iFluor® 750 maleimide

iFluor® 750 maleimide is a near-infrared fluorescent, thiol-reactive dye designed for covalent labeling of proteins, particularly antibodies, and other sulfhydryl-containing biomolecules.

Thiol-Reactive Labeling: Exhibits good reactivity and selectivity toward protein thiol groups.
Spectral Properties: Excitation/emission maxima of 757/779 nm.
Photostability: Provides good photostability and greater brightness than comparable Cy7® and Alexa Fluor® 750 dyes.
Labeling Conditions: Supports protein conjugation at pH 6.5–7.0.

<p>HL-60 cells were incubated with (Red, +) or without (Green, -) Anti-human HLA-ABC (W6/32 mAb), followed by iFluor® 750 goat anti-mouse IgG conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC-Cy7 channel.</p>

Protocol

SDS

Catalog	Size	Price	Quantity
1068	1 mg	Price

Overview

AAT Bioquest's iFluor® dyes are developed for labeling proteins, particularly antibodies. These dyes are optimized to have minimal fluorescence quenching effect on proteins and nucleic acids. Our iFluor® 750 dyes have fluorescence excitation and emission maxima of ~757 nm and ~779 nm, respectively, with good photostability. Our in-house comparable studies indicated that our iFluor® 750 dyes are significantly brighter than the corresponding Cy7® and Alexa Fluor® 750. These spectral characteristics make them a superior alternative to Cy7® and Alexa Fluor® 750 (Cy7® and Alexa Fluor® are the trademarks of GE Healthcare and Invitrogen). iFluor® 780 conjugates have been widely used in fluorescence animal imaging applications. The iFluor® 750 maleimide is reasonably stable and shows good reactivity and selectivity with the thiol group.

Citations

View all 3 citations: Citation Explorer

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification

Authors:

Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others

Journal:

Metabolomics (Los Angel) (2017): 2153--0769

Suramin inhibits cullin-RING E3 ubiquitin ligases

Authors:

Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others

Journal:

Proceedings of the National Academy of Sciences (2016): E2011--E2018

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function

Authors:

Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra and Martens, Erik and Liekens, S and ra and Noppen, Sam and Damme, Jo Van and others

Journal:

International Journal of Cancer (2012): E425--E436

References

View all 49 references: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes

Authors:

Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.

Journal:

Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling

Authors:

Zhang S, Tan HC, Ooi EE.

Journal:

J Vis Exp. (2011)

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells

Authors:

Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.

Journal:

Biochem Biophys Res Commun (2011): 211

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Authors:

Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.

Journal:

Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers

Authors:

Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.

Journal:

J Microbiol Methods (2011): 119

Physical properties

Molecular weight	~1100
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.044
Correction factor (280 nm)	0.039
Correction factor (565 nm)	0.0250
Correction factor (650 nm)	0.1413
Extinction coefficient (cm ^-1 M ^-1)	275000 ¹
Excitation (nm)	757
Emission (nm)	779
Quantum yield	0.12 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on November 13, 2026