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iFluor® 700 maleimide
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 700 dyes have fluorescence excitation and emission maxima of ~690 nm and ~713 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 700 labeling dyes (Alexa Fluor® is the trademark of Invitrogen). iFluor® 700 maleimide is reasonably stable and shows good reactivity and selectivity with the thiol group.
<p>Flow cytometric analysis of APC-iFluor®700&nbsp; (Red Bar) or APC-Alexa Fluor&reg; 700&nbsp; (Blue Bar)&nbsp; anti-human CD8 on human lymphocytes. Whole blood was stained with APC-iFluor®700&nbsp; or&nbsp; APC-Alexa Fluor&reg; 700&nbsp; anti-human CD8&nbsp; and compared to whole blood stained with a APC-iFluor®700 and APC-Alexa Fluor&reg; 700 mouse IgG control. Flow cytometry was performed on a ACEA flow cytometry system.</p>
<p>Flow cytometric analysis of APC-iFluor®700&nbsp; (Red Bar) or APC-Alexa Fluor&reg; 700&nbsp; (Blue Bar)&nbsp; anti-human CD8 on human lymphocytes. Whole blood was stained with APC-iFluor®700&nbsp; or&nbsp; APC-Alexa Fluor&reg; 700&nbsp; anti-human CD8&nbsp; and compared to whole blood stained with a APC-iFluor®700 and APC-Alexa Fluor&reg; 700 mouse IgG control. Flow cytometry was performed on a ACEA flow cytometry system.</p>
CatalogSize
Price
Quantity
10671 mg
Price
 
Physical properties

Molecular weight1086.12
SolventDMSO
Spectral properties

Correction factor (260 nm)0.09
Correction factor (280 nm)0.04
Correction factor (565 nm)0.0766
Correction factor (650 nm)0.4004
Extinction coefficient (cm -1 M -1)
220000
1
Excitation (nm)690
Emission (nm)713
Quantum yield
0.23
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 8, 2025