iFluor® 770 maleimide

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 355, 405, 488, 555, 633, 638, 647, 660, and 802 nm). iFluor® 770 dyes have fluorescence excitation and emission maxima of ~777 nm and ~797 nm respectively. These spectral characteristics make them a unique color for fluorescence imaging and flow cytometry applications. iFluor® 770 is an excellent acceptor dye for preparing tandem colors with APC and PE. These iFluor® 770 tandem colors offer a set of unique color profiles for spectral flow cytometry. iFluor® 770 is one of the brightest NIR dyes, and some of its antibody conjugates are significantly brighter than those prepared with IRDyes of similar wavelengths, such as IRDye 800RS. iFluor® 770 maleimide is a thiol-reactive form used to conjugate with thiol-containing molecules such as reduced antibodies, thiol-modified oligos, and peptides.

Fluorescent dye maleimides are the most popular tool for conjugating dyes to a peptide, protein, antibody, thiol-modified oligonucleotide, or nucleic acid through their SH group. Maleimides react readily with the thiol group of proteins, thiol-modified oligonucleotides, and other thiol-containing molecules under neutral conditions. The resulting dye conjugates are quite stable.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
1370	1 mg	Price

References

View all 5 references: Citation Explorer

Two-photon peak molecular brightness spectra reveal long-wavelength enhancements of multiplexed imaging depth and photostability.

Authors:

Lang, Ryan T and Spring, Bryan Q

Journal:

Biomedical optics express (2021): 5909-5919

Preliminary study on the application of en bloc resection combined with near-infrared molecular imaging technique in the diagnosis and treatment of bladder cancer.

Authors:

Yang, Yongjun and Yang, Xiaofeng and Liu, Chao and Li, Jiawei

Journal:

World journal of urology (2020)

Enhanced broadband fluorescence detection of nucleic acids using multipolar gap-plasmons on biomimetic Au metasurfaces.

Authors:

Narasimhan, Vinayak and Siddique, Radwanul Hasan and Hoffmann, Magnus and Kumar, Shailabh and Choo, Hyuck

Journal:

Nanoscale (2019): 13750-13757

Effect of Liposomes with Different Double Arms Polyethyleneglycol on Hepatic Metastasis Model Mice and Evaluation Using a Fluorescent Imaging Device.

Authors:

Sugiyama, Ikumi and Oikawa, Hiroki and Masuda, Tomoyuki and Sadzuka, Yasuyuki

Journal:

Current drug delivery (2017): 668-675

Au nanostructures by colloidal lithography: from quenching to extensive fluorescence enhancement.

Authors:

Xie, Fang and Centeno, Anthony and Ryan, Mary R and Riley, D Jason and Alford, Neil M

Journal:

Journal of materials chemistry. B (2013): 536-543

Physical properties

Solvent

DMSO

Spectral properties

Absorbance (nm)	772
Correction factor (260 nm)	0.09
Correction factor (280 nm)	0.08
Extinction coefficient (cm ^-1 M ^-1)	250000 ¹
Excitation (nm)	777
Emission (nm)	797
Quantum yield	0.16

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on September 29, 2025