Name: iFluor® 810 maleimide
Brand: AAT Bioquest
SKU: 1388
Price: 355 USD
Availability: InStock

iFluor® 810 maleimide

iFluor® 810 maleimide is a near-infrared fluorescent, thiol-reactive dye designed for covalent labeling of proteins, antibodies, and other sulfhydryl-containing biomolecules.

Thiol-Reactive Labeling: Reacts with sulfhydryl groups to form stable conjugates.
Spectral Properties: Excitation/emission maxima of 811/822 nm.
Spectral Compatibility: Emission is well separated from Cy5, Cy7, and APC fluorophores.
Applications: Suitable for near-infrared small-animal imaging and multicolor fluorescence analysis.

<p>Fluorescent dye maleimides are the most popular tool for conjugating dyes to a peptide, protein, antibody, thiol-modified oligonucleotide or nucleic acid through their SH group. Maleimides react readily with the thiol group of proteins, thiol-modified oligonucleotides, and other thiol-containing molecules under neutral conditions. The resulting dye conjugates are quite stable.</p>

Protocol

SDS

Catalog	Size	Price	Quantity
1388	1 mg	Price

Overview

In vivo fluorescence imaging uses a sensitive camera to detect the fluorescence emission from fluorophores in whole-body living small animals. To overcome the photon attenuation in living tissue, fluorophores with long emission at the infrared (IR) region are generally preferred. Recent advances in imaging strategies and reporter techniques for in vivo fluorescence imaging include novel approaches to improve the specificity and affinity of the probes and to modulate and amplify the signal at target sites for enhanced sensitivity. Further emerging developments aim to achieve high-resolution, multimodality, and lifetime-based in vivo fluorescence imaging. Our iFluor® 810 is designed to label proteins and other biomolecules with infrared fluorescence. Conjugates prepared with iFluor® 810 have excitation and emission in the IR range. iFluor® 810 dye emission is well separated from commonly used far-red fluorophores such as Cy5, Cy7, or allophycocyanin (APC), facilitating multicolor analysis. This fluorophore is also useful for small animal in vivo imaging applications or other imaging applications requiring IR detections. iFluor® 810 maleimide is thiol-reactive and can be readily used to conjugate thiol-containing biomolecules.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 810 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	65.55 µL	327.751 µL	655.501 µL	3.278 mL	6.555 mL
5 mM	13.11 µL	65.55 µL	131.1 µL	655.501 µL	1.311 mL
10 mM	6.555 µL	32.775 µL	65.55 µL	327.751 µL	655.501 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1525.55 g/mol	=	mL	x	mM	=	mmol

Citations

View all 3 citations: Citation Explorer

Tumor cytotoxicity and immunogenicity of a novel V-jet neon plasma source compared to the kInpen

Authors:

Miebach, Lea and Freund, Eric and Horn, Stefan and Niessner, Felix and Sagwal, Sanjeev Kumar and von Woedtke, Thomas and Emmert, Steffen and Weltmann, Klaus-Dieter and Clemen, Ramona and Schmidt, Anke and others

Journal:

Scientific Reports (2021): 1-14

Tumor cytotoxicity and immunogenicity of a novel V-jet neon plasma source compared to the kINPen

Authors:

Journal:

Scientific Reports (2021): 1--14

Nanovesicle delivery to the liver via retinol binding protein and platelet-derived growth factor receptors: how targeting ligands affect biodistribution

Authors:

Hsu, Ching-Yun and Chen, Chun-Han and Aljuffali, Ibrahim A and Dai, You-Shan and Fang, Jia-You

Journal:

Nanomedicine (2017)

References

View all 18 references: Citation Explorer

A target cell-specific activatable fluorescence probe for in vivo molecular imaging of cancer based on a self-quenched avidin-rhodamine conjugate

Authors:

Hama Y, Urano Y, Koyama Y, Kamiya M, Bernardo M, Paik RS, Shin IS, Paik CH, Choyke PL, Kobayashi H.

Journal:

Cancer Res (2007): 2791

Fluorescence imaging in vivo: recent advances

Authors:

Rao J, Dragulescu-Andrasi A, Yao H.

Journal:

Curr Opin Biotechnol (2007): 17

Ex vivo fluorescence imaging of normal and malignant urothelial cells to enhance early diagnosis

Authors:

Steenkeste K, Lecart S, Deniset A, Pernot P, Eschwege P, Ferlicot S, Leveque-Fort S, Bri and et R, Fontaine-Aupart MP.

Journal:

Photochem Photobiol (2007): 1157

In vivo monitoring the fate of Cy5.5-Tat labeled T lymphocytes by quantitative near-infrared fluorescence imaging during acute brain inflammation in a rat model of experimental autoimmune encephalomyelitis

Authors:

Berger C, Gremlich HU, Schmidt P, Cannet C, Kneuer R, Hiest and P, Rausch M, Rudin M.

Journal:

J Immunol Methods (2007): 65

A protocol for imaging alternative splicing regulation in vivo using fluorescence reporters in transgenic mice

Authors:

Bonano VI, Oltean S, Garcia-Blanco MA.

Journal:

Nat Protoc (2007): 2166

Physical properties

Molecular weight	1525.55
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.09
Correction factor (280 nm)	0.15
Extinction coefficient (cm ^-1 M ^-1)	250000 ¹
Excitation (nm)	811
Emission (nm)	822
Quantum yield	0.05 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on November 12, 2026