iFluor® 750 Styramide™

iFluor® 750 Styramide™ is a near-infrared fluorescent signal amplification reagent designed for sensitive detection of low-abundance targets in cells and tissues using HRP-mediated labeling.

Detection Principle: Uses HRP-catalyzed covalent deposition of fluorescent labels.
High Sensitivity: Generates 10–50 times stronger fluorescence signals than conventional tyramide reagents.
Instrument Compatibility: Compatible with Cy7 fluorescence microscope filter sets.
Applications: Suitable for immunocytochemistry, immunofluorescence, and immunohistochemistry.

Fluorescence images of HeLa cells labeled with Mouse anti-Tubulin primary antibody. Cells were then stained with a HRP-labeled Goat anti-Mouse IgG secondary antibody followed by iFluor® 750 Styramide™ (Left) or Alexa Fluor® 750 tyramide (Right), respectively. Fluorescence images were taken using the Cy7 filter set. iFluor® 750 Styramide™ shows significantly higher fluorescence intensity than Alexa Fluor® 750 tyramide if under the same exposure time.

Protocol

SDS

Catalog	Size	Price	Quantity
45065	100 Slides	Price

Overview

Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA™ utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA™ radicals have much higher reactivity than tyramide radicals, making the PSA™ system much faster, more robust and sensitive than the traditional TSA reagents. Compared to tyramide reagents, the Styramide™ conjugates have ability to label the target at higher efficiency and thus generate significantly higher fluorescence signal. Styramide™ conjugates also allow significantly less consumption of primary antibody compared to standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 750 Styramide is a superior replacement for Alexa Fluor 750 tyramide or other spectrally similar fluorescent tyramide conjugates or TSA reagents.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 750 Styramide™ to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	79.512 µL	397.561 µL	795.121 µL	3.976 mL	7.951 mL
5 mM	15.902 µL	79.512 µL	159.024 µL	795.121 µL	1.59 mL
10 mM	7.951 µL	39.756 µL	79.512 µL	397.561 µL	795.121 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1257.67 g/mol	=	mL	x	mM	=	mmol

Citations

View all 7 citations: Citation Explorer

Tissue Factor Expression Is Associated with Distinct Pattern of Immune-Cell Infiltration in the Ovarian Tumor Microenvironment

Authors:

Mohiuddin, TM and Binte Hafiz, Fatema and Sheng, Wenjie and Zhang, Chaoyu and Al-Rawe, Marwah and Zeppernick, Felix and Tchaikovski, Svetlana and Meinhold-Heerlein, Ivo and Hussain, Ahmad Fawzi

Journal:

International Journal of Molecular Sciences (2026): 9148

A Customizable Tyramide Signal Amplification-Based Multiplex Immunofluorescence Protocol for FFPE Tissues

Authors:

Sheng, Wenjie and Mohiuddin, TM and Zhang, Chaoyu and Al-Rawe, Marwah and Konrad, Lutz and Wagner, Steffen and Zeppernick, Felix and Meinhold-Heerlein, Ivo and Hussain, Ahmad Fawzi

Journal:

Current Issues in Molecular Biology (2026): 439

Multiplexed Immunofluorescent Characterization of T-Cell and Myeloid Cell Populations Reveals Histologic Subtype Differences Within the Immunosuppressive Landscape of Human Basal Cell Carcinoma

Authors:

Shen, Alan S and Heusinkveld, Lauren E and Zalavadia, Ajay and Branicky, Andrelie and Anand, Sanjay and Piliang, Melissa and Maytin, Edward V

Journal:

Journal of Cutaneous Pathology (2026)

Metformin Ameliorates Early Brain Injury After Subarachnoid Hemorrhage Via Improving Endoplasmic Reticulum Stress and Mitochondrial Stress-Mediated Ca2+ Imbalance

Authors:

Zhang, Zhonghua and Zhang, Jingbei and Li, Liping and Zong, Lin

Journal:

Molecular Neurobiology (2026): 256

Tyramide signal amplification-based detection system: A novel approach to improve detection efficiency for circulating tumor cells

Authors:

Chen, Lin and Yang, Zhonglin and Lu, Yue and Li, Shan and Tang, Dongjiang and Zhang, Lei

Journal:

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy (2025): 126898

Physical properties

Molecular weight	1257.67
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.044
Correction factor (280 nm)	0.039
Correction factor (565 nm)	0.0250
Correction factor (650 nm)	0.1413
Extinction coefficient (cm ^-1 M ^-1)	275000 ¹
Excitation (nm)	757
Emission (nm)	779
Quantum yield	0.12 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Fluorescence microscope
Excitation	Cy7 filter set
Emission	Cy7 filter set
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 30, 2026