iFluor® 665 succinimidyl ester

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 532, 555, 633, and 647 nm). The iFluor® 665 family has spectral properties similar to those of Alexa Fluor® 660 (Alexa Fluor® is the trademark of Invitrogen). In addition, the fluorescence of iFluor® 665 is pH-insensitive over a broad range, pH 3-11. These spectral characteristics make this new dye family an excellent alternative to Alexa Fluor® 660. Under the same conditions, iFluor® 665 gives a stronger fluorescence signal on some antibodies we tested. iFluor® 665 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups.

Protocol

COA

Catalog	Size	Price
1550	100 ug	Price
1551	1 mg	Price
1552	5 mg	Price
1553	10 mg	Price

References

View all 7 references: Citation Explorer

Recognition of Invasive Prostate Cancer Using a GHRL Polypeptide Probe Targeting GHSR in a Mouse Model In Vivo.

Authors:

Ye, Huamao and Yang, Yue and Chen, Rui and Shi, Xiaolei and Fang, Yu and Yang, Jun and Dong, Yuanzhen and Chen, Lili and Xia, Jianghua and Wang, Chao and Yang, Chenghua and Feng, Jun and Wang, Yang and Feng, Xiang and Lü, Chen

Journal:

Current pharmaceutical design (2020): 1614-1621

A rapid sensitive, flow cytometry-based method for the detection of Plasmodium vivax-infected blood cells.

Authors:

Roobsoong, Wanlapa and Maher, Steven P and Rachaphaew, Nattawan and Barnes, Samantha J and Williamson, Kim C and Sattabongkot, Jetsumon and Adams, John H

Journal:

Malaria journal (2014): 55

Reengineering the optical absorption cross-section of photosynthetic reaction centers.

Authors:

Dutta, Palash K and Lin, Su and Loskutov, Andrey and Levenberg, Symon and Jun, Daniel and Saer, Rafael and Beatty, J Thomas and Liu, Yan and Yan, Hao and Woodbury, Neal W

Journal:

Journal of the American Chemical Society (2014): 4599-604

Comparison of a chimeric anti-carcinoembryonic antigen antibody conjugated with visible or near-infrared fluorescent dyes for imaging pancreatic cancer in orthotopic nude mouse models.

Authors:

Maawy, Ali A and Hiroshima, Yukihiko and Kaushal, Sharmeela and Luiken, George A and Hoffman, Robert M and Bouvet, Michael

Journal:

Journal of biomedical optics (2013): 126016

Nucleic acid sandwich hybridization assay with quantum dot-induced fluorescence resonance energy transfer for pathogen detection.

Authors:

Chou, Cheng-Chung and Huang, Yi-Han

Journal:

Sensors (Basel, Switzerland) (2012): 16660-72

Physical properties

Molecular weight	1210.53
Solvent	DMSO

Spectral properties

Absorbance (nm)	661
Correction factor (260 nm)	0.12
Correction factor (280 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	110,000 ¹
Excitation (nm)	667
Emission (nm)	692
Quantum yield	0.22 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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