iFluor® 840 succinimidyl ester

iFluor® 840 succinimidyl ester is an amine-reactive, near-infrared fluorescent dye designed for covalent labeling of proteins, particularly antibodies, for fluorescence imaging and detection applications.

Amine-Reactive Labeling: Covalently conjugates to primary amino groups of proteins.
Spectral Properties: Excitation/emission maxima of 836/879 nm.
Spectral Compatibility: Infrared emission is well separated from Cy5, Cy7, and APC fluorophores.
Applications: Suitable for small-animal in vivo imaging and multicolor fluorescence analysis.

Fluorescent dye NHS esters (or succinimidyl esters) are the most popular tool for conjugating dyes to a peptide, protein, antibody, amino-modified oligonucleotide or nucleic acid. NHS esters react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.

Protocol

Catalog	Size	Price
1403	1 mg	Price
71403	100 ug	Price
71528	5 mg	Price
71578	10 mg	Price

Overview

In vivo fluorescence imaging uses a sensitive camera to detect the fluorescence emission from fluorophores in whole-body living small animals. To overcome the photon attenuation in living tissue, fluorophores with long emission at the infrared (IR) region are generally preferred. Recent advances in imaging strategies and reporter techniques for in vivo fluorescence imaging include novel approaches to improve the specificity and affinity of the probes and to modulate and amplify the signal at target sites for enhanced sensitivity. Further emerging developments aim to achieve high-resolution, multimodality, and lifetime-based in vivo fluorescence imaging. Our iFluor® 840 is designed to label proteins and other biomolecules with infrared fluorescence. Conjugates prepared with iFluor® 840 have excitation and emission in the IR range. iFluor® 840 dye emission is well separated from commonly used far-red fluorophores such as Cy5, Cy7, or allophycocyanin (APC), facilitating multicolor analysis. This fluorophore is also useful for small animal in vivo imaging applications or other imaging applications requiring IR detection. iFluor® 840 succinimidyl ester is amine-reactive and can be readily used to conjugate amine-containing biomolecules, particularly antibodies.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 840 succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	70.323 µL	351.617 µL	703.235 µL	3.516 mL	7.032 mL
5 mM	14.065 µL	70.323 µL	140.647 µL	703.235 µL	1.406 mL
10 mM	7.032 µL	35.162 µL	70.323 µL	351.617 µL	703.235 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1422 g/mol	=	mL	x	mM	=	mmol

Citations

View all 10 citations: Citation Explorer

A SARS-CoV-2 vaccine on an NIR-II/SWIR emitting nanoparticle platform

Authors:

Jiang, Yingying and Sanyal, Mrinmoy and Hussein, Noor A and Baghdasaryan, Ani and Zhang, Mengzhen and Wang, Feifei and Ren, Fuqiang and Li, Jiachen and Zhu, Guanzhou and Meng, Yifan and others

Journal:

Science Advances (2025): eadp5539

High-dose Ascorbate Exhibits Anti-proliferative and Anti-invasive Effects Dependent on PTEN/AKT/mTOR Pathway in Endometrial Cancer in vitro and in vivo

Authors:

Shen, Xiaochang and Wang, Jiandong and Kong, Weimin and John, Catherine and Deng, Boer and Chen, Shuning and Zhang, Haomeng and Haag, Jennifer and Sinha, Nikita and Sun, Wenchuan and others

Journal:

International Journal of Biological Sciences (2025): 1545--1565

Block Face Scanning Electron Microscopy of Fluorescently Labeled Axons Without Using Near Infra-Red Branding

Authors:

Maclachlan, C., Sahlender, D. A., Hayashi, S., Molnar, Z., Knott, G.

Journal:

Front Neuroanat (2018): 88

Bioactive magnetic near Infra-Red fluorescent core-shell iron oxide/human serum albumin nanoparticles for controlled release of growth factors for augmentation of human mesenchymal stem cell growth and differentiation

Authors:

Levy, I., Sher, I., Corem-Salkmon, E., Ziv-Polat, O., Meir, A., Treves, A. J., Nagler, A., Kalter-Leibovici, O., Margel, S., Rotenstreich, Y.

Journal:

J Nanobiotechnology (2015): 34

Novel near infra-red fluorescent pH sensors based on 1-aminoperylene bisimides covalently grafted onto poly(acryloylmorpholine)

Authors:

Aigner, D., Borisov, S. M., Petritsch, P., Klimant, I.

Journal:

Chem Commun (Camb) (2013): 2139-41

References

View all 19 references: Citation Explorer

Nanovesicle delivery to the liver via retinol binding protein and platelet-derived growth factor receptors: how targeting ligands affect biodistribution

Authors:

Hsu, Ching-Yun and Chen, Chun-Han and Aljuffali, Ibrahim A and Dai, You-Shan and Fang, Jia-You

Journal:

Nanomedicine (2017)

In vivo monitoring the fate of Cy5.5-Tat labeled T lymphocytes by quantitative near-infrared fluorescence imaging during acute brain inflammation in a rat model of experimental autoimmune encephalomyelitis

Authors:

Berger C, Gremlich HU, Schmidt P, Cannet C, Kneuer R, Hiest and P, Rausch M, Rudin M.

Journal:

J Immunol Methods (2007): 65

A target cell-specific activatable fluorescence probe for in vivo molecular imaging of cancer based on a self-quenched avidin-rhodamine conjugate

Authors:

Hama Y, Urano Y, Koyama Y, Kamiya M, Bernardo M, Paik RS, Shin IS, Paik CH, Choyke PL, Kobayashi H.

Journal:

Cancer Res (2007): 2791

Fluorescence imaging in vivo: recent advances

Authors:

Rao J, Dragulescu-Andrasi A, Yao H.

Journal:

Curr Opin Biotechnol (2007): 17

Ex vivo fluorescence imaging of normal and malignant urothelial cells to enhance early diagnosis

Authors:

Steenkeste K, Lecart S, Deniset A, Pernot P, Eschwege P, Ferlicot S, Leveque-Fort S, Bri and et R, Fontaine-Aupart MP.

Journal:

Photochem Photobiol (2007): 1157

Physical properties

Molecular weight	1422
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.2
Correction factor (280 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	200000 ¹
Excitation (nm)	836
Emission (nm)	879

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on November 9, 2026