iFluor® 830 maleimide
Ordering information
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Catalog Number | |
Unit Size | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1603.65 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 830 |
Excitation (nm) | 830 |
Emission (nm) | 867 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
iFluor® 830 succinimidyl ester |
iFluor® 830 acid |
Overview | ![]() ![]() |
See also: iFluor® Dyes and Kits
Molecular weight 1603.65 | Absorbance (nm) 830 | Excitation (nm) 830 | Emission (nm) 867 |
In vivo fluorescence imaging uses a sensitive camera to detect the fluorescence emission from fluorophores in whole-body living small animals. To overcome the photon attenuation in living tissue, fluorophores with long emissions at the infrared (IR) region are generally preferred. Recent advances in imaging strategies and reporter techniques for in vivo fluorescence imaging include novel approaches to improve the specificity and affinity of the probes and to modulate and amplify the signal at target sites for enhanced sensitivity. Further developments aim to achieve high-resolution, multimodality, and lifetime-based in vivo fluorescence imaging. Our iFluor® 830 is designed to label proteins and other biomolecules with infrared fluorescence. Conjugates prepared with iFluor® 830 have excitation and emission maxima in the IR range. iFluor® 830 dye emission is a unique color for spectrum flow cytometry as it is well separated from commonly used far-red fluorophores such as Cy5, Cy7, or allophycocyanin (APC), facilitating multicolor analysis. This fluorophore is also useful for small animal in vivo imaging applications or other imaging applications requiring IR detection. iFluor® 830 maleimide is one of the most convenient reactive forms for preparing the desired iFluor® 830 conjugates. It specifically and readily reacts with thiol groups, such as reduced antibodies and thiol-modified oligos.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 830 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 62.358 µL | 311.789 µL | 623.577 µL | 3.118 mL | 6.236 mL |
5 mM | 12.472 µL | 62.358 µL | 124.715 µL | 623.577 µL | 1.247 mL |
10 mM | 6.236 µL | 31.179 µL | 62.358 µL | 311.789 µL | 623.577 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Absorbance (nm) | 830 |
Excitation (nm) | 830 |
Emission (nm) | 867 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 350 maleimide | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
iFluor® 488 maleimide | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 555 maleimide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 647 maleimide | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
iFluor® 680 maleimide | 684 | 701 | 2200001 | 0.231 | 0.097 | 0.094 |
iFluor® 700 maleimide | 690 | 713 | 2200001 | 0.231 | 0.09 | 0.04 |
iFluor® 750 maleimide | 757 | 779 | 2750001 | 0.121 | 0.044 | 0.039 |
iFluor® 790 maleimide | 787 | 812 | 2500001 | 0.131 | 0.1 | 0.09 |
iFluor® 800 maleimide | 801 | 820 | 2500001 | 0.111 | 0.03 | 0.08 |
Show More (19) |
Images

Figure 1. iFluor® 830 is designed to label proteins and other biomolecules with infrared fluorescence. Conjugates prepared with iFluor® 830 have excitation and emission maxima in the IR range. iFluor® 830 maleimide is one of the most convenient reactive forms for preparing the desired iFluor® 830 conjugates as it specifically and readily reacts with thiol groups such as reduced antibodies and thiol-modified oligos.
References
View all 50 references: Citation Explorer
A bone-targeting drug delivery vehicle of a metal-organic framework conjugate with zoledronate combined with photothermal therapy for tumor inhibition in cancer bone metastasis.
Authors: Ge, Ting and Weiwei, Zhang and Ge, Fei and Zhu, Longbao and Song, Ping and Li, Wanzheng and Gui, Lin and Dong, Wan and Tao, Yugui and Yang, Kai
Journal: Biomaterials science (2022): 1831-1843
Authors: Ge, Ting and Weiwei, Zhang and Ge, Fei and Zhu, Longbao and Song, Ping and Li, Wanzheng and Gui, Lin and Dong, Wan and Tao, Yugui and Yang, Kai
Journal: Biomaterials science (2022): 1831-1843
Synthesis of sialic acid conjugates of the clinical near-infrared dye as next-generation theranostics for cancer phototherapy.
Authors: Dong, Huiling and Gao, Yanan and Huang, Xuefei and Wu, Xuanjun
Journal: Journal of materials chemistry. B (2022): 927-934
Authors: Dong, Huiling and Gao, Yanan and Huang, Xuefei and Wu, Xuanjun
Journal: Journal of materials chemistry. B (2022): 927-934
6-Aminocaproic acid as a linker to improve near-infrared fluorescence imaging and photothermal cancer therapy of PEGylated indocyanine green.
Authors: Hu, Qiang and Wang, Kesi and Qiu, Liyan
Journal: Colloids and surfaces. B, Biointerfaces (2021): 111372
Authors: Hu, Qiang and Wang, Kesi and Qiu, Liyan
Journal: Colloids and surfaces. B, Biointerfaces (2021): 111372
Diagnostic imaging in near-infrared photoimmunotherapy using a commercially available camera for indocyanine green.
Authors: Inagaki, Fuyuki F and Fujimura, Daiki and Furusawa, Aki and Okada, Ryuhei and Wakiyama, Hiroaki and Kato, Takuya and Choyke, Peter L and Kobayashi, Hisataka
Journal: Cancer science (2021): 1326-1330
Authors: Inagaki, Fuyuki F and Fujimura, Daiki and Furusawa, Aki and Okada, Ryuhei and Wakiyama, Hiroaki and Kato, Takuya and Choyke, Peter L and Kobayashi, Hisataka
Journal: Cancer science (2021): 1326-1330
Fluorescence Imaging of Tumor-Accumulating Antibody-IR700 Conjugates Prior to Near-Infrared Photoimmunotherapy (NIR-PIT) Using a Commercially Available Camera Designed for Indocyanine Green.
Authors: Inagaki, Fuyuki F and Fujimura, Daiki and Furusawa, Aki and Okada, Ryuhei and Wakiyama, Hiroaki and Kato, Takuya and Choyke, Peter L and Kobayashi, Hisataka
Journal: Molecular pharmaceutics (2021): 1238-1246
Authors: Inagaki, Fuyuki F and Fujimura, Daiki and Furusawa, Aki and Okada, Ryuhei and Wakiyama, Hiroaki and Kato, Takuya and Choyke, Peter L and Kobayashi, Hisataka
Journal: Molecular pharmaceutics (2021): 1238-1246
Hepatic bile acid transport increases in the postprandial state: A functional 11C-CSar PET/CT study in healthy humans.
Authors: Ørntoft, Nikolaj W and Gormsen, Lars C and Keiding, Susanne and Munk, Ole L and Ott, Peter and Sørensen, Michael
Journal: JHEP reports : innovation in hepatology (2021): 100288
Authors: Ørntoft, Nikolaj W and Gormsen, Lars C and Keiding, Susanne and Munk, Ole L and Ott, Peter and Sørensen, Michael
Journal: JHEP reports : innovation in hepatology (2021): 100288
Doxorubicin-Near infrared dye conjugate induces immunogenic cell death to enhance cancer immunotherapy.
Authors: Jin, Chan and Wang, Yan and Li, Yanfeng and Li, Jinbo and Zhou, Shuang and Yu, Jiang and Wang, Zhaomeng and Yu, Yueyang and Zhang, Haotian and Wang, Dun and He, Zhonggui and Wang, Yongjun
Journal: International journal of pharmaceutics (2021): 121027
Authors: Jin, Chan and Wang, Yan and Li, Yanfeng and Li, Jinbo and Zhou, Shuang and Yu, Jiang and Wang, Zhaomeng and Yu, Yueyang and Zhang, Haotian and Wang, Dun and He, Zhonggui and Wang, Yongjun
Journal: International journal of pharmaceutics (2021): 121027
Dual-colour (near-infrared/visible) emitting annexin V for fluorescence imaging of tumour cell apoptosis in vitro and in vivo.
Authors: Tsuboi, Setsuko and Jin, Takashi
Journal: RSC advances (2020): 38244-38250
Authors: Tsuboi, Setsuko and Jin, Takashi
Journal: RSC advances (2020): 38244-38250
Noncovalent Indocyanine Green Conjugate of C-Phycocyanin: Preparation and Tumor-Associated Macrophages-Targeted Photothermal Therapeutics.
Authors: Wan, Dong-Hua and Ma, Xin-Yue and Lin, Chen and Zhu, Deng-Hui and Li, Xingshu and Zheng, Bi-Yuan and Li, Jinyu and Ke, Mei-Rong and Huang, Jian-Dong
Journal: Bioconjugate chemistry (2020): 1438-1448
Authors: Wan, Dong-Hua and Ma, Xin-Yue and Lin, Chen and Zhu, Deng-Hui and Li, Xingshu and Zheng, Bi-Yuan and Li, Jinyu and Ke, Mei-Rong and Huang, Jian-Dong
Journal: Bioconjugate chemistry (2020): 1438-1448
Selenium and dopamine-crosslinked hyaluronic acid hydrogel for chemophotothermal cancer therapy.
Authors: Yang, Mingyu and Lee, Song Yi and Kim, Sungyun and Koo, Ja Seong and Seo, Ji-Hye and Jeong, Da In and Hwang, ChaeRim and Lee, Junmin and Cho, Hyun-Jong
Journal: Journal of controlled release : official journal of the Controlled Release Society (2020): 750-764
Authors: Yang, Mingyu and Lee, Song Yi and Kim, Sungyun and Koo, Ja Seong and Seo, Ji-Hye and Jeong, Da In and Hwang, ChaeRim and Lee, Junmin and Cho, Hyun-Jong
Journal: Journal of controlled release : official journal of the Controlled Release Society (2020): 750-764
Application notes
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
FAQ
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?