iFluor® 625 maleimide

Ordering information

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	1065.85
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Alternative formats

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 625 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	93.822 µL	469.109 µL	938.218 µL	4.691 mL	9.382 mL
5 mM	18.764 µL	93.822 µL	187.644 µL	938.218 µL	1.876 mL
10 mM	9.382 µL	46.911 µL	93.822 µL	469.109 µL	938.218 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 350 maleimide	345	450	20000¹	0.95¹	0.83	0.23
iFluor® 488 maleimide	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 555 maleimide	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 647 maleimide	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 680 maleimide	684	701	220000¹	0.23¹	0.097	0.094
iFluor® 700 maleimide	690	713	220000¹	0.23¹	0.09	0.04
iFluor® 750 maleimide	757	779	275000¹	0.12¹	0.044	0.039
iFluor® 790 maleimide	787	812	250000¹	0.13¹	0.1	0.09
iFluor® 800 maleimide	801	820	250000¹	0.11¹	0.03	0.08
iFluor® 810 maleimide	811	822	250000¹	0.05¹	0.09	0.15
iFluor® 820 maleimide	822	850	250000¹		0.11	0.16
iFluor® 860 maleimide	853	878	250000¹		0.1	0.14
iFluor® 532 maleimide	537	560	90000¹	0.68¹	0.26	0.16
iFluor® 594 maleimide	588	604	180000¹	0.53¹	0.05	0.04
iFluor® 405 maleimide	403	427	37000¹	0.91¹	0.48	0.77
iFluor® 430 maleimide	433	498	40000¹	0.78¹	0.68	0.3
iFluor® 568 maleimide	568	587	100000¹	0.57¹	0.34	0.15
iFluor® 633 maleimide	640	654	250000¹	0.29¹	0.062	0.044
iFluor® 450 maleimide	451	502	40000¹	0.82¹	0.45	0.27
iFluor® 460 maleimide	468	493	80000¹	~0.8¹	0.98	0.46
iFluor® 665 maleimide	667	692	110,000¹	0.22¹	0.12	0.09
iFluor® 546 maleimide	541	557	100000¹	0.67¹	0.25	0.15
iFluor® 840 maleimide	836	879	200000¹	-	0.2	0.09
iFluor® 770 maleimide	777	797	250000¹	0.16	0.09	0.08
iFluor® 780 maleimide	784	808	250000¹	0.16¹	0.13	0.12
iFluor® 830 maleimide	830	867	-	-	-	-
iFluor® 514 maleimide	511	527	75000¹	0.83¹	0.265	0.116
iFluor® 660 maleimide	663	678	250000¹	0.26¹	0.07	0.08
iFluor® 670 maleimide	671	682	200000¹	0.55¹	0.03	0.033
Show More (20)

Images

Figure 1. Fluorescent dye maleimides are the most popular tool for conjugating dyes to a peptide, protein, antibody, thiol-modified oligonucleotide, or nucleic acid through their SH group. Maleimides react readily with the thiol group of proteins, thiol-modified oligonucleotides, and other thiol-containing molecules under neutral conditions. The resulting dye conjugates are quite stable.

References

View all 31 references: Citation Explorer

Design of the New Closo-Dodecarborate-Containing Gemcitabine Analogue for the Albumin-Based Theranostics Composition.
Authors: Raskolupova, Valeria I and Wang, Meiling and Dymova, Maya A and Petrov, Gleb O and Shchudlo, Ivan M and Taskaev, Sergey Yu and Abramova, Tatyana V and Godovikova, Tatyana S and Silnikov, Vladimir N and Popova, Tatyana V
Journal: Molecules (Basel, Switzerland) (2023)

Sensitive and specific detection of breast cancer lymph node metastasis through dual-modality magnetic particle imaging and fluorescence molecular imaging: a preclinical evaluation.
Authors: Wang, Guorong and Li, Wenzhe and Shi, Guangyuan and Tian, Yu and Kong, Lingyan and Ding, Ning and Lei, Jing and Jin, Zhengyu and Tian, Jie and Du, Yang
Journal: European journal of nuclear medicine and molecular imaging (2022): 2723-2734

Development and Characterization of a Novel Peptide-Drug Conjugate with DM1 for Treatment of FGFR2-Positive Tumors.
Authors: Wang, Yayu and Li, Yadan and Cao, Jieqiong and Meng, Qilin and Li, Xiaocen and Zhang, Yibo and Lam, Kit S and Hong, An and Liu, Ruiwu and Chen, Xiaojia
Journal: Biomedicines (2021)

Molecular and Spectroscopic Characterization of Green and Red Cyanine Fluorophores from the Alexa Fluor and AF Series*.
Authors: Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben
Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2021)

Cyclic RGD-Functionalized closo-Dodecaborate Albumin Conjugates as Integrin Targeting Boron Carriers for Neutron Capture Therapy.
Authors: Kawai, Kazuki and Nishimura, Kai and Okada, Satoshi and Sato, Shinichi and Suzuki, Minoru and Takata, Takushi and Nakamura, Hiroyuki
Journal: Molecular pharmaceutics (2020): 3740-3747

Virus-mimicking mesoporous organosilica nanocapsules with soft framework and rough surface for enhanced cellular uptake and tumor penetration.
Authors: Tao, Jun and Chen, Kun and Su, Xiaodan and Ren, Lili and Zhang, Junjie and Bao, Lei and Dong, Heng and Lu, Guangming and Teng, Zhaogang and Wang, Lianhui
Journal: Biomaterials science (2020): 2227-2233

Development of sesbania mosaic virus nanoparticles for imaging.
Authors: Vishnu Vardhan, G P and Hema, M and Sushmitha, C and Savithri, H S and Natraj, Usha and Murthy, M R N
Journal: Archives of virology (2019): 497-507

A Comparative Study on Albumin-Binding Molecules for Targeted Tumor Delivery through Covalent and Noncovalent Approach.
Authors: Um, Wooram and Park, Jooho and Youn, Ahye and Cho, Hanhee and Lim, Seungho and Lee, Jong Won and Yoon, Hong Yeol and Lim, Dong-Kwon and Park, Jae Hyung and Kim, Kwangmeyung
Journal: Bioconjugate chemistry (2019): 3107-3118

Facile preparation of near-infrared fluorescence and magnetic resonance dual-modality imaging probes based on mesoporous organosilica nanoparticles.
Authors: Li, Yanjiao and Guo, Wenwen and Su, Xiaodan and Lu, Nan and Wu, Guangyao and Ou-Yang, Lin and Dang, Meng and Tao, Jun and Teng, Zhaogang
Journal: Journal of colloid and interface science (2019): 277-286

Surface Functionalization of Hepatitis E Virus Nanoparticles Using Chemical Conjugation Methods.
Authors: Chen, Chun Chieh and Stark, Marie and Baikoghli, Mo and Cheng, R Holland
Journal: Journal of visualized experiments : JoVE (2018)