Name: iFluor® 594 maleimide
Brand: AAT Bioquest
SKU: 1064
Price: 237 USD
Availability: InStock

iFluor® 594 maleimide

iFluor® 594 maleimide is a thiol-reactive fluorescent dye designed for covalent labeling of proteins, particularly antibodies, through sulfhydryl groups.

Thiol-Reactive Labeling: Selectively conjugates to sulfhydryl groups, such as cysteine residues.
Spectral Properties: Excitation/emission maxima of 587/603 nm.
pH Stability: Exhibits pH-independent fluorescence from pH 3 to 11.
Dye Compatibility: Spectrally similar to Texas Red® and Alexa Fluor® 594.

<p>HeLa cells were stained with (Tubulin+) or without (Tubulin-) mouse anti-tubulin and then visualized with goat anti-mouse IgG - Alexa Fluor® 594 conjugate (Left) or goat anti-mouse IgG - iFluor® 594 (Right). Dye to protein ratio for each conjugate is in 2.8~3.0 range.</p>

Protocol

SDS

Catalog	Size	Price	Quantity
1064	1 mg	Price

Overview

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. iFluor® 594 dyes have fluorescence excitation and emission maxima of ~588 nm and ~604 nm respectively. iFluor® 594 family has spectral properties similar to those of Texas Red® and Alexa Fluor® 594 (Texas Red® and Alexa Fluor® 594 are the trademarks of Invitrogen). iFluor® 594 family is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family an excellent alternative to Texas Red® and Alexa Fluor® 594. Compared to Texas Red®, iFluor® 594 is much easier to conjugate with RPE with a much higher conjugation yield, and the resulting RPE-iFluor® 594 tandem has better FRET efficiency. iFluor® 555 maleimide is reasonably stable and shows good reactivity and selectivity with a thiol group.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 594 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	91.148 µL	455.739 µL	911.477 µL	4.557 mL	9.115 mL
5 mM	18.23 µL	91.148 µL	182.295 µL	911.477 µL	1.823 mL
10 mM	9.115 µL	45.574 µL	91.148 µL	455.739 µL	911.477 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1097.12 g/mol	=	mL	x	mM	=	mmol

Citations

View all 7 citations: Citation Explorer

CLEC3B (tetranectin) expression is associated with vascular localization and tumor aggressiveness in breast cancer

Authors:

Wei, Ching-Ting and Yu, Teng-Hung and Hsu, Chia-Chang and Hsuan, Chin-Feng and Hung, Wei-Chin and Wang, Chao-Ping and Tang, Wei-Hua and Chung, Fu-Mei and Lee, Yau-Jiunn and Chang, Chi-Chang and others

Journal:

International Journal of Medical Sciences (2026): 2715

Recombinant type I \& III chimeric collagen significantly enhances the vitality of fibroblasts and decelerates the skin aging process

Authors:

Chen, Wei and Wang, Ning and Xie, Xinshan and Liu, Han and Wang, Yiting and Nie, Yanfeng and Chen, Chun and Sun, Huaiqing and Hong, An and Guo, Chaowan and others

Journal:

BMC biotechnology (2025): 1--14

Endurance exercise upregulates mtp expression in aged Drosophila to ameliorate age-related diastolic dysfunction and extend lifespan

Authors:

Peng, Tianhang and Ding, Meng and Yan, Hanhui and Zhang, Ping and Tian, Rui and Guo, Yin and Zheng, Lan

Journal:

Physiological Reports (2024): e15929

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification

Authors:

Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others

Journal:

Metabolomics (Los Angel) (2017): 2153--0769

Suramin inhibits cullin-RING E3 ubiquitin ligases

Authors:

Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others

Journal:

Proceedings of the National Academy of Sciences (2016): E2011--E2018

References

View all 49 references: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes

Authors:

Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.

Journal:

Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling

Authors:

Zhang S, Tan HC, Ooi EE.

Journal:

J Vis Exp. (2011)

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells

Authors:

Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.

Journal:

Biochem Biophys Res Commun (2011): 211

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Authors:

Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.

Journal:

Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers

Authors:

Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.

Journal:

J Microbiol Methods (2011): 119

Physical properties

Molecular weight	1097.12
Solvent	DMSO

Spectral properties

Absorbance (nm)	587
Correction factor (260 nm)	0.05
Correction factor (280 nm)	0.04
Extinction coefficient (cm ^-1 M ^-1)	200000 ¹
Excitation (nm)	587
Emission (nm)	603
Quantum yield	0.53 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on November 14, 2026