Name: iFluor® 510 maleimide
Brand: AAT Bioquest
SKU: 1425
Price: 237 USD
Availability: InStock

iFluor® 510 maleimide

iFluor® 510 Maleimide is a green-fluorescent, thiol-reactive dye for selective covalent labeling of antibodies, proteins, and other thiol-containing biomolecules in fluorescence-based research applications.

Thiol-Reactive Functionality: Maleimide group selectively reacts with thiols, including cysteine residues.
Spectral Properties: Excitation at 511 nm and emission at 530 nm.
Fluorescence Performance: Bright and photostable fluorescence.
Instrument Compatibility: Supports blue-green excitation in the 488–514 nm range.
Applications: Suitable for bioconjugation for fluorescence microscopy, flow cytometry, and other fluorescence-based assays.

Fluorescent dye maleimides are the most popular tool for conjugating dyes to a peptide, protein, antibody, thiol-modified oligonucleotide or nucleic acid through their SH group. Maleimides react readily with the thiol group of proteins, thiol-modified oligonucleotides, and other thiol-containing molecules under neutral conditions. The resulting dye conjugates are quite stable.

Protocol

SDS

Catalog	Size	Price	Quantity
1425	1 mg	Price

Overview

iFluor® 510 maleimide selectively reacts with thiol group of a biomolecule. It is widely used to label the reduced antibodies. iFluor®510 is a new fluorescent dye belonging to the iFluor® family of dyes. These dyes are known for their bright fluorescence, photostability, and compatibility with various imaging techniques and instruments. iFluor® 510 emits green fluorescence when excited with light in the blue to green range (around 488 to 514 nm). Its emission peak is typically around 520 to 530 nm. The number "510" in its name represents the approximate maximum excitation wavelength. Like other iFluor® dyes, AAT Bioquest offers a variety of iFluor® 510 derivatives that can be readily used to develop conjugates for biological research and imaging applications. They can be conjugated to various biomolecules, such as antibodies, proteins, nucleic acids, and small molecules, for fluorescence microscopy, flow cytometry, immunohistochemistry, and other fluorescence-based assays.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 510 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	114.185 µL	570.926 µL	1.142 mL	5.709 mL	11.419 mL
5 mM	22.837 µL	114.185 µL	228.37 µL	1.142 mL	2.284 mL
10 mM	11.419 µL	57.093 µL	114.185 µL	570.926 µL	1.142 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	875.77 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

Recent Advances in N-Heterocyclic Small Molecules for Synthesis and Application in Direct Fluorescence Cell Imaging.

Authors:

Li, Yanan and Liu, Tao and Sun, Jianan

Journal:

Molecules (Basel, Switzerland) (2023)

Activatable Nanoprobe with Aggregation-Induced Dual Fluorescence and Photoacoustic Signal Enhancement for Tumor Precision Imaging and Radiotherapy.

Authors:

Yuan, Meng and Fang, Xiao and Wu, Ying and Xu, Yuanji and Feng, Hongjuan and Mu, Jing and Chen, Zhongxiang and Lin, Yuhong and Fu, Qinrui and Du, Wei and Yang, Huanghao and Song, Jibin

Journal:

Analytical chemistry (2022): 5204-5211

Labeling of Proteins for Single-Molecule Fluorescence Spectroscopy.

Authors:

Zosel, Franziska and Holla, Andrea and Schuler, Benjamin

Journal:

Methods in molecular biology (Clifton, N.J.) (2022): 207-233

Design and Validation of Site-Specifically Labeled Single-Domain Antibody-Based Tracers for in Vivo Fluorescence Imaging and Image-Guided Surgery.

Authors:

Declerck, Noemi B and Mateusiak, Lukasz and Hernot, Sophie

Journal:

Methods in molecular biology (Clifton, N.J.) (2022): 395-407

Sensitive and specific detection of breast cancer lymph node metastasis through dual-modality magnetic particle imaging and fluorescence molecular imaging: a preclinical evaluation.

Authors:

Wang, Guorong and Li, Wenzhe and Shi, Guangyuan and Tian, Yu and Kong, Lingyan and Ding, Ning and Lei, Jing and Jin, Zhengyu and Tian, Jie and Du, Yang

Journal:

European journal of nuclear medicine and molecular imaging (2022): 2723-2734

Physical properties

Molecular weight	875.77
Solvent	DMSO

Spectral properties

Excitation (nm)	511
Emission (nm)	530

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 25, 2026