iFluor® 830 succinimidyl ester
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1527.88 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 830 |
Excitation (nm) | 830 |
Emission (nm) | 867 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
iFluor® 830 maleimide |
iFluor® 830 acid |
Overview | ![]() ![]() |
See also: Amine Reactive Dyes and Probes for Conjugation, iFluor® Dyes and Kits, Wide Field (WF) Fluorescent Microscopy
Molecular weight 1527.88 | Absorbance (nm) 830 | Excitation (nm) 830 | Emission (nm) 867 |
In vivo fluorescence imaging uses a sensitive camera to detect the fluorescence emission from fluorophores in whole-body living small animals. Fluorophores with long emissions at the infrared (IR) region are generally preferred to overcome photon attenuation in living tissue. Recent advances in imaging strategies and reporter techniques for in vivo fluorescence imaging include novel approaches to improve the specificity and affinity of the probes and to modulate and amplify the signal at target sites for enhanced sensitivity. Further developments aim to achieve high-resolution, multimodality, and lifetime-based in vivo fluorescence imaging. Our iFluor® 830 is designed to label proteins and other biomolecules with infrared fluorescence. Conjugates prepared with iFluor® 830 have excitation and emission maxima in the IR range. iFluor® 830 dye emission is a unique color for spectrum flow cytometry as it is well separated from commonly used far-red fluorophores such as Cy5, Cy7, or allophycocyanin (APC), facilitating multicolor analysis. This fluorophore is also useful for small animal in vivo imaging applications or other imaging applications requiring IR detection. iFluor® 830 succinimidyl ester is amine-reactive and can be readily used to conjugate amine-containing biomolecules, particularly antibodies.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 830 succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 65.45 µL | 327.251 µL | 654.502 µL | 3.273 mL | 6.545 mL |
5 mM | 13.09 µL | 65.45 µL | 130.9 µL | 654.502 µL | 1.309 mL |
10 mM | 6.545 µL | 32.725 µL | 65.45 µL | 327.251 µL | 654.502 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Absorbance (nm) | 830 |
Excitation (nm) | 830 |
Emission (nm) | 867 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 350 succinimidyl ester | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
iFluor® 405 succinimidyl ester | 403 | 427 | 370001 | 0.911 | 0.48 | 0.77 |
iFluor® 488 succinimidyl ester | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 514 succinimidyl ester | 511 | 527 | 750001 | 0.831 | 0.265 | 0.116 |
iFluor® 532 succinimidyl ester | 537 | 560 | 900001 | 0.681 | 0.26 | 0.16 |
iFluor® 555 succinimidyl ester | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 594 succinimidyl ester | 588 | 604 | 1800001 | 0.531 | 0.05 | 0.04 |
iFluor® 633 succinimidyl ester | 640 | 654 | 2500001 | 0.291 | 0.062 | 0.044 |
iFluor® 647 succinimidyl ester | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
Show More (35) |
Images

Figure 1. iFluor® 830 is designed to label proteins and other biomolecules with infrared fluorescence. Conjugates prepared with iFluor® 830 have excitation and emission maxima in the IR range. iFluor® 830 succinimidyl ester is the most convenient reactive form for preparing the desired iFluor® 830 conjugates as it specifically and readily reacts with amino groups.
References
View all 15 references: Citation Explorer
Deep learning for in vivo near-infrared imaging.
Authors: Ma, Zhuoran and Wang, Feifei and Wang, Weizhi and Zhong, Yeteng and Dai, Hongjie
Journal: Proceedings of the National Academy of Sciences of the United States of America (2021)
Authors: Ma, Zhuoran and Wang, Feifei and Wang, Weizhi and Zhong, Yeteng and Dai, Hongjie
Journal: Proceedings of the National Academy of Sciences of the United States of America (2021)
Measuring Kidney Perfusion, pH, and Renal Clearance Consecutively Using MRI and Multispectral Optoacoustic Tomography.
Authors: Minhas, Atul S and Sharkey, Jack and Randtke, Edward A and Murray, Patricia and Wilm, Bettina and Pagel, Mark D and Poptani, Harish
Journal: Molecular imaging and biology (2020): 494-503
Authors: Minhas, Atul S and Sharkey, Jack and Randtke, Edward A and Murray, Patricia and Wilm, Bettina and Pagel, Mark D and Poptani, Harish
Journal: Molecular imaging and biology (2020): 494-503
Fast DNA Extraction with Polyacrylamide Microspheres for Polymerase Chain Reaction Detection.
Authors: Wang, Jun and Zhang, Shuyan and Xu, Xinhui and Xing, Yujun and Li, Zongru and Wang, Jinke
Journal: ACS omega (2020): 13829-13839
Authors: Wang, Jun and Zhang, Shuyan and Xu, Xinhui and Xing, Yujun and Li, Zongru and Wang, Jinke
Journal: ACS omega (2020): 13829-13839
Evaluation of Targeting Efficiency of Joints with Anticollagen II Antibodies.
Authors: Lofchy, Laren A and Vu, Vivian P and Banda, Nirmal K and Ramirez, Joseline Ramos and Smith, Weston J and Gifford, Geoffrey and Gaikwad, Hanmant and Scheinman, Robert I and Simberg, Dmitri
Journal: Molecular pharmaceutics (2019): 2445-2451
Authors: Lofchy, Laren A and Vu, Vivian P and Banda, Nirmal K and Ramirez, Joseline Ramos and Smith, Weston J and Gifford, Geoffrey and Gaikwad, Hanmant and Scheinman, Robert I and Simberg, Dmitri
Journal: Molecular pharmaceutics (2019): 2445-2451
A Modular Dual-Labeling Scaffold That Retains Agonistic Properties for Somatostatin Receptor Targeting.
Authors: Ghosh, Sukhen C and Rodriguez, Melissa and Carmon, Kendra S and Voss, Julie and Wilganowski, Nathaniel L and Schonbrunn, Agnes and Azhdarinia, Ali
Journal: Journal of nuclear medicine : official publication, Society of Nuclear Medicine (2017): 1858-1864
Authors: Ghosh, Sukhen C and Rodriguez, Melissa and Carmon, Kendra S and Voss, Julie and Wilganowski, Nathaniel L and Schonbrunn, Agnes and Azhdarinia, Ali
Journal: Journal of nuclear medicine : official publication, Society of Nuclear Medicine (2017): 1858-1864
Longitudinal monitoring of skin accumulation of nanocarriers and biologicals with fiber optic near infrared fluorescence spectroscopy (FONIRS).
Authors: Griffin, James I and Benchimol, Michael J and Simberg, Dmitri
Journal: Journal of controlled release : official journal of the Controlled Release Society (2017): 167-174
Authors: Griffin, James I and Benchimol, Michael J and Simberg, Dmitri
Journal: Journal of controlled release : official journal of the Controlled Release Society (2017): 167-174
Pharmacokinetic and Biodistribution Studies of HPMA Copolymer Conjugates in an Aseptic Implant Loosening Mouse Model.
Authors: Wei, Xin and Li, Fei and Zhao, Gang and Chhonker, Yashpal Singh and Averill, Christine and Galdamez, Josselyn and Purdue, P Edward and Wang, Xiaoyan and Fehringer, Edward V and Garvin, Kevin L and Goldring, Steven R and Alnouti, Yazen and Wang, Dong
Journal: Molecular pharmaceutics (2017): 1418-1428
Authors: Wei, Xin and Li, Fei and Zhao, Gang and Chhonker, Yashpal Singh and Averill, Christine and Galdamez, Josselyn and Purdue, P Edward and Wang, Xiaoyan and Fehringer, Edward V and Garvin, Kevin L and Goldring, Steven R and Alnouti, Yazen and Wang, Dong
Journal: Molecular pharmaceutics (2017): 1418-1428
Influence of chelator and near-infrared dye labeling on biocharacteristics of dual-labeled trastuzumab-based imaging agents.
Authors: Wang, Xuejuan and Aldrich, Melissa B and Yang, Zhi and Zhou, Nina and Xie, Qing and Liu, Chen and Sevick-Muraca, Eva
Journal: Chinese journal of cancer research = Chung-kuo yen cheng yen chiu (2016): 362-9
Authors: Wang, Xuejuan and Aldrich, Melissa B and Yang, Zhi and Zhou, Nina and Xie, Qing and Liu, Chen and Sevick-Muraca, Eva
Journal: Chinese journal of cancer research = Chung-kuo yen cheng yen chiu (2016): 362-9
Preclinical characterization and validation of a dual-labeled trastuzumab-based imaging agent for diagnosing breast cancer.
Authors: Wang, Xuejuan and Aldrich, Melissa B and Marshall, Milton V and Sevick-Muraca, Eva M
Journal: Chinese journal of cancer research = Chung-kuo yen cheng yen chiu (2015): 74-82
Authors: Wang, Xuejuan and Aldrich, Melissa B and Marshall, Milton V and Sevick-Muraca, Eva M
Journal: Chinese journal of cancer research = Chung-kuo yen cheng yen chiu (2015): 74-82
Characterization of chemical, radiochemical and optical properties of a dual-labeled MMP-9 targeting peptide.
Authors: Azhdarinia, Ali and Wilganowski, Nathaniel and Robinson, Holly and Ghosh, Pradip and Kwon, Sunkuk and Lazard, Zawaunyka W and Davis, Alan R and Olmsted-Davis, Elizabeth and Sevick-Muraca, Eva M
Journal: Bioorganic & medicinal chemistry (2011): 3769-76
Authors: Azhdarinia, Ali and Wilganowski, Nathaniel and Robinson, Holly and Ghosh, Pradip and Kwon, Sunkuk and Lazard, Zawaunyka W and Davis, Alan R and Olmsted-Davis, Elizabeth and Sevick-Muraca, Eva M
Journal: Bioorganic & medicinal chemistry (2011): 3769-76
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)