iFluor® 514 maleimide
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| Quotation | Request |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 950.64 |
| Solvent | DMSO |
Spectral properties
| Absorbance (nm) | 511 |
| Correction Factor (260 nm) | 0.265 |
| Correction Factor (280 nm) | 0.116 |
| Extinction coefficient (cm -1 M -1) | 750001 |
| Excitation (nm) | 511 |
| Emission (nm) | 527 |
| Quantum yield | 0.831 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
| iFluor® 514 succinimidyl ester |
| Overview |  SDSProtocol |
Molecular weight 950.64 | Absorbance (nm) 511 | Correction Factor (260 nm) 0.265 | Correction Factor (280 nm) 0.116 | Extinction coefficient (cm -1 M -1) 750001 | Excitation (nm) 511 | Emission (nm) 527 | Quantum yield 0.831 |
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 514, 555 and 633 nm). iFluor® 514 dyes have fluorescence excitation and emission maxima of ~510 nm and ~527 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 514 labeling dye and rhodamine 6G (Alexa Fluor® is the trademark of Invitrogen). For some antibody conjugates, iFluor® 514 conjugates are significantly brighter. iFluor® 514 Maleimide is stable and shows good reactivity and selectivity with protein thiol group.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 514 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 105.192 µL | 525.961 µL | 1.052 mL | 5.26 mL | 10.519 mL |
| 5 mM | 21.038 µL | 105.192 µL | 210.385 µL | 1.052 mL | 2.104 mL |
| 10 mM | 10.519 µL | 52.596 µL | 105.192 µL | 525.961 µL | 1.052 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Absorbance (nm) | 511 |
| Correction Factor (260 nm) | 0.265 |
| Correction Factor (280 nm) | 0.116 |
| Extinction coefficient (cm -1 M -1) | 750001 |
| Excitation (nm) | 511 |
| Emission (nm) | 527 |
| Quantum yield | 0.831 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| iFluor® 350 maleimide | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
| iFluor® 488 maleimide | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
| iFluor® 555 maleimide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| iFluor® 647 maleimide | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
| iFluor® 680 maleimide | 684 | 701 | 2200001 | 0.231 | 0.097 | 0.094 |
| iFluor® 700 maleimide | 690 | 713 | 2200001 | 0.231 | 0.09 | 0.04 |
| iFluor® 750 maleimide | 757 | 779 | 2750001 | 0.121 | 0.044 | 0.039 |
| iFluor® 790 maleimide | 787 | 812 | 2500001 | 0.131 | 0.1 | 0.09 |
| iFluor® 800 maleimide | 801 | 820 | 2500001 | 0.111 | 0.03 | 0.08 |
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Images
References
View all 3 references: Citation Explorer
Dual-axis confocal configuration for depth sensitive fluorescence spectroscopy.
Authors: Lin, Yongping and Chang, Tse-Shao and Chen, Jing and Li, Gaoming
Journal: Optics letters (2021): 3588-3591
Authors: Lin, Yongping and Chang, Tse-Shao and Chen, Jing and Li, Gaoming
Journal: Optics letters (2021): 3588-3591
Toxicity of organic fluorophores used in molecular imaging: literature review.
Authors: Alford, Raphael and Simpson, Haley M and Duberman, Josh and Hill, G Craig and Ogawa, Mikako and Regino, Celeste and Kobayashi, Hisataka and Choyke, Peter L
Journal: Molecular imaging (2009): 341-54
Authors: Alford, Raphael and Simpson, Haley M and Duberman, Josh and Hill, G Craig and Ogawa, Mikako and Regino, Celeste and Kobayashi, Hisataka and Choyke, Peter L
Journal: Molecular imaging (2009): 341-54
Single-molecule dynamics of phytochrome-bound fluorophores probed by fluorescence correlation spectroscopy.
Authors: Miller, Abigail E and Fischer, Amanda J and Laurence, Ted and Hollars, Christopher W and Saykally, Richard J and Lagarias, J Clark and Huser, Thomas
Journal: Proceedings of the National Academy of Sciences of the United States of America (2006): 11136-41
Authors: Miller, Abigail E and Fischer, Amanda J and Laurence, Ted and Hollars, Christopher W and Saykally, Richard J and Lagarias, J Clark and Huser, Thomas
Journal: Proceedings of the National Academy of Sciences of the United States of America (2006): 11136-41