	745.318 µL	1.491 mL
10 mM	7.453 µL	37.266 µL	74.532 µL	372.659 µL	745.318 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1341.71 g/mol	=	mL	x	mM	=	mmol

Citations

View all 6 citations: Citation Explorer

A cross-species atlas of the dorsal vagal complex reveals neural mediators of the effects of cagrilintide on energy balance

Authors:

Ludwig, Mette Q and Coester, Bernd and Gordian, Desiree and Hassan, Shad and Tomlinson, Abigail J and Toure, Mouhamadoul Habib and Christensen, Oliver P and Lommi, Greta and Moltke-Prehn, Anja and Brown, Jenny M and others,

Journal:

Nature Metabolism (2026): 1--18

Tyramide signal amplification-based detection system: A novel approach to improve detection efficiency for circulating tumor cells

Authors:

Chen, Lin and Yang, Zhonglin and Lu, Yue and Li, Shan and Tang, Dongjiang and Zhang, Lei

Journal:

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy (2025): 126898

Hypothalamic PNOC/NPY neurons constitute mediators of leptin-controlled energy homeostasis

Authors:

Solheim, Marie H and Stroganov, Sima and Chen, Weiyi and Subagia, P Sicilia and Bauder, Corinna A and Wnuk-Lipinski, Daria and Del R{\'\i}o-Mart{\'\i}n, Almudena and Sotelo-Hitschfeld, Tamara and Beddows, Cait A and Klemm, Paul and others,

Journal:

Cell (2025)

A Cross-Species Atlas of the Dorsal Vagal Complex Reveals Neural Mediators of Cagrilintide′ s Effects on Energy Balance

Authors:

Ludwig, Mette Q and Coester, Bernd and Gordian, Desiree and Hassan, Shad and Tomlinson, Abigail J and Toure, Mouhamadoul Habib and Christensen, Oliver P and Moltke-Prehn, Anja and Brown, Jenny M and Rausch, Dylan M and others,

Journal:

bioRxiv (2025): 2025--01

Membrane progesterone receptor $\gamma$ (paqr5b) is essential for the formation of neurons in the zebrafish olfactory rosette

Authors:

Mustary, Umme Habiba and Maeno, Akiteru and Rahaman, Md Mostafizur and Ali, Md Hasan and Tokumoto, Toshinobu

Journal:

Scientific Reports (2024): 24354

Physical properties

Molecular weight	1341.71
Solvent	DMSO

Spectral properties

Absorbance (nm)	587
Correction factor (260 nm)	0.05
Correction factor (280 nm)	0.04
Extinction coefficient (cm ^-1 M ^-1)	200000 ¹
Excitation (nm)	587
Emission (nm)	603
Quantum yield	0.53 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Fluorescence microscope
Excitation	Cy3/TRITC filter set
Emission	Cy3/TRITC filter set
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Compatible with Texas Red filter set

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 30, 2026