iFluor® 546 maleimide

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. iFluor® 546 is optimized to be well excited by the 546 nm laser or LED light sources, enabling new biological applications for fluorescence instruments equipped with the 546 nm laser or LED light sources. iFluor® 546 dye might be the brightest fluorophore available that is well excited by the 546 nm laser or LED light sources. iFluor® 546 maleimide is stable and shows good reactivity and selectivity with the thiol group.

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 350 maleimide	345	450	20000¹	0.95¹	0.83	0.23
iFluor® 405 maleimide	403	427	37000¹	0.91¹	0.48	0.77
iFluor® 430 maleimide	433	498	40000¹	0.78¹	0.68	0.3
iFluor® 450 maleimide	451	502	40000¹	0.82¹	0.45	0.27
iFluor® 460 maleimide	468	493	80000¹	~0.8¹	0.98	0.46
iFluor® 488 maleimide	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 510 maleimide	511	530	-	-	-	-
iFluor® 514 maleimide	511	527	75000¹	0.83¹	0.265	0.116
iFluor® 532 maleimide	537	560	90000¹	0.68¹	0.26	0.16
iFluor® 540 maleimide	540	557	-	-	-	0.105
iFluor® 546 Styramide Superior Replacement for Alexa Fluor 546 tyramide	541	557	100000¹	0.67¹	0.25	0.15
iFluor® 555 maleimide	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 560 maleimide	560	571	120000¹	0.57¹	0.0482	0.069
iFluor® 568 maleimide	568	587	100000¹	0.57¹	0.34	0.15
iFluor® 594 maleimide	587	603	200000¹	0.53¹	0.05	0.04
iFluor® 605 maleimide	603	623	-	-	-	-
iFluor® 625 maleimide	624	640	-	-	-	-
iFluor® 633 maleimide	640	654	250000¹	0.29¹	0.062	0.044
iFluor® 647 maleimide	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 660 maleimide	663	678	250000¹	0.26¹	0.07	0.08
iFluor® 665 maleimide	667	692	110,000¹	0.22¹	0.12	0.09
iFluor® 670 maleimide	671	682	200000¹	0.55¹	0.03	0.033
iFluor® 680 maleimide	684	701	220000¹	0.23¹	0.097	0.094
iFluor® 700 maleimide	690	713	220000¹	0.23¹	0.09	0.04
iFluor® 720 maleimide	716	740	240000¹	0.14¹	0.15	0.13
iFluor® 750 maleimide	757	779	275000¹	0.12¹	0.044	0.039
iFluor® 770 maleimide	777	797	250000¹	0.16	0.09	0.08
iFluor® 780 maleimide	784	808	250000¹	0.16¹	0.13	0.12
iFluor® 790 maleimide	787	812	250000¹	0.13¹	0.1	0.09
iFluor® 800 maleimide	801	820	250000¹	0.11¹	0.03	0.08
iFluor® 810 maleimide	811	822	250000¹	0.05¹	0.09	0.15
iFluor® 820 maleimide	822	850	250000¹		0.11	0.16
iFluor® 830 maleimide	830	867	-	-	-	-
iFluor® 840 maleimide	836	879	200000¹	-	0.2	0.09
iFluor® 860 maleimide	853	878	250000¹		0.1	0.14
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References

View all 47 references: Citation Explorer

Group V Secretory Phospholipase A2 Regulates Endocytosis of Acetylated LDL by Transcriptional Activation of PGK1 in RAW264.7 Macrophage Cell Line.
Authors: Fujioka, Daisuke and Watanabe, Yosuke and Nakamura, Takamitsu and Yokoyama, Takashi and Miyazawa, Keiji and Murakami, Makoto and Kugiyama, Kiyotaka
Journal: Journal of atherosclerosis and thrombosis (2021)

Quantitative Assessment of Tendon Hierarchical Structure by Combined Second Harmonic Generation and Immunofluorescence Microscopy.
Authors: Durgam, Sushmitha and Singh, Benjamin and Cole, Sara L and Brokken, Matthew T and Stewart, Matthew
Journal: Tissue engineering. Part C, Methods (2020): 253-262

Surface passivation of zero-mode waveguide nanostructures: benchmarking protocols and fluorescent labels.
Authors: Patra, Satyajit and Baibakov, Mikhail and Claude, Jean-Benoît and Wenger, Jérôme
Journal: Scientific reports (2020): 5235

Long-Range Single-Molecule Förster Resonance Energy Transfer between Alexa Dyes in Zero-Mode Waveguides.
Authors: Baibakov, Mikhail and Patra, Satyajit and Claude, Jean-Benoît and Wenger, Jérôme
Journal: ACS omega (2020): 6947-6955

Curare alkaloids from Matis Dart Poison: Comparison with d-tubocurarine in interactions with nicotinic, 5-HT3 serotonin and GABAA receptors.
Authors: Spirova, Ekaterina N and Ivanov, Igor A and Kasheverov, Igor E and Kudryavtsev, Denis S and Shelukhina, Irina V and Garifulina, Alexandra I and Son, Lina V and Lummis, Sarah C R and Malca-Garcia, Gonzalo R and Bussmann, Rainer W and Hennig, Lothar and Giannis, Athanassios and Tsetlin, Victor I
Journal: PloS one (2019): e0210182

Page updated on May 19, 2025

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Catalog Number	1059
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Physical properties

Molecular weight	1082.10
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.25
Correction Factor (280 nm)	0.15
Extinction coefficient (cm ^-1 M ^-1)	100000¹
Excitation (nm)	541
Emission (nm)	557
Quantum yield	0.67¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501