iFluor® 690 succinimidyl ester

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, 633, and 647 nm) or other excitation sources. iFluor® 690 dyes have fluorescence excitation and emission maxima of ~685 nm and ~704 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 700 labeling dye (Alexa Fluor® is the trademark of Invitrogen). iFluor® 690 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. Our preliminary tests indicated that it has superior FRET when conjugated to PE, making it an excellent acceptor dye for preparing tandems with APC, PE, and PerCP. iFluor® 690-based tandems provide unique colors that are extremely useful for spectral flow cytometry applications.

Flow cytometry analysis of PBMC stained with iFluor® 690 anti-human CD3 *SK7* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the iFluor® 690 specific R4-A channel.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
1042	1 mg	Price

References

View all 8 references: Citation Explorer

Multiplexed non-invasive tumor imaging of glucose metabolism and receptor-ligand engagement using dark quencher FRET acceptor.

Authors:

Rudkouskaya, Alena and Sinsuebphon, Nattawut and Ochoa, Marien and Chen, Sez-Jade and Mazurkiewicz, Joseph E and Intes, Xavier and Barroso, Margarida

Journal:

Theranostics (2020): 10309-10325

Performance of optoacoustic and fluorescence imaging in detecting deep-seated fluorescent agents.

Authors:

Chen, Zhenyue and Deán-Ben, Xosé Luís and Gottschalk, Sven and Razansky, Daniel

Journal:

Biomedical optics express (2018): 2229-2239

An enzymatically-sensitized sequential and concentric energy transfer relay self-assembled around semiconductor quantum dots.

Authors:

Samanta, Anirban and Walper, Scott A and Susumu, Kimihiro and Dwyer, Chris L and Medintz, Igor L

Journal:

Nanoscale (2015): 7603-14

Multicolor detection of rare tumor cells in blood using a novel flow cytometry-based system.

Authors:

Watanabe, Masaru and Uehara, Yuri and Yamashita, Namiko and Fujimura, Yuu and Nishio, Kaori and Sawada, Takeshi and Takeda, Kazuo and Koizumi, Fumiaki and Koh, Yasuhiro

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2014): 206-13

Noninvasive and quantitative assessment of in vivo fetomaternal interface angiogenesis using RGD-based fluorescence.

Authors:

Keramidas, M and Lavaud, J and Sergent, F and Hoffmann, P and Brouillet, S and Feige, J-J and Coll, J-L and Alfaidy, N

Journal:

BioMed research international (2014): 309082

Physical properties

Molecular weight	1462.75
Solvent	DMSO

Spectral properties

Absorbance (nm)	682
Correction factor (260 nm)	0.09
Correction factor (280 nm)	0.06
Extinction coefficient (cm ^-1 M ^-1)	220000 ¹
Excitation (nm)	685
Emission (nm)	704
Quantum yield	0.30 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on September 28, 2025