iFluor® 546 maleimide

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. iFluor® 546 is optimized to be well excited by the 546 nm laser or LED light sources, enabling new biological applications for fluorescence instruments equipped with the 546 nm laser or LED light sources. iFluor® 546 dye might be the brightest fluorophore available that is well excited by the 546 nm laser or LED light sources. iFluor® 546 maleimide is stable and shows good reactivity and selectivity with the thiol group.

Fluorescent dye maleimides are the most popular tool for conjugating dyes to a peptide, protein, antibody, thiol-modified oligonucleotide or nucleic acid through their SH group. Maleimides react readily with the thiol group of proteins, thiol-modified oligonucleotides, and other thiol-containing molecules under neutral conditions. The resulting dye conjugates are quite stable.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
1059	1 mg	Price

References

View all 47 references: Citation Explorer

Group V Secretory Phospholipase A2 Regulates Endocytosis of Acetylated LDL by Transcriptional Activation of PGK1 in RAW264.7 Macrophage Cell Line.

Authors:

Fujioka, Daisuke and Watanabe, Yosuke and Nakamura, Takamitsu and Yokoyama, Takashi and Miyazawa, Keiji and Murakami, Makoto and Kugiyama, Kiyotaka

Journal:

Journal of atherosclerosis and thrombosis (2021)

Quantitative Assessment of Tendon Hierarchical Structure by Combined Second Harmonic Generation and Immunofluorescence Microscopy.

Authors:

Durgam, Sushmitha and Singh, Benjamin and Cole, Sara L and Brokken, Matthew T and Stewart, Matthew

Journal:

Tissue engineering. Part C, Methods (2020): 253-262

Surface passivation of zero-mode waveguide nanostructures: benchmarking protocols and fluorescent labels.

Authors:

Patra, Satyajit and Baibakov, Mikhail and Claude, Jean-Benoît and Wenger, Jérôme

Journal:

Scientific reports (2020): 5235

Long-Range Single-Molecule Förster Resonance Energy Transfer between Alexa Dyes in Zero-Mode Waveguides.

Authors:

Baibakov, Mikhail and Patra, Satyajit and Claude, Jean-Benoît and Wenger, Jérôme

Journal:

ACS omega (2020): 6947-6955

Curare alkaloids from Matis Dart Poison: Comparison with d-tubocurarine in interactions with nicotinic, 5-HT3 serotonin and GABAA receptors.

Authors:

Spirova, Ekaterina N and Ivanov, Igor A and Kasheverov, Igor E and Kudryavtsev, Denis S and Shelukhina, Irina V and Garifulina, Alexandra I and Son, Lina V and Lummis, Sarah C R and Malca-Garcia, Gonzalo R and Bussmann, Rainer W and Hennig, Lothar and Giannis, Athanassios and Tsetlin, Victor I

Journal:

PloS one (2019): e0210182

Physical properties

Molecular weight	1082.10
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.25
Correction factor (280 nm)	0.15
Extinction coefficient (cm ^-1 M ^-1)	100000 ¹
Excitation (nm)	541
Emission (nm)	557
Quantum yield	0.67 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 13, 2025