iFluor® 546 maleimide

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	1082.10
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.25
Correction Factor (280 nm)	0.15
Extinction coefficient (cm ^-1 M ^-1)	100000¹
Excitation (nm)	541
Emission (nm)	557
Quantum yield	0.67¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Alternative formats

iFluor® 546 succinimidyl ester

Overview SDS Protocol

Molecular weight

1082.10

Correction Factor (260 nm)

0.25

Correction Factor (280 nm)

0.15

Extinction coefficient (cm ^-1 M ^-1)

100000¹

Excitation (nm)

541

Emission (nm)

557

Quantum yield

0.67¹

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. iFluor® 546 is optimized to be well excited by the 546 nm laser or LED light sources, enabling new biological applications for fluorescence instruments equipped with the 546 nm laser or LED light sources. iFluor® 546 dye might be the brightest fluorophore available that is well excited by the 546 nm laser or LED light sources. iFluor® 546 maleimide is stable and shows good reactivity and selectivity with the thiol group.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 546 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	92.413 µL	462.065 µL	924.129 µL	4.621 mL	9.241 mL
5 mM	18.483 µL	92.413 µL	184.826 µL	924.129 µL	1.848 mL
10 mM	9.241 µL	46.206 µL	92.413 µL	462.065 µL	924.129 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.25
Correction Factor (280 nm)	0.15
Extinction coefficient (cm ^-1 M ^-1)	100000¹
Excitation (nm)	541
Emission (nm)	557
Quantum yield	0.67¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 350 maleimide	345	450	20000¹	0.95¹	0.83	0.23
iFluor® 488 maleimide	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 555 maleimide	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 647 maleimide	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 680 maleimide	684	701	220000¹	0.23¹	0.097	0.094
iFluor® 700 maleimide	690	713	220000¹	0.23¹	0.09	0.04
iFluor® 750 maleimide	757	779	275000¹	0.12¹	0.044	0.039
iFluor® 790 maleimide	787	812	250000¹	0.13¹	0.1	0.09
iFluor® 800 maleimide	801	820	250000¹	0.11¹	0.03	0.08
iFluor® 810 maleimide	811	822	250000¹	0.05¹	0.09	0.15
iFluor® 820 maleimide	822	850	250000¹		0.11	0.16
iFluor® 860 maleimide	853	878	250000¹		0.1	0.14
iFluor® 532 maleimide	537	560	90000¹	0.68¹	0.26	0.16
iFluor® 594 maleimide	588	604	180000¹	0.53¹	0.05	0.04
iFluor® 405 maleimide	403	427	37000¹	0.91¹	0.48	0.77
iFluor® 430 maleimide	433	498	40000¹	0.78¹	0.68	0.3
iFluor® 568 maleimide	568	587	100000¹	0.57¹	0.34	0.15
iFluor® 633 maleimide	640	654	250000¹	0.29¹	0.062	0.044
iFluor® 450 maleimide	451	502	40000¹	0.82¹	0.45	0.27
iFluor® 546 Styramide Superior Replacement for Alexa Fluor 546 tyramide	541	557	100000¹	0.67¹	0.25	0.15
iFluor® 546 Tyramide	541	557	100000¹	0.67¹	0.25	0.15
iFluor® 460 maleimide	468	493	80000¹	~0.8¹	0.98	0.46
iFluor® 665 maleimide	667	692	110,000¹	0.22¹	0.12	0.09
iFluor® 840 maleimide	836	879	200000¹	-	0.2	0.09
iFluor® 770 maleimide	777	797	250000¹	0.16	0.09	0.08
iFluor® 780 maleimide	784	808	250000¹	0.16¹	0.13	0.12
iFluor® 830 maleimide	830	867	-	-	-	-
iFluor® 514 maleimide	511	527	75000¹	0.83¹	0.265	0.116
iFluor® 660 maleimide	663	678	250000¹	0.26¹	0.07	0.08
iFluor® 670 maleimide	671	682	200000¹	0.55¹	0.03	0.033
Show More (21)

Images

Figure 1. Chemical structure for iFluor® 546 maleimide.

References

View all 47 references: Citation Explorer

Group V Secretory Phospholipase A2 Regulates Endocytosis of Acetylated LDL by Transcriptional Activation of PGK1 in RAW264.7 Macrophage Cell Line.
Authors: Fujioka, Daisuke and Watanabe, Yosuke and Nakamura, Takamitsu and Yokoyama, Takashi and Miyazawa, Keiji and Murakami, Makoto and Kugiyama, Kiyotaka
Journal: Journal of atherosclerosis and thrombosis (2021)

Quantitative Assessment of Tendon Hierarchical Structure by Combined Second Harmonic Generation and Immunofluorescence Microscopy.
Authors: Durgam, Sushmitha and Singh, Benjamin and Cole, Sara L and Brokken, Matthew T and Stewart, Matthew
Journal: Tissue engineering. Part C, Methods (2020): 253-262

Surface passivation of zero-mode waveguide nanostructures: benchmarking protocols and fluorescent labels.
Authors: Patra, Satyajit and Baibakov, Mikhail and Claude, Jean-Benoît and Wenger, Jérôme
Journal: Scientific reports (2020): 5235

Long-Range Single-Molecule Förster Resonance Energy Transfer between Alexa Dyes in Zero-Mode Waveguides.
Authors: Baibakov, Mikhail and Patra, Satyajit and Claude, Jean-Benoît and Wenger, Jérôme
Journal: ACS omega (2020): 6947-6955

Curare alkaloids from Matis Dart Poison: Comparison with d-tubocurarine in interactions with nicotinic, 5-HT3 serotonin and GABAA receptors.
Authors: Spirova, Ekaterina N and Ivanov, Igor A and Kasheverov, Igor E and Kudryavtsev, Denis S and Shelukhina, Irina V and Garifulina, Alexandra I and Son, Lina V and Lummis, Sarah C R and Malca-Garcia, Gonzalo R and Bussmann, Rainer W and Hennig, Lothar and Giannis, Athanassios and Tsetlin, Victor I
Journal: PloS one (2019): e0210182

Time-Gated FRET and DNA-Based Photonic Molecular Logic Gates: AND, OR, NAND, and NOR.
Authors: Massey, Melissa and Medintz, Igor L and Ancona, Mario G and Algar, W Russ
Journal: ACS sensors (2017): 1205-1214

Photoinduced apoptosis using a peptide carrying a photosensitizer.
Authors: Watanabe, Kazunori and Fujiwara, Hayato and Kitamatsu, Mizuki and Ohtsuki, Takashi
Journal: Bioorganic & medicinal chemistry letters (2016): 3115-3118

Alexa fluor-labeled fluorescent cellulose nanocrystals for bioimaging solid cellulose in spatially structured microenvironments.
Authors: Grate, Jay W and Mo, Kai-For and Shin, Yongsoon and Vasdekis, Andreas and Warner, Marvin G and Kelly, Ryan T and Orr, Galya and Hu, Dehong and Dehoff, Karl J and Brockman, Fred J and Wilkins, Michael J
Journal: Bioconjugate chemistry (2015): 593-601

The interaction affinity between vascular cell adhesion molecule-1 (VCAM-1) and very late antigen-4 (VLA-4) analyzed by quantitative FRET.
Authors: Chakraborty, Sandeep and Hu, Shih-Yang and Wu, Shu-Han and Karmenyan, Artashes and Chiou, Arthur
Journal: PloS one (2015): e0121399

Photosensitizer and polycationic peptide-labeled streptavidin as a nano-carrier for light-controlled protein transduction.
Authors: Minamihata, Kosuke and Maeda, Yasukazu and Yamaguchi, Satoshi and Ishihara, Wataru and Ishiwatari, Akira and Takamori, Satoshi and Yamahira, Shinya and Nagamune, Teruyuki
Journal: Journal of bioscience and bioengineering (2015): 630-6