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iFluor® 660 maleimide

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 514, 555, 633, 647 and 808 nm). iFluor® 660 dyes have fluorescence excitation and emission maxima of ~660 nm and ~680 nm respectively. These spectral characteristics make them a unique acceptor for preparing PE, APC and PerCP tandems that are widely used in flow cytometry applications. iFluor® 660 Maleimide is stable and shows good reactivity and selectivity with protein thiol group.

Fluorescent dye maleimides are the most popular tool for conjugating dyes to a peptide, protein, antibody, thiol-modified oligonucleotide or nucleic acid through their SH group. Maleimides react readily with the thiol group of proteins, thiol-modified oligonucleotides, and other thiol-containing molecules under neutral conditions. The resulting dye conjugates are quite stable. iFluor® 660 dyes have fluorescence excitation and emission maxima of ~660 nm and ~680 nm respectively. These spectral characteristics make them a unique acceptor for preparing PE, APC and PerCP tandems that are widely used in flow cytometry applications. iFluor® 660 maleimide is stable and shows good reactivity and selectivity with protein thiol groups.
Fluorescent dye maleimides are the most popular tool for conjugating dyes to a peptide, protein, antibody, thiol-modified oligonucleotide or nucleic acid through their SH group. Maleimides react readily with the thiol group of proteins, thiol-modified oligonucleotides, and other thiol-containing molecules under neutral conditions. The resulting dye conjugates are quite stable. iFluor® 660 dyes have fluorescence excitation and emission maxima of ~660 nm and ~680 nm respectively. These spectral characteristics make them a unique acceptor for preparing PE, APC and PerCP tandems that are widely used in flow cytometry applications. iFluor® 660 maleimide is stable and shows good reactivity and selectivity with protein thiol groups.
CatalogSize
Price
Quantity
725011 mg
Price
 
Physical properties

Molecular weight1117.16
SolventDMSO
Spectral properties

Correction factor (260 nm)0.07
Correction factor (280 nm)0.08
Extinction coefficient (cm -1 M -1)
250000
1
Excitation (nm)663
Emission (nm)678
Quantum yield
0.26
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Page updated on October 22, 2025