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iFluor® 750 maleimide
AAT Bioquest's iFluor® dyes are developed for labeling proteins, particularly antibodies. These dyes are optimized to have minimal fluorescence quenching effect on proteins and nucleic acids. Our iFluor® 750 dyes have fluorescence excitation and emission maxima of ~757 nm and ~779 nm, respectively, with good photostability. Our in-house comparable studies indicated that our iFluor® 750 dyes are significantly brighter than the corresponding Cy7® and Alexa Fluor® 750. These spectral characteristics make them a superior alternative to Cy7® and Alexa Fluor® 750 (Cy7® and Alexa Fluor® are the trademarks of GE Healthcare and Invitrogen). iFluor® 780 conjugates have been widely used in fluorescence animal imaging applications. The iFluor® 750 maleimide is reasonably stable and shows good reactivity and selectivity with the thiol group.
<p>HL-60 cells were incubated with (Red, +) or without (Green, -) Anti-human HLA-ABC (W6/32 mAb), followed by iFluor® 750 goat anti-mouse IgG conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC-Cy7 channel.</p>
<p>HL-60 cells were incubated with (Red, +) or without (Green, -) Anti-human HLA-ABC (W6/32 mAb), followed by iFluor® 750 goat anti-mouse IgG conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC-Cy7 channel.</p>
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
10681 mg
Price
 
Physical properties
Molecular weight~1100
SolventDMSO
Spectral properties
Correction factor (260 nm)0.044
Correction factor (280 nm)0.039
Correction factor (565 nm)0.0250
Correction factor (650 nm)0.1413
Extinction coefficient (cm -1 M -1)
275000
1
Excitation (nm)757
Emission (nm)779
Quantum yield
0.12
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 8, 2025