iFluor® 660 maleimide
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1117.16 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.07 |
Correction Factor (280 nm) | 0.08 |
Extinction coefficient (cm -1 M -1) | 2500001 |
Excitation (nm) | 663 |
Emission (nm) | 678 |
Quantum yield | 0.261 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
iFluor® 660 succinimidyl ester |
iFluor® 660 amine |
Overview | ![]() ![]() |
Molecular weight 1117.16 | Correction Factor (260 nm) 0.07 | Correction Factor (280 nm) 0.08 | Extinction coefficient (cm -1 M -1) 2500001 | Excitation (nm) 663 | Emission (nm) 678 | Quantum yield 0.261 |
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 514, 555, 633, 647 and 808 nm). iFluor® 660 dyes have fluorescence excitation and emission maxima of ~660 nm and ~680 nm respectively. These spectral characteristics make them a unique acceptor for preparing PE, APC and PerCP tandems that are widely used in flow cytometry applications. iFluor® 660 Maleimide is stable and shows good reactivity and selectivity with protein thiol group.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 660 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 89.513 µL | 447.563 µL | 895.127 µL | 4.476 mL | 8.951 mL |
5 mM | 17.903 µL | 89.513 µL | 179.025 µL | 895.127 µL | 1.79 mL |
10 mM | 8.951 µL | 44.756 µL | 89.513 µL | 447.563 µL | 895.127 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Correction Factor (260 nm) | 0.07 |
Correction Factor (280 nm) | 0.08 |
Extinction coefficient (cm -1 M -1) | 2500001 |
Excitation (nm) | 663 |
Emission (nm) | 678 |
Quantum yield | 0.261 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 350 maleimide | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
iFluor® 488 maleimide | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 555 maleimide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 647 maleimide | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
iFluor® 680 maleimide | 684 | 701 | 2200001 | 0.231 | 0.097 | 0.094 |
iFluor® 700 maleimide | 690 | 713 | 2200001 | 0.231 | 0.09 | 0.04 |
iFluor® 750 maleimide | 757 | 779 | 2750001 | 0.121 | 0.044 | 0.039 |
iFluor® 790 maleimide | 787 | 812 | 2500001 | 0.131 | 0.1 | 0.09 |
iFluor® 800 maleimide | 801 | 820 | 2500001 | 0.111 | 0.03 | 0.08 |
Show More (20) |
Images

Figure 1. iFluor® 660 dyes have fluorescence excitation and emission maxima of ~660 nm and ~680 nm respectively. These spectral characteristics make them a unique acceptor for preparing PE, APC and PerCP tandems that are widely used in flow cytometry applications. iFluor® 660 Maleimide is stable and shows good reactivity and selectivity with protein thiol group.
References
View all 31 references: Citation Explorer
Abortive ligation intermediate blocks seamless repair of double-stranded breaks.
Authors: Li, Xuegang and Jin, Jiacheng and Xu, Wenxuan and Wang, Mingdao and Liu, Liangwei
Journal: International journal of biological macromolecules (2022): 1498-1503
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Journal: International journal of biological macromolecules (2022): 1498-1503
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Authors: Song, Dan and Liu, Jiayao and Xu, Wenjuan and Han, Xiangzhi and Wang, Hongliang and Zhuo, Yuxin and Li, Chunsheng and Long, Feng
Journal: Mikrochimica acta (2022): 234
Authors: Song, Dan and Liu, Jiayao and Xu, Wenjuan and Han, Xiangzhi and Wang, Hongliang and Zhuo, Yuxin and Li, Chunsheng and Long, Feng
Journal: Mikrochimica acta (2022): 234
Mechanism of Cyanine5 to Cyanine3 Photoconversion and Its Application for High-Density Single-Particle Tracking in a Living Cell.
Authors: Cho, Yoonjung and An, Hyeong Jeon and Kim, Taehoon and Lee, Chulbom and Lee, Nam Ki
Journal: Journal of the American Chemical Society (2021): 14125-14135
Authors: Cho, Yoonjung and An, Hyeong Jeon and Kim, Taehoon and Lee, Chulbom and Lee, Nam Ki
Journal: Journal of the American Chemical Society (2021): 14125-14135
Molecular and Spectroscopic Characterization of Green and Red Cyanine Fluorophores from the Alexa Fluor and AF Series*.
Authors: Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben
Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2021)
Authors: Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben
Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2021)
Restricted intramolecular rotation of fluorescent molecular rotors at the periphery of aqueous microdroplets in oil.
Authors: Kang, Jooyoun and Lhee, SangMoon and Lee, Jae Kyoo and Zare, Richard N and Nam, Hong Gil
Journal: Scientific reports (2020): 16859
Authors: Kang, Jooyoun and Lhee, SangMoon and Lee, Jae Kyoo and Zare, Richard N and Nam, Hong Gil
Journal: Scientific reports (2020): 16859
Development of sesbania mosaic virus nanoparticles for imaging.
Authors: Vishnu Vardhan, G P and Hema, M and Sushmitha, C and Savithri, H S and Natraj, Usha and Murthy, M R N
Journal: Archives of virology (2019): 497-507
Authors: Vishnu Vardhan, G P and Hema, M and Sushmitha, C and Savithri, H S and Natraj, Usha and Murthy, M R N
Journal: Archives of virology (2019): 497-507
PET imaging of EGFR expression using an 18F-labeled RNA aptamer.
Authors: Cheng, Siyuan and Jacobson, Orit and Zhu, Guizhi and Chen, Zhen and Liang, Steve H and Tian, Rui and Yang, Zhen and Niu, Gang and Zhu, Xiaohua and Chen, Xiaoyuan
Journal: European journal of nuclear medicine and molecular imaging (2019): 948-956
Authors: Cheng, Siyuan and Jacobson, Orit and Zhu, Guizhi and Chen, Zhen and Liang, Steve H and Tian, Rui and Yang, Zhen and Niu, Gang and Zhu, Xiaohua and Chen, Xiaoyuan
Journal: European journal of nuclear medicine and molecular imaging (2019): 948-956
Deciphering Design Principles of Förster Resonance Energy Transfer-Based Protease Substrates: Thermolysin-Like Protease from Geobacillus stearothermophilus as a Test Case.
Authors: Ripp, Sophie and Turunen, Petri and Minot, Ethan D and Rowan, Alan E and Blank, Kerstin G
Journal: ACS omega (2018): 4148-4156
Authors: Ripp, Sophie and Turunen, Petri and Minot, Ethan D and Rowan, Alan E and Blank, Kerstin G
Journal: ACS omega (2018): 4148-4156
Targetability of hyaluronic acid nanogel to cancer cells: In vitro and in vivo studies.
Authors: Pedrosa, S S and Pereira, P and Correia, A and Gama, F M
Journal: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences (2017): 102-113
Authors: Pedrosa, S S and Pereira, P and Correia, A and Gama, F M
Journal: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences (2017): 102-113
[Zinc-dependent metalloprotease 1 (Zmp1) inhibits the proliferation and phagosome-lysosome fusion of mouse macrophage RAW264.7].
Authors: Fang, Chencheng and Zhang, Jiming and Lu, Nan and Li, Peng and Fan, Yu and Kang, Yuexi and Yang, Chun and He, Yonglin
Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (2016): 1317-1320
Authors: Fang, Chencheng and Zhang, Jiming and Lu, Nan and Li, Peng and Fan, Yu and Kang, Yuexi and Yang, Chun and He, Yonglin
Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (2016): 1317-1320