iFluor® 510 maleimide
Ordering information
Price | |
Catalog Number | |
Unit Size | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 875.77 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
iFluor® 510 succinimidyl ester |
Overview | ![]() ![]() |
See also: iFluor® Dyes and Kits
Molecular weight 875.77 |
iFluor® 510 maleimide selectively reacts with thiol group of a biomolecule. It is widely used to label the reduced antibodies. iFluor®510 is a new fluorescent dye belonging to the iFluor® family of dyes. These dyes are known for their bright fluorescence, photostability, and compatibility with various imaging techniques and instruments. iFluor 510 emits green fluorescence when excited with light in the blue to green range (around 488 to 514 nm). Its emission peak is typically around 520 to 530 nm. The number "510" in its name represents the approximate maximum excitation wavelength. Like other iFluor dyes, AAT Bioquest offers a variety of iFluor 510 derivatives that can be readily used to develop conjugates for biological research and imaging applications. They can be conjugated to various biomolecules, such as antibodies, proteins, nucleic acids, and small molecules, for fluorescence microscopy, flow cytometry, immunohistochemistry, and other fluorescence-based assays.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 510 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 114.185 µL | 570.926 µL | 1.142 mL | 5.709 mL | 11.419 mL |
5 mM | 22.837 µL | 114.185 µL | 228.37 µL | 1.142 mL | 2.284 mL |
10 mM | 11.419 µL | 57.093 µL | 114.185 µL | 570.926 µL | 1.142 mL |
Molarity calculator
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Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 350 maleimide | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
iFluor® 488 maleimide | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 555 maleimide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 647 maleimide | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
iFluor® 680 maleimide | 684 | 701 | 2200001 | 0.231 | 0.097 | 0.094 |
iFluor® 700 maleimide | 690 | 713 | 2200001 | 0.231 | 0.09 | 0.04 |
iFluor® 750 maleimide | 757 | 779 | 2750001 | 0.121 | 0.044 | 0.039 |
iFluor® 790 maleimide | 787 | 812 | 2500001 | 0.131 | 0.1 | 0.09 |
iFluor® 800 maleimide | 801 | 820 | 2500001 | 0.111 | 0.03 | 0.08 |
Show More (20) |
References
View all 50 references: Citation Explorer
Recent Advances in N-Heterocyclic Small Molecules for Synthesis and Application in Direct Fluorescence Cell Imaging.
Authors: Li, Yanan and Liu, Tao and Sun, Jianan
Journal: Molecules (Basel, Switzerland) (2023)
Authors: Li, Yanan and Liu, Tao and Sun, Jianan
Journal: Molecules (Basel, Switzerland) (2023)
Sensitive and specific detection of breast cancer lymph node metastasis through dual-modality magnetic particle imaging and fluorescence molecular imaging: a preclinical evaluation.
Authors: Wang, Guorong and Li, Wenzhe and Shi, Guangyuan and Tian, Yu and Kong, Lingyan and Ding, Ning and Lei, Jing and Jin, Zhengyu and Tian, Jie and Du, Yang
Journal: European journal of nuclear medicine and molecular imaging (2022): 2723-2734
Authors: Wang, Guorong and Li, Wenzhe and Shi, Guangyuan and Tian, Yu and Kong, Lingyan and Ding, Ning and Lei, Jing and Jin, Zhengyu and Tian, Jie and Du, Yang
Journal: European journal of nuclear medicine and molecular imaging (2022): 2723-2734
Activatable Nanoprobe with Aggregation-Induced Dual Fluorescence and Photoacoustic Signal Enhancement for Tumor Precision Imaging and Radiotherapy.
Authors: Yuan, Meng and Fang, Xiao and Wu, Ying and Xu, Yuanji and Feng, Hongjuan and Mu, Jing and Chen, Zhongxiang and Lin, Yuhong and Fu, Qinrui and Du, Wei and Yang, Huanghao and Song, Jibin
Journal: Analytical chemistry (2022): 5204-5211
Authors: Yuan, Meng and Fang, Xiao and Wu, Ying and Xu, Yuanji and Feng, Hongjuan and Mu, Jing and Chen, Zhongxiang and Lin, Yuhong and Fu, Qinrui and Du, Wei and Yang, Huanghao and Song, Jibin
Journal: Analytical chemistry (2022): 5204-5211
Labeling of Proteins for Single-Molecule Fluorescence Spectroscopy.
Authors: Zosel, Franziska and Holla, Andrea and Schuler, Benjamin
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 207-233
Authors: Zosel, Franziska and Holla, Andrea and Schuler, Benjamin
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 207-233
Design and Validation of Site-Specifically Labeled Single-Domain Antibody-Based Tracers for in Vivo Fluorescence Imaging and Image-Guided Surgery.
Authors: Declerck, Noemi B and Mateusiak, Lukasz and Hernot, Sophie
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 395-407
Authors: Declerck, Noemi B and Mateusiak, Lukasz and Hernot, Sophie
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 395-407
Quantification of Intracellular Thiols by HPLC-Fluorescence Detection.
Authors: Yamamoto, Hiroki and Fujiwara, Takuya and Funatsu, Takashi and Tsunoda, Makoto
Journal: Molecules (Basel, Switzerland) (2021)
Authors: Yamamoto, Hiroki and Fujiwara, Takuya and Funatsu, Takashi and Tsunoda, Makoto
Journal: Molecules (Basel, Switzerland) (2021)
Turn-on mode diarylethenes for bioconjugation and fluorescence microscopy of cellular structures.
Authors: Uno, Kakishi and Aktalay, Ayse and Bossi, Mariano L and Irie, Masahiro and Belov, Vladimir N and Hell, Stefan W
Journal: Proceedings of the National Academy of Sciences of the United States of America (2021)
Authors: Uno, Kakishi and Aktalay, Ayse and Bossi, Mariano L and Irie, Masahiro and Belov, Vladimir N and Hell, Stefan W
Journal: Proceedings of the National Academy of Sciences of the United States of America (2021)
Comparison of cRGDfK Peptide Probes with Appended Shielded Heptamethine Cyanine Dye (s775z) for Near Infrared Fluorescence Imaging of Cancer.
Authors: Gamage, Rananjaya S and Li, Dong-Hao and Schreiber, Cynthia L and Smith, Bradley D
Journal: ACS omega (2021): 30130-30139
Authors: Gamage, Rananjaya S and Li, Dong-Hao and Schreiber, Cynthia L and Smith, Bradley D
Journal: ACS omega (2021): 30130-30139
Controlling fluorescence resonance energy transfer of donor-acceptor dyes by Diels-Alder dynamic covalent bonds.
Authors: Cui, Yanhui and Li, Fen and Zhang, Xin
Journal: Chemical communications (Cambridge, England) (2021): 3275-3278
Authors: Cui, Yanhui and Li, Fen and Zhang, Xin
Journal: Chemical communications (Cambridge, England) (2021): 3275-3278
Fluorescence correlation spectroscopy as a tool to investigate the directionality of proteolysis.
Authors: Sengupta, Bhaswati and Das, Nilimesh and Singh, Virender and Thakur, Ashwani K and Sen, Pratik
Journal: International journal of biological macromolecules (2020): 2524-2534
Authors: Sengupta, Bhaswati and Das, Nilimesh and Singh, Virender and Thakur, Ashwani K and Sen, Pratik
Journal: International journal of biological macromolecules (2020): 2524-2534
Application notes
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
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