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AAT Bioquest

iFluor® 514 maleimide

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 514, 555 and 633 nm). iFluor® 514 dyes have fluorescence excitation and emission maxima of ~510 nm and ~527 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 514 labeling dye and rhodamine 6G (Alexa Fluor® is the trademark of Invitrogen). For some antibody conjugates, iFluor® 514 conjugates are significantly brighter. iFluor® 514 Maleimide is stable and shows good reactivity and selectivity with protein thiol group.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 350 maleimide3454502000010.9510.830.23
iFluor® 488 maleimide4915167500010.910.210.11
iFluor® 555 maleimide55757010000010.6410.230.14
iFluor® 647 maleimide65667025000010.2510.030.03
iFluor® 680 maleimide68470122000010.2310.0970.094
iFluor® 700 maleimide69071322000010.2310.090.04
iFluor® 750 maleimide75777927500010.1210.0440.039
iFluor® 790 maleimide78781225000010.1310.10.09
iFluor® 800 maleimide80182025000010.1110.030.08
iFluor® 810 maleimide81182225000010.0510.090.15
iFluor® 820 maleimide82285025000010.110.16
iFluor® 860 maleimide85387825000010.10.14
iFluor® 532 maleimide5375609000010.6810.260.16
iFluor® 594 maleimide58760320000010.5310.050.04
iFluor® 405 maleimide4034273700010.9110.480.77
iFluor® 430 maleimide4334984000010.7810.680.3
iFluor® 568 maleimide56858710000010.5710.340.15
iFluor® 633 maleimide64065425000010.2910.0620.044
iFluor® 450 maleimide4515024000010.8210.450.27
iFluor® 460 maleimide468493800001~0.810.980.46
iFluor® 665 maleimide667692110,00010.2210.120.09
iFluor® 514 Styramide *Superior Replacement for Opal 540*5115277500010.8310.2650.116
iFluor® 546 maleimide54155710000010.6710.250.15
iFluor® 840 maleimide8368792000001-0.20.09
iFluor® 770 maleimide77779725000010.160.090.08
iFluor® 780 maleimide78480825000010.1610.130.12
iFluor® 830 maleimide830867----
iFluor® 660 maleimide66367825000010.2610.070.08
iFluor® 670 maleimide67168220000010.5510.030.033
iFluor® 720 maleimide71674024000010.1410.150.13
ATTO 514 maleimide510531115,0000.850.210.08
Show More (22)

References

View all 3 references: Citation Explorer
Dual-axis confocal configuration for depth sensitive fluorescence spectroscopy.
Authors: Lin, Yongping and Chang, Tse-Shao and Chen, Jing and Li, Gaoming
Journal: Optics letters (2021): 3588-3591
Toxicity of organic fluorophores used in molecular imaging: literature review.
Authors: Alford, Raphael and Simpson, Haley M and Duberman, Josh and Hill, G Craig and Ogawa, Mikako and Regino, Celeste and Kobayashi, Hisataka and Choyke, Peter L
Journal: Molecular imaging (2009): 341-54
Single-molecule dynamics of phytochrome-bound fluorophores probed by fluorescence correlation spectroscopy.
Authors: Miller, Abigail E and Fischer, Amanda J and Laurence, Ted and Hollars, Christopher W and Saykally, Richard J and Lagarias, J Clark and Huser, Thomas
Journal: Proceedings of the National Academy of Sciences of the United States of America (2006): 11136-41
Page updated on October 4, 2024

Ordering information

Price
Unit size
Catalog Number72500
Quantity
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Additional ordering information

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Fax1-800-609-2943
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Physical properties

Molecular weight

950.64

Solvent

DMSO

Spectral properties

Absorbance (nm)

511

Correction Factor (260 nm)

0.265

Correction Factor (280 nm)

0.116

Extinction coefficient (cm -1 M -1)

750001

Excitation (nm)

511

Emission (nm)

527

Quantum yield

0.831

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure