iFluor® 350 acid

• High performance: Brighter fluorescence than Alexa Fluor® 350 and AMCA, with excellent photostability.
• Direct equivalent: Serves as a high-performance replacement for Alexa Fluor® 350 acid, with enhanced solubility and pH stability.
• Conjugation-ready chemistry: Can be converted into reactive esters for coupling to proteins, peptides, and oligonucleotides.
• Versatile applications: Suitable for ICC, flow cytometry, HCS, live-cell imaging, and fluorescence microscopy.

iFluor® 350 is a blue-fluorescent dye with spectral properties comparable to AMCA and Alexa Fluor® 350, but with superior photostability, brightness, and water solubility. With excitation and emission maxima at 345/450 nm, it is highly compatible with UV laser excitation sources and well-suited for applications requiring strong, stable fluorescence in the blue channel. The dye performs reliably across a broad pH range (pH 3 to 11), making it effective under diverse experimental conditions.

iFluor® 350 acid is the non-reactive form of the dye and is ideal for use as a fluorescence reference standard or control in assays involving iFluor® 350 conjugates. It is suitable for a wide range of imaging and detection techniques, including confocal microscopy, immunocytochemistry, live-cell imaging, and flow cytometry. It can also be chemically modified to generate activated esters or STP derivatives and coupled to hydrazines, hydroxylamines, or amines using water-soluble carbodiimides such as EDAC, enabling efficient labeling of proteins, antibodies, peptides, and amine-modified oligonucleotides.