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iFluor® 830 succinimidyl ester

 iFluor® 830 is designed to label proteins and other biomolecules with infrared fluorescence. Conjugates prepared with iFluor® 830 have excitation and emission maxima in the IR range. iFluor® 830 succinimidyl ester is the most convenient reactive form for preparing the desired iFluor® 830 conjugates as it specifically and readily reacts with amino groups.
 iFluor® 830 is designed to label proteins and other biomolecules with infrared fluorescence. Conjugates prepared with iFluor® 830 have excitation and emission maxima in the IR range. iFluor® 830 succinimidyl ester is the most convenient reactive form for preparing the desired iFluor® 830 conjugates as it specifically and readily reacts with amino groups.
Ordering information
Price ()
Catalog Number1384
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight1527.88
Spectral properties
Excitation (nm)830
Emission (nm)867
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

OverviewpdfSDSpdfProtocol


Molecular weight
1527.88
Excitation (nm)
830
Emission (nm)
867
Our iFluor® 830 is designed to label proteins and other biomolecules with infrared fluorescence. Conjugates prepared with iFluor® 830 have excitation and emission maxima in the IR range. iFluor® 830 succinimidyl ester is the most convenient reactive form for preparing the desired iFluor® 830 conjugates as it specifically and readily reacts with amino groups. iFluor® 830 dye emission is a unique color for spectrum flow cytometry as it is well separated from commonly used far-red fluorophores such as Cy5, Cy7, or allophycocyanin (APC), facilitating multicolor analysis. This fluorophore is also useful for small animal in-vivo imaging applications or other imaging applications requiring IR detections. In vivo fluorescence imaging uses a sensitive camera to detect the fluorescence emission from fluorophores in whole-body living small animals. Fluorophores with long emissions at the infrared (IR) region are generally preferred to overcome photon attenuation in living tissue. Recent advances in imaging strategies and reporter techniques for in vivo fluorescence imaging include novel approaches to improve the specificity and affinity of the probes and to modulate and amplify the signal at target sites for enhanced sensitivity. Further developments aim to achieve high-resolution, multimodality, and lifetime-based in vivo fluorescence imaging.

Calculators


Common stock solution preparation

Table 1. Volume of appropriate solvent needed to reconstitute specific mass of iFluor® 830 succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM65.45 µL327.251 µL654.502 µL3.273 mL6.545 mL
5 mM13.09 µL65.45 µL130.9 µL654.502 µL1.309 mL
10 mM6.545 µL32.725 µL65.45 µL327.251 µL654.502 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Excitation (nm)830
Emission (nm)867

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 350 succinimidyl ester3454502000010.9510.830.23
iFluor® 405 succinimidyl ester4034273700010.9110.480.77
iFluor® 430 succinimidyl ester4334984000010.7810.680.3
iFluor® 440 succinimidyl ester4344804000010.6710.3520.229
iFluor® 450 succinimidyl ester4515024000010.8210.450.27
iFluor® 460 succinimidyl ester468493800001~0.810.980.46
iFluor® 488 succinimidyl ester4915167500010.910.210.11
iFluor® 514 succinimidyl ester5115277500010.8310.2650.116
iFluor® 532 succinimidyl ester5375609000010.6810.260.16
iFluor® 546 succinimidyl ester54155710000010.6710.250.15
iFluor® 555 succinimidyl ester55757010000010.6410.230.14
iFluor® 560 succinimidyl ester56057112000010.5710.04820.069
iFluor® 568 succinimidyl ester56858710000010.5710.340.15
iFluor® 570 succinimidyl ester56057112000010.5810.0480.069
iFluor® 594 succinimidyl ester58860418000010.5310.050.04
iFluor® 597 succinimidyl ester59861810000010.710.3350.514
iFluor® 610 succinimidyl ester61062811000010.8510.320.49
iFluor® 633 succinimidyl ester64065425000010.2910.0620.044
iFluor® 647 succinimidyl ester65667025000010.2510.030.03
iFluor® 660 succinimidyl ester66367825000010.2610.070.08
iFluor® 665 succinimidyl ester667692110,00010.2210.120.09
iFluor® 670 succinimidyl ester67168220000010.5510.030.033
iFluor® 680 succinimidyl ester68470122000010.2310.0970.094
iFluor® 690 succinimidyl ester68570422000010.3010.090.06
iFluor® 700 succinimidyl ester69071322000010.2310.090.04
iFluor® 710 succinimidyl ester71773919000010.6010.120.07
iFluor® 720 succinimidyl ester71674024000010.1410.150.13
iFluor® 740 succinimidyl ester74276422500010.2010.160.16
iFluor® 750 succinimidyl ester75777927500010.1210.0440.039
iFluor® 770 succinimidyl ester77779725000010.160.090.08
iFluor® 780 succinimidyl ester78480825000010.1610.130.12
iFluor® 790 succinimidyl ester78781225000010.1310.10.09
iFluor® 800 succinimidyl ester80182025000010.1110.030.08
iFluor® 810 succinimidyl ester81182225000010.0510.090.15
iFluor® 820 succinimidyl ester8228502500001-0.110.16
iFluor® 840 succinimidyl ester8368792000001-0.20.09
iFluor® 860 succinimidyl ester8538782500001-0.10.14
Show More (38)

References


View all 15 references: Citation Explorer
Deep learning for in vivo near-infrared imaging.
Authors: Ma, Zhuoran and Wang, Feifei and Wang, Weizhi and Zhong, Yeteng and Dai, Hongjie
Journal: Proceedings of the National Academy of Sciences of the United States of America (2021)
Measuring Kidney Perfusion, pH, and Renal Clearance Consecutively Using MRI and Multispectral Optoacoustic Tomography.
Authors: Minhas, Atul S and Sharkey, Jack and Randtke, Edward A and Murray, Patricia and Wilm, Bettina and Pagel, Mark D and Poptani, Harish
Journal: Molecular imaging and biology (2020): 494-503
Fast DNA Extraction with Polyacrylamide Microspheres for Polymerase Chain Reaction Detection.
Authors: Wang, Jun and Zhang, Shuyan and Xu, Xinhui and Xing, Yujun and Li, Zongru and Wang, Jinke
Journal: ACS omega (2020): 13829-13839
Evaluation of Targeting Efficiency of Joints with Anticollagen II Antibodies.
Authors: Lofchy, Laren A and Vu, Vivian P and Banda, Nirmal K and Ramirez, Joseline Ramos and Smith, Weston J and Gifford, Geoffrey and Gaikwad, Hanmant and Scheinman, Robert I and Simberg, Dmitri
Journal: Molecular pharmaceutics (2019): 2445-2451
A Modular Dual-Labeling Scaffold That Retains Agonistic Properties for Somatostatin Receptor Targeting.
Authors: Ghosh, Sukhen C and Rodriguez, Melissa and Carmon, Kendra S and Voss, Julie and Wilganowski, Nathaniel L and Schonbrunn, Agnes and Azhdarinia, Ali
Journal: Journal of nuclear medicine : official publication, Society of Nuclear Medicine (2017): 1858-1864
Longitudinal monitoring of skin accumulation of nanocarriers and biologicals with fiber optic near infrared fluorescence spectroscopy (FONIRS).
Authors: Griffin, James I and Benchimol, Michael J and Simberg, Dmitri
Journal: Journal of controlled release : official journal of the Controlled Release Society (2017): 167-174
Pharmacokinetic and Biodistribution Studies of HPMA Copolymer Conjugates in an Aseptic Implant Loosening Mouse Model.
Authors: Wei, Xin and Li, Fei and Zhao, Gang and Chhonker, Yashpal Singh and Averill, Christine and Galdamez, Josselyn and Purdue, P Edward and Wang, Xiaoyan and Fehringer, Edward V and Garvin, Kevin L and Goldring, Steven R and Alnouti, Yazen and Wang, Dong
Journal: Molecular pharmaceutics (2017): 1418-1428
Influence of chelator and near-infrared dye labeling on biocharacteristics of dual-labeled trastuzumab-based imaging agents.
Authors: Wang, Xuejuan and Aldrich, Melissa B and Yang, Zhi and Zhou, Nina and Xie, Qing and Liu, Chen and Sevick-Muraca, Eva
Journal: Chinese journal of cancer research = Chung-kuo yen cheng yen chiu (2016): 362-9
Preclinical characterization and validation of a dual-labeled trastuzumab-based imaging agent for diagnosing breast cancer.
Authors: Wang, Xuejuan and Aldrich, Melissa B and Marshall, Milton V and Sevick-Muraca, Eva M
Journal: Chinese journal of cancer research = Chung-kuo yen cheng yen chiu (2015): 74-82
Characterization of chemical, radiochemical and optical properties of a dual-labeled MMP-9 targeting peptide.
Authors: Azhdarinia, Ali and Wilganowski, Nathaniel and Robinson, Holly and Ghosh, Pradip and Kwon, Sunkuk and Lazard, Zawaunyka W and Davis, Alan R and Olmsted-Davis, Elizabeth and Sevick-Muraca, Eva M
Journal: Bioorganic & medicinal chemistry (2011): 3769-76