iFluor® 568 succinimidyl ester

iFluor® 568 Succinimidyl Ester is an orange-red fluorescent, amine-reactive dye for covalent labeling of antibodies and other proteins in fluorescence-based research applications.

Amine-Reactive Functionality: NHS ester selectively reacts with primary amines, including lysine residues.
Spectral Properties: Excitation at 568 nm and emission at 587 nm.
Spectral Compatibility: Alternative to Alexa Fluor® 568 for fluorescence detection.
Applications: Suitable for fluorescent conjugation of antibodies and other amine-containing proteins.

<p>HeLa cells were incubated with mouse anti-tubulin followed with iFluor<sup>TM</sup> 568 goat anti-mouse IgG conjugate (Red). Cell nuclei were stained with DAPI (Blue, Cat#17507).</p>

Protocol

Catalog	Size	Price
1049	1 mg	Price
71049	100 ug	Price
71522	5 mg	Price
71572	10 mg	Price

Overview

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 568 dyes have fluorescence excitation and emission maxima essentially identical to those of Alexa Fluor® 568 dyes. These spectral characteristics make them excellent alternatives to Alexa Fluor® 568 dyes (Alexa Fluor® is the trademark of Invitrogen). Under the same conditions we tested, iFluor® 568-labeled anti-IgG and streptavidin conjugates are brighter and more photostable than the corresponding Alexa Fluo® 568 conjugates. iFluor® 568 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 568 succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	85.218 µL	426.09 µL	852.181 µL	4.261 mL	8.522 mL
5 mM	17.044 µL	85.218 µL	170.436 µL	852.181 µL	1.704 mL
10 mM	8.522 µL	42.609 µL	85.218 µL	426.09 µL	852.181 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1173.46 g/mol	=	mL	x	mM	=	mmol

Citations

View all 4 citations: Citation Explorer

SREBP2 restricts osteoclast differentiation and activity by regulating IRF7 and limits inflammatory bone erosion

Authors:

Kim, Haemin and Choi, In Ah and Umemoto, Akio and Bae, Seyeon and Kaneko, Kaichi and Mizuno, Masataka and Giannopoulou, Eugenia and Pannellini, Tania and Deng, Liang and Park-Min, Kyung-Hyun

Journal:

Bone Research (2024): 48

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification

Authors:

Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined

Journal:

Metabolomics (Los Angel) (2017): 2153--0769

Suramin inhibits cullin-RING E3 ubiquitin ligases

Authors:

Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined

Journal:

Proceedings of the National Academy of Sciences (2016): E2011--E2018

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function

Authors:

Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined

Journal:

International Journal of Cancer (2012): E425--E436

References

View all 49 references: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes

Authors:

Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.

Journal:

Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling

Authors:

Zhang S, Tan HC, Ooi EE.

Journal:

J Vis Exp. (2011)

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells

Authors:

Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.

Journal:

Biochem Biophys Res Commun (2011): 211

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Authors:

Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.

Journal:

Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers

Authors:

Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.

Journal:

J Microbiol Methods (2011): 119

Physical properties

Molecular weight	1173.46
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.34
Correction factor (280 nm)	0.15
Extinction coefficient (cm ^-1 M ^-1)	100000 ¹
Excitation (nm)	568
Emission (nm)	587
Quantum yield	0.57 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 28, 2026