Name: iFluor® 680 maleimide
Brand: AAT Bioquest
SKU: 1066
Price: 237 USD
Availability: InStock

iFluor® 680 maleimide

iFluor® 680 hydrazide is a far-red fluorescent, carbonyl-reactive dye designed for covalent labeling of aldehyde-containing biomolecules and other carbonyl-functionalized compounds.

Carbonyl-Reactive Labeling: Exhibits good reactivity and selectivity toward aldehyde groups.
Spectral Properties: Excitation/emission maxima of 684/701 nm.
Photostability: Provides bright, photostable fluorescence with minimal protein quenching.
Dye Compatibility: Spectrally similar to Cy5.5® and Alexa Fluor® 680.

HeLa cells were stained with mouse anti-tubulin followed with iFluor® 680 goat anti-mouse IgG (H+L); and nuclei were stained with Nuclear Green™ DCS1 (Cat# 17550).

Protocol

SDS

Catalog	Size	Price	Quantity
1066	1 mg	Price

Overview

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 680 dyes have fluorescence excitation and emission maxima of ~684 nm and ~701 nm respectively. These spectral characteristics make them an excellent alternative to Cy5.5® and Alexa Fluor® 680 labeling dyes (Cy5.5® and Alexa Fluor® are the trademarks of GE Health Care and Invitrogen). iFluor® 680 maleimide is reasonably stable and shows good reactivity and selectivity with the thiol group.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 680 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	100.497 µL	502.487 µL	1.005 mL	5.025 mL	10.05 mL
5 mM	20.099 µL	100.497 µL	200.995 µL	1.005 mL	2.01 mL
10 mM	10.05 µL	50.249 µL	100.497 µL	502.487 µL	1.005 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	995.05 g/mol	=	mL	x	mM	=	mmol

Citations

View all 3 citations: Citation Explorer

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification

Authors:

Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined

Journal:

Metabolomics (Los Angel) (2017): 2153--0769

Suramin inhibits cullin-RING E3 ubiquitin ligases

Authors:

Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined

Journal:

Proceedings of the National Academy of Sciences (2016): E2011--E2018

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function

Authors:

Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined

Journal:

International Journal of Cancer (2012): E425--E436

References

View all 49 references: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes

Authors:

Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.

Journal:

Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling

Authors:

Zhang S, Tan HC, Ooi EE.

Journal:

J Vis Exp. (2011)

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells

Authors:

Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.

Journal:

Biochem Biophys Res Commun (2011): 211

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Authors:

Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.

Journal:

Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers

Authors:

Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.

Journal:

J Microbiol Methods (2011): 119

Physical properties

Molecular weight	995.05
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.097
Correction factor (280 nm)	0.094
Extinction coefficient (cm ^-1 M ^-1)	220000 ¹
Excitation (nm)	684
Emission (nm)	701
Quantum yield	0.23 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 31, 2026