Name: iFluor® 647 maleimide
Brand: AAT Bioquest
SKU: 1065
Price: 237 USD
Availability: InStock

iFluor® 647 maleimide

iFluor® 647 maleimide is a far-red fluorescent, thiol-reactive dye designed for covalent labeling of proteins, antibodies, and other sulfhydryl-containing biomolecules.

Thiol-Reactive Labeling: Selectively reacts with sulfhydryl groups, including cysteine residues.
Spectral Properties: Excitation/emission maxima of 655/670 nm.
pH Stability: Exhibits pH-independent fluorescence from pH 3 to 11.
Dye Compatibility: Spectrally similar to Cy5® and Alexa Fluor® 647, with greater brightness and photostability than Cy5®.

<p>HeLa cells were stained with mouse anti-tubulin followed with iFluor<sup>TM</sup> 647 goat anti-mouse IgG (H+L) (red); and nuclei were stained with DAPI (blue).</p>

Protocol

SDS

Catalog	Size	Price	Quantity
1065	1 mg	Price

Overview

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 647 dyes have fluorescence excitation and emission maxima of ~656 nm and ~670 nm respectively. The iFluor® 647 family has spectral properties essentially identical to those of Cy5® (Cy5® is the trademark of GE Healthcare). Compared to Cy5® probes, iFluor® 647 reagents have much stronger fluorescence and higher photostability. Their fluorescence is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family an excellent alternative to Cy5® and Alexa Fluor® 647 (Cy5® and Alexa Fluor® are the trademarks of GE Health Care and Invitrogen). iFluor® 647 maleimide is reasonably stable and shows good reactivity and selectivity with the thiol group.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 647 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	82.553 µL	412.763 µL	825.525 µL	4.128 mL	8.255 mL
5 mM	16.511 µL	82.553 µL	165.105 µL	825.525 µL	1.651 mL
10 mM	8.255 µL	41.276 µL	82.553 µL	412.763 µL	825.525 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1211.35 g/mol	=	mL	x	mM	=	mmol

Citations

View all 42 citations: Citation Explorer

Injection-free, spatially controlled delivery of glioblastoma cells to human brain slice cultures using soft cryogel biomaterials

Authors:

Hill, Sophie and Mohammed, Aso Omer and Ganser, Laurin and Sarker, Sabarni and Wang, Yu and Mammadsalahova, Pakiza and Al Shhab, Mohammad and Fertala, Nicole and Welzel, Petra B and Werner, Carsten and others,

Journal:

Biomaterials Advances (2026): 215226

Structural and mechanistic insights into the fungal glycosylphosphatidylinositol mannosyltransferase I complex

Authors:

Liu, Jia and Yang, Yizheng and Tan, Yi and Hua, Zhengkang and Hu, Xinlin and Ding, Xuyang and Yang, Ping and Ke, Yan and Zhang, Zhentao and Li, Tianlu and others,

Journal:

Proceedings of the National Academy of Sciences (2026): e2617140123

Architecture and mechanism of the human p24 cargo receptor

Authors:

Hua, Zhengkang and Zhang, Di and Zhang, Min and Hu, Xinlin and Kang, Chengtao and Sun, Huanhuan and Yang, Ping and Qi, Chu and Lin, Xinyuan and Li, Jiameng and others,

Journal:

Proceedings of the National Academy of Sciences (2026): e2610235123

Atomic Force Microscopy Based Single Molecule Recognition: Deciphering Biological Interactions at the Nanoscale

Authors:

Zhang, Huaiwei and Xu, Qianhui and Chen, Junmei and Chen, Danhong and Zhong, Haijian and Zhao, Weidong

Journal:

Journal of Molecular Recognition (2026): e70042

Immune modulatory oncolytic virus for osteosarcoma therapy

Authors:

Khan, Sumbul and Higgins, Theresa A and Shimko-Lofano, Isabella and Sandey, Maninder and Logsdon, Emma and Bunch, Gracie and Fatemi, Yasin and Hoffman, Leah MK and Smith, Bruce F and Agarwal, Payal

References

View all 49 references: Citation Explorer

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Authors:

Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.

Journal:

Zhen Ci Yan Jiu (2011): 262

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes

Authors:

Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.

Journal:

Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling

Authors:

Zhang S, Tan HC, Ooi EE.

Journal:

J Vis Exp. (2011)

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells

Authors:

Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.

Journal:

Biochem Biophys Res Commun (2011): 211

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers

Authors:

Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.

Journal:

J Microbiol Methods (2011): 119

Physical properties

Molecular weight	1211.35
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.03
Correction factor (280 nm)	0.03
Correction factor (656 nm)	0.0793
Extinction coefficient (cm ^-1 M ^-1)	220000 ¹
Excitation (nm)	655
Emission (nm)	670
Quantum yield	0.25 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 28, 2026