iFluor® 660 maleimide

iFluor® 660 dyes have fluorescence excitation and emission maxima of ~660 nm and ~680 nm respectively. These spectral characteristics make them a unique acceptor for preparing PE, APC and PerCP tandems that are widely used in flow cytometry applications. iFluor® 660 Maleimide is stable and shows good reactivity and selectivity with protein thiol group.

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Physical properties

Molecular weight	1117.16
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.07
Correction Factor (280 nm)	0.08
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	663
Emission (nm)	678
Quantum yield	0.26¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Alternative formats

iFluor® 660 succinimidyl ester

iFluor® 660 amine

Overview SDS Protocol

Molecular weight

1117.16

Correction Factor (260 nm)

0.07

Correction Factor (280 nm)

0.08

Extinction coefficient (cm ^-1 M ^-1)

250000¹

Excitation (nm)

663

Emission (nm)

678

Quantum yield

0.26¹

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 514, 555, 633, 647 and 808 nm). iFluor® 660 dyes have fluorescence excitation and emission maxima of ~660 nm and ~680 nm respectively. These spectral characteristics make them a unique acceptor for preparing PE, APC and PerCP tandems that are widely used in flow cytometry applications. iFluor® 660 Maleimide is stable and shows good reactivity and selectivity with protein thiol group.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 660 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	89.513 µL	447.563 µL	895.127 µL	4.476 mL	8.951 mL
5 mM	17.903 µL	89.513 µL	179.025 µL	895.127 µL	1.79 mL
10 mM	8.951 µL	44.756 µL	89.513 µL	447.563 µL	895.127 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.07
Correction Factor (280 nm)	0.08
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	663
Emission (nm)	678
Quantum yield	0.26¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 350 maleimide	345	450	20000¹	0.95¹	0.83	0.23
iFluor® 488 maleimide	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 555 maleimide	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 647 maleimide	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 680 maleimide	684	701	220000¹	0.23¹	0.097	0.094
iFluor® 700 maleimide	690	713	220000¹	0.23¹	0.09	0.04
iFluor® 750 maleimide	757	779	275000¹	0.12¹	0.044	0.039
iFluor® 790 maleimide	787	812	250000¹	0.13¹	0.1	0.09
iFluor® 800 maleimide	801	820	250000¹	0.11¹	0.03	0.08
iFluor® 810 maleimide	811	822	250000¹	0.05¹	0.09	0.15
iFluor® 820 maleimide	822	850	250000¹		0.11	0.16
iFluor® 860 maleimide	853	878	250000¹		0.1	0.14
iFluor® 532 maleimide	537	560	90000¹	0.68¹	0.26	0.16
iFluor® 594 maleimide	588	604	180000¹	0.53¹	0.05	0.04
iFluor® 405 maleimide	403	427	37000¹	0.91¹	0.48	0.77
iFluor® 430 maleimide	433	498	40000¹	0.78¹	0.68	0.3
iFluor® 568 maleimide	568	587	100000¹	0.57¹	0.34	0.15
iFluor® 633 maleimide	640	654	250000¹	0.29¹	0.062	0.044
iFluor® 450 maleimide	451	502	40000¹	0.82¹	0.45	0.27
iFluor® 460 maleimide	468	493	80000¹	~0.8¹	0.98	0.46
iFluor® 665 maleimide	667	692	110,000¹	0.22¹	0.12	0.09
iFluor® 660 Styramide	663	678	250000¹	0.26¹	0.07	0.08
iFluor® 546 maleimide	541	557	100000¹	0.67¹	0.25	0.15
iFluor® 840 maleimide	836	879	200000¹	-	0.2	0.09
iFluor® 770 maleimide	777	797	250000¹	0.16	0.09	0.08
iFluor® 780 maleimide	784	808	250000¹	0.16¹	0.13	0.12
iFluor® 830 maleimide	830	867	-	-	-	-
iFluor® 514 maleimide	511	527	75000¹	0.83¹	0.265	0.116
iFluor® 670 maleimide	671	682	200000¹	0.55¹	0.03	0.033
Show More (20)

Images

Figure 1. iFluor® 660 dyes have fluorescence excitation and emission maxima of ~660 nm and ~680 nm respectively. These spectral characteristics make them a unique acceptor for preparing PE, APC and PerCP tandems that are widely used in flow cytometry applications. iFluor® 660 Maleimide is stable and shows good reactivity and selectivity with protein thiol group.

References

View all 31 references: Citation Explorer

Abortive ligation intermediate blocks seamless repair of double-stranded breaks.
Authors: Li, Xuegang and Jin, Jiacheng and Xu, Wenxuan and Wang, Mingdao and Liu, Liangwei
Journal: International journal of biological macromolecules (2022): 1498-1503

On-site rapid and simultaneous detection of acetamiprid and fipronil using a dual-fluorescence lab-on-fiber biosensor.
Authors: Song, Dan and Liu, Jiayao and Xu, Wenjuan and Han, Xiangzhi and Wang, Hongliang and Zhuo, Yuxin and Li, Chunsheng and Long, Feng
Journal: Mikrochimica acta (2022): 234

Mechanism of Cyanine5 to Cyanine3 Photoconversion and Its Application for High-Density Single-Particle Tracking in a Living Cell.
Authors: Cho, Yoonjung and An, Hyeong Jeon and Kim, Taehoon and Lee, Chulbom and Lee, Nam Ki
Journal: Journal of the American Chemical Society (2021): 14125-14135

Molecular and Spectroscopic Characterization of Green and Red Cyanine Fluorophores from the Alexa Fluor and AF Series*.
Authors: Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben
Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2021)

Restricted intramolecular rotation of fluorescent molecular rotors at the periphery of aqueous microdroplets in oil.
Authors: Kang, Jooyoun and Lhee, SangMoon and Lee, Jae Kyoo and Zare, Richard N and Nam, Hong Gil
Journal: Scientific reports (2020): 16859

Development of sesbania mosaic virus nanoparticles for imaging.
Authors: Vishnu Vardhan, G P and Hema, M and Sushmitha, C and Savithri, H S and Natraj, Usha and Murthy, M R N
Journal: Archives of virology (2019): 497-507

PET imaging of EGFR expression using an 18F-labeled RNA aptamer.
Authors: Cheng, Siyuan and Jacobson, Orit and Zhu, Guizhi and Chen, Zhen and Liang, Steve H and Tian, Rui and Yang, Zhen and Niu, Gang and Zhu, Xiaohua and Chen, Xiaoyuan
Journal: European journal of nuclear medicine and molecular imaging (2019): 948-956

Deciphering Design Principles of Förster Resonance Energy Transfer-Based Protease Substrates: Thermolysin-Like Protease from Geobacillus stearothermophilus as a Test Case.
Authors: Ripp, Sophie and Turunen, Petri and Minot, Ethan D and Rowan, Alan E and Blank, Kerstin G
Journal: ACS omega (2018): 4148-4156

Targetability of hyaluronic acid nanogel to cancer cells: In vitro and in vivo studies.
Authors: Pedrosa, S S and Pereira, P and Correia, A and Gama, F M
Journal: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences (2017): 102-113

[Zinc-dependent metalloprotease 1 (Zmp1) inhibits the proliferation and phagosome-lysosome fusion of mouse macrophage RAW264.7].
Authors: Fang, Chencheng and Zhang, Jiming and Lu, Nan and Li, Peng and Fan, Yu and Kang, Yuexi and Yang, Chun and He, Yonglin
Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (2016): 1317-1320

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iFluor® 660 maleimide

OverviewSDSProtocol

Calculators

Common stock solution preparation

Molarity calculator

Spectrum

Spectral properties

Product Family

Images

References

Documents

Overview SDS Protocol