iFluor® 514 maleimide
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 514, 555 and 633 nm). iFluor® 514 dyes have fluorescence excitation and emission maxima of ~510 nm and ~527 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 514 labeling dye and rhodamine 6G (Alexa Fluor® is the trademark of Invitrogen). For some antibody conjugates, iFluor® 514 conjugates are significantly brighter. iFluor® 514 Maleimide is stable and shows good reactivity and selectivity with protein thiol group.
Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 350 maleimide | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
iFluor® 488 maleimide | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 555 maleimide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 647 maleimide | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
iFluor® 680 maleimide | 684 | 701 | 2200001 | 0.231 | 0.097 | 0.094 |
iFluor® 700 maleimide | 690 | 713 | 2200001 | 0.231 | 0.09 | 0.04 |
iFluor® 750 maleimide | 757 | 779 | 2750001 | 0.121 | 0.044 | 0.039 |
iFluor® 790 maleimide | 787 | 812 | 2500001 | 0.131 | 0.1 | 0.09 |
iFluor® 800 maleimide | 801 | 820 | 2500001 | 0.111 | 0.03 | 0.08 |
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References
View all 3 references: Citation Explorer
Dual-axis confocal configuration for depth sensitive fluorescence spectroscopy.
Authors: Lin, Yongping and Chang, Tse-Shao and Chen, Jing and Li, Gaoming
Journal: Optics letters (2021): 3588-3591
Authors: Lin, Yongping and Chang, Tse-Shao and Chen, Jing and Li, Gaoming
Journal: Optics letters (2021): 3588-3591
Toxicity of organic fluorophores used in molecular imaging: literature review.
Authors: Alford, Raphael and Simpson, Haley M and Duberman, Josh and Hill, G Craig and Ogawa, Mikako and Regino, Celeste and Kobayashi, Hisataka and Choyke, Peter L
Journal: Molecular imaging (2009): 341-54
Authors: Alford, Raphael and Simpson, Haley M and Duberman, Josh and Hill, G Craig and Ogawa, Mikako and Regino, Celeste and Kobayashi, Hisataka and Choyke, Peter L
Journal: Molecular imaging (2009): 341-54
Single-molecule dynamics of phytochrome-bound fluorophores probed by fluorescence correlation spectroscopy.
Authors: Miller, Abigail E and Fischer, Amanda J and Laurence, Ted and Hollars, Christopher W and Saykally, Richard J and Lagarias, J Clark and Huser, Thomas
Journal: Proceedings of the National Academy of Sciences of the United States of America (2006): 11136-41
Authors: Miller, Abigail E and Fischer, Amanda J and Laurence, Ted and Hollars, Christopher W and Saykally, Richard J and Lagarias, J Clark and Huser, Thomas
Journal: Proceedings of the National Academy of Sciences of the United States of America (2006): 11136-41
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