iFluor® 625 maleimide
Ordering information
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Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1065.85 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
iFluor® 625 succinimidyl ester |
Overview | ![]() ![]() |
See also: iFluor® Dyes and Kits
Molecular weight 1065.85 |
iFluor® 625 maleimide selectively reacts with thiol group of a biomolecule. It is widely used to label reduced antibodies and other thiol-containing biomolecules such as peptides and thiol-modified oligos. iFluor®625 is a fluorophore from the iFluor family, which is known for its bright red fluorescence and compatibility with various fluorescence techniques and instruments. When excited with light in the red range (around 600 to 650 nm), iFluor 625 emits strong red fluorescence. It can also be well excited with red lasers at 633 and 647 nm. iFluor 625 can be conjugated to a variety of biomolecules, including antibodies, proteins, nucleic acids, and small molecules, enabling their visualization and tracking in cells and tissues. It is commonly used in fluorescence microscopy, immunohistochemistry, flow cytometry, and other fluorescence-based assays.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 625 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 93.822 µL | 469.109 µL | 938.218 µL | 4.691 mL | 9.382 mL |
5 mM | 18.764 µL | 93.822 µL | 187.644 µL | 938.218 µL | 1.876 mL |
10 mM | 9.382 µL | 46.911 µL | 93.822 µL | 469.109 µL | 938.218 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 350 maleimide | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
iFluor® 488 maleimide | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 555 maleimide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 647 maleimide | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
iFluor® 680 maleimide | 684 | 701 | 2200001 | 0.231 | 0.097 | 0.094 |
iFluor® 700 maleimide | 690 | 713 | 2200001 | 0.231 | 0.09 | 0.04 |
iFluor® 750 maleimide | 757 | 779 | 2750001 | 0.121 | 0.044 | 0.039 |
iFluor® 790 maleimide | 787 | 812 | 2500001 | 0.131 | 0.1 | 0.09 |
iFluor® 800 maleimide | 801 | 820 | 2500001 | 0.111 | 0.03 | 0.08 |
Show More (20) |
Images

Figure 1. Fluorescent dye maleimides are the most popular tool for conjugating dyes to a peptide, protein, antibody, thiol-modified oligonucleotide, or nucleic acid through their SH group. Maleimides react readily with the thiol group of proteins, thiol-modified oligonucleotides, and other thiol-containing molecules under neutral conditions. The resulting dye conjugates are quite stable.
References
View all 31 references: Citation Explorer
Design of the New Closo-Dodecarborate-Containing Gemcitabine Analogue for the Albumin-Based Theranostics Composition.
Authors: Raskolupova, Valeria I and Wang, Meiling and Dymova, Maya A and Petrov, Gleb O and Shchudlo, Ivan M and Taskaev, Sergey Yu and Abramova, Tatyana V and Godovikova, Tatyana S and Silnikov, Vladimir N and Popova, Tatyana V
Journal: Molecules (Basel, Switzerland) (2023)
Authors: Raskolupova, Valeria I and Wang, Meiling and Dymova, Maya A and Petrov, Gleb O and Shchudlo, Ivan M and Taskaev, Sergey Yu and Abramova, Tatyana V and Godovikova, Tatyana S and Silnikov, Vladimir N and Popova, Tatyana V
Journal: Molecules (Basel, Switzerland) (2023)
Sensitive and specific detection of breast cancer lymph node metastasis through dual-modality magnetic particle imaging and fluorescence molecular imaging: a preclinical evaluation.
Authors: Wang, Guorong and Li, Wenzhe and Shi, Guangyuan and Tian, Yu and Kong, Lingyan and Ding, Ning and Lei, Jing and Jin, Zhengyu and Tian, Jie and Du, Yang
Journal: European journal of nuclear medicine and molecular imaging (2022): 2723-2734
Authors: Wang, Guorong and Li, Wenzhe and Shi, Guangyuan and Tian, Yu and Kong, Lingyan and Ding, Ning and Lei, Jing and Jin, Zhengyu and Tian, Jie and Du, Yang
Journal: European journal of nuclear medicine and molecular imaging (2022): 2723-2734
Development and Characterization of a Novel Peptide-Drug Conjugate with DM1 for Treatment of FGFR2-Positive Tumors.
Authors: Wang, Yayu and Li, Yadan and Cao, Jieqiong and Meng, Qilin and Li, Xiaocen and Zhang, Yibo and Lam, Kit S and Hong, An and Liu, Ruiwu and Chen, Xiaojia
Journal: Biomedicines (2021)
Authors: Wang, Yayu and Li, Yadan and Cao, Jieqiong and Meng, Qilin and Li, Xiaocen and Zhang, Yibo and Lam, Kit S and Hong, An and Liu, Ruiwu and Chen, Xiaojia
Journal: Biomedicines (2021)
Molecular and Spectroscopic Characterization of Green and Red Cyanine Fluorophores from the Alexa Fluor and AF Series*.
Authors: Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben
Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2021)
Authors: Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben
Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2021)
Cyclic RGD-Functionalized closo-Dodecaborate Albumin Conjugates as Integrin Targeting Boron Carriers for Neutron Capture Therapy.
Authors: Kawai, Kazuki and Nishimura, Kai and Okada, Satoshi and Sato, Shinichi and Suzuki, Minoru and Takata, Takushi and Nakamura, Hiroyuki
Journal: Molecular pharmaceutics (2020): 3740-3747
Authors: Kawai, Kazuki and Nishimura, Kai and Okada, Satoshi and Sato, Shinichi and Suzuki, Minoru and Takata, Takushi and Nakamura, Hiroyuki
Journal: Molecular pharmaceutics (2020): 3740-3747
Virus-mimicking mesoporous organosilica nanocapsules with soft framework and rough surface for enhanced cellular uptake and tumor penetration.
Authors: Tao, Jun and Chen, Kun and Su, Xiaodan and Ren, Lili and Zhang, Junjie and Bao, Lei and Dong, Heng and Lu, Guangming and Teng, Zhaogang and Wang, Lianhui
Journal: Biomaterials science (2020): 2227-2233
Authors: Tao, Jun and Chen, Kun and Su, Xiaodan and Ren, Lili and Zhang, Junjie and Bao, Lei and Dong, Heng and Lu, Guangming and Teng, Zhaogang and Wang, Lianhui
Journal: Biomaterials science (2020): 2227-2233
Development of sesbania mosaic virus nanoparticles for imaging.
Authors: Vishnu Vardhan, G P and Hema, M and Sushmitha, C and Savithri, H S and Natraj, Usha and Murthy, M R N
Journal: Archives of virology (2019): 497-507
Authors: Vishnu Vardhan, G P and Hema, M and Sushmitha, C and Savithri, H S and Natraj, Usha and Murthy, M R N
Journal: Archives of virology (2019): 497-507
A Comparative Study on Albumin-Binding Molecules for Targeted Tumor Delivery through Covalent and Noncovalent Approach.
Authors: Um, Wooram and Park, Jooho and Youn, Ahye and Cho, Hanhee and Lim, Seungho and Lee, Jong Won and Yoon, Hong Yeol and Lim, Dong-Kwon and Park, Jae Hyung and Kim, Kwangmeyung
Journal: Bioconjugate chemistry (2019): 3107-3118
Authors: Um, Wooram and Park, Jooho and Youn, Ahye and Cho, Hanhee and Lim, Seungho and Lee, Jong Won and Yoon, Hong Yeol and Lim, Dong-Kwon and Park, Jae Hyung and Kim, Kwangmeyung
Journal: Bioconjugate chemistry (2019): 3107-3118
Facile preparation of near-infrared fluorescence and magnetic resonance dual-modality imaging probes based on mesoporous organosilica nanoparticles.
Authors: Li, Yanjiao and Guo, Wenwen and Su, Xiaodan and Lu, Nan and Wu, Guangyao and Ou-Yang, Lin and Dang, Meng and Tao, Jun and Teng, Zhaogang
Journal: Journal of colloid and interface science (2019): 277-286
Authors: Li, Yanjiao and Guo, Wenwen and Su, Xiaodan and Lu, Nan and Wu, Guangyao and Ou-Yang, Lin and Dang, Meng and Tao, Jun and Teng, Zhaogang
Journal: Journal of colloid and interface science (2019): 277-286
Surface Functionalization of Hepatitis E Virus Nanoparticles Using Chemical Conjugation Methods.
Authors: Chen, Chun Chieh and Stark, Marie and Baikoghli, Mo and Cheng, R Holland
Journal: Journal of visualized experiments : JoVE (2018)
Authors: Chen, Chun Chieh and Stark, Marie and Baikoghli, Mo and Cheng, R Holland
Journal: Journal of visualized experiments : JoVE (2018)
Application notes
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
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