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iFluor® 350 maleimide
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 350 dyes have fluorescence excitation and emission maxima of ~345 nm and ~450 nm respectively. These spectral characteristics make them an excellent alternative to AMCA and Alexa Fluor® 350 labeling dye (Alexa Fluor® is the trademark of Invitrogen). iFluor® 350 maleimide is reasonably stable and shows good reactivity and selectivity with the thiol group.
<p>HeLa cells were stained with mouse anti-tubulin followed by iFluor 350 goat anti-mouse IgG (H+L).</p>
<p>HeLa cells were stained with mouse anti-tubulin followed by iFluor 350 goat anti-mouse IgG (H+L).</p>
CatalogSize
Price
Quantity
10601 mg
Price
 
Physical properties

Molecular weight355.34
SolventDMSO
Spectral properties

Correction factor (260 nm)0.83
Correction factor (280 nm)0.23
Extinction coefficient (cm -1 M -1)
20000
1
Excitation (nm)345
Emission (nm)450
Quantum yield
0.95
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 8, 2025