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iFluor® 546 maleimide
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1082.10 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (260 nm) | 0.25 |
| Correction Factor (280 nm) | 0.15 |
| Extinction coefficient (cm -1 M -1) | 1000001 |
| Excitation (nm) | 541 |
| Emission (nm) | 557 |
| Quantum yield | 0.671 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Alternative formats
| iFluor® 546 succinimidyl ester |
| Overview |  SDSProtocol |
See also: iFluor® Dyes and Kits
Molecular weight 1082.10 | Correction Factor (260 nm) 0.25 | Correction Factor (280 nm) 0.15 | Extinction coefficient (cm -1 M -1) 1000001 | Excitation (nm) 541 | Emission (nm) 557 | Quantum yield 0.671 |
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. iFluor® 546 is optimized to be well excited by the 546 nm laser or LED light sources, enabling new biological applications for fluorescence instruments equipped with the 546 nm laser or LED light sources. iFluor® 546 dye might be the brightest fluorophore available that is well excited by the 546 nm laser or LED light sources. iFluor® 546 maleimide is stable and shows good reactivity and selectivity with the thiol group.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 546 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 92.413 µL | 462.065 µL | 924.129 µL | 4.621 mL | 9.241 mL |
| 5 mM | 18.483 µL | 92.413 µL | 184.826 µL | 924.129 µL | 1.848 mL |
| 10 mM | 9.241 µL | 46.206 µL | 92.413 µL | 462.065 µL | 924.129 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.25 |
| Correction Factor (280 nm) | 0.15 |
| Extinction coefficient (cm -1 M -1) | 1000001 |
| Excitation (nm) | 541 |
| Emission (nm) | 557 |
| Quantum yield | 0.671 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| iFluor® 350 maleimide | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
| iFluor® 488 maleimide | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
| iFluor® 555 maleimide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| iFluor® 647 maleimide | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
| iFluor® 680 maleimide | 684 | 701 | 2200001 | 0.231 | 0.097 | 0.094 |
| iFluor® 700 maleimide | 690 | 713 | 2200001 | 0.231 | 0.09 | 0.04 |
| iFluor® 750 maleimide | 757 | 779 | 2750001 | 0.121 | 0.044 | 0.039 |
| iFluor® 790 maleimide | 787 | 812 | 2500001 | 0.131 | 0.1 | 0.09 |
| iFluor® 800 maleimide | 801 | 820 | 2500001 | 0.111 | 0.03 | 0.08 |
Show More (22) | ||||||
Images
Figure 1. Chemical structure for iFluor® 546 maleimide.
References
View all 47 references: Citation Explorer
Group V Secretory Phospholipase A2 Regulates Endocytosis of Acetylated LDL by Transcriptional Activation of PGK1 in RAW264.7 Macrophage Cell Line.
Authors: Fujioka, Daisuke and Watanabe, Yosuke and Nakamura, Takamitsu and Yokoyama, Takashi and Miyazawa, Keiji and Murakami, Makoto and Kugiyama, Kiyotaka
Journal: Journal of atherosclerosis and thrombosis (2021)
Authors: Fujioka, Daisuke and Watanabe, Yosuke and Nakamura, Takamitsu and Yokoyama, Takashi and Miyazawa, Keiji and Murakami, Makoto and Kugiyama, Kiyotaka
Journal: Journal of atherosclerosis and thrombosis (2021)
Quantitative Assessment of Tendon Hierarchical Structure by Combined Second Harmonic Generation and Immunofluorescence Microscopy.
Authors: Durgam, Sushmitha and Singh, Benjamin and Cole, Sara L and Brokken, Matthew T and Stewart, Matthew
Journal: Tissue engineering. Part C, Methods (2020): 253-262
Authors: Durgam, Sushmitha and Singh, Benjamin and Cole, Sara L and Brokken, Matthew T and Stewart, Matthew
Journal: Tissue engineering. Part C, Methods (2020): 253-262
Surface passivation of zero-mode waveguide nanostructures: benchmarking protocols and fluorescent labels.
Authors: Patra, Satyajit and Baibakov, Mikhail and Claude, Jean-Benoît and Wenger, Jérôme
Journal: Scientific reports (2020): 5235
Authors: Patra, Satyajit and Baibakov, Mikhail and Claude, Jean-Benoît and Wenger, Jérôme
Journal: Scientific reports (2020): 5235
Long-Range Single-Molecule Förster Resonance Energy Transfer between Alexa Dyes in Zero-Mode Waveguides.
Authors: Baibakov, Mikhail and Patra, Satyajit and Claude, Jean-Benoît and Wenger, Jérôme
Journal: ACS omega (2020): 6947-6955
Authors: Baibakov, Mikhail and Patra, Satyajit and Claude, Jean-Benoît and Wenger, Jérôme
Journal: ACS omega (2020): 6947-6955
Curare alkaloids from Matis Dart Poison: Comparison with d-tubocurarine in interactions with nicotinic, 5-HT3 serotonin and GABAA receptors.
Authors: Spirova, Ekaterina N and Ivanov, Igor A and Kasheverov, Igor E and Kudryavtsev, Denis S and Shelukhina, Irina V and Garifulina, Alexandra I and Son, Lina V and Lummis, Sarah C R and Malca-Garcia, Gonzalo R and Bussmann, Rainer W and Hennig, Lothar and Giannis, Athanassios and Tsetlin, Victor I
Journal: PloS one (2019): e0210182
Authors: Spirova, Ekaterina N and Ivanov, Igor A and Kasheverov, Igor E and Kudryavtsev, Denis S and Shelukhina, Irina V and Garifulina, Alexandra I and Son, Lina V and Lummis, Sarah C R and Malca-Garcia, Gonzalo R and Bussmann, Rainer W and Hennig, Lothar and Giannis, Athanassios and Tsetlin, Victor I
Journal: PloS one (2019): e0210182
Time-Gated FRET and DNA-Based Photonic Molecular Logic Gates: AND, OR, NAND, and NOR.
Authors: Massey, Melissa and Medintz, Igor L and Ancona, Mario G and Algar, W Russ
Journal: ACS sensors (2017): 1205-1214
Authors: Massey, Melissa and Medintz, Igor L and Ancona, Mario G and Algar, W Russ
Journal: ACS sensors (2017): 1205-1214
Photoinduced apoptosis using a peptide carrying a photosensitizer.
Authors: Watanabe, Kazunori and Fujiwara, Hayato and Kitamatsu, Mizuki and Ohtsuki, Takashi
Journal: Bioorganic & medicinal chemistry letters (2016): 3115-3118
Authors: Watanabe, Kazunori and Fujiwara, Hayato and Kitamatsu, Mizuki and Ohtsuki, Takashi
Journal: Bioorganic & medicinal chemistry letters (2016): 3115-3118
Alexa fluor-labeled fluorescent cellulose nanocrystals for bioimaging solid cellulose in spatially structured microenvironments.
Authors: Grate, Jay W and Mo, Kai-For and Shin, Yongsoon and Vasdekis, Andreas and Warner, Marvin G and Kelly, Ryan T and Orr, Galya and Hu, Dehong and Dehoff, Karl J and Brockman, Fred J and Wilkins, Michael J
Journal: Bioconjugate chemistry (2015): 593-601
Authors: Grate, Jay W and Mo, Kai-For and Shin, Yongsoon and Vasdekis, Andreas and Warner, Marvin G and Kelly, Ryan T and Orr, Galya and Hu, Dehong and Dehoff, Karl J and Brockman, Fred J and Wilkins, Michael J
Journal: Bioconjugate chemistry (2015): 593-601
The interaction affinity between vascular cell adhesion molecule-1 (VCAM-1) and very late antigen-4 (VLA-4) analyzed by quantitative FRET.
Authors: Chakraborty, Sandeep and Hu, Shih-Yang and Wu, Shu-Han and Karmenyan, Artashes and Chiou, Arthur
Journal: PloS one (2015): e0121399
Authors: Chakraborty, Sandeep and Hu, Shih-Yang and Wu, Shu-Han and Karmenyan, Artashes and Chiou, Arthur
Journal: PloS one (2015): e0121399
Photosensitizer and polycationic peptide-labeled streptavidin as a nano-carrier for light-controlled protein transduction.
Authors: Minamihata, Kosuke and Maeda, Yasukazu and Yamaguchi, Satoshi and Ishihara, Wataru and Ishiwatari, Akira and Takamori, Satoshi and Yamahira, Shinya and Nagamune, Teruyuki
Journal: Journal of bioscience and bioengineering (2015): 630-6
Authors: Minamihata, Kosuke and Maeda, Yasukazu and Yamaguchi, Satoshi and Ishihara, Wataru and Ishiwatari, Akira and Takamori, Satoshi and Yamahira, Shinya and Nagamune, Teruyuki
Journal: Journal of bioscience and bioengineering (2015): 630-6
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FAQ
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How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?