Name: iFluor® 647 succinimidyl ester
Brand: AAT Bioquest
SKU: 1031
Availability: InStock

iFluor® 647 succinimidyl ester

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). The iFluor® 647 family has spectral properties essentially identical to those of Cy5®. Compared to Cy5® probes, iFluor® 647 reagents have much stronger fluorescence and higher photostability. Their fluorescence is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family an excellent alternative to Cy5® and Alexa Fluor® 647 (Cy5® and Alexa Fluor® are the trademarks of GE Health Care and Invitrogen). iFluor® 647 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups.

<p>HeLa cells were stained with mouse anti-tubulin followed with iFluor<sup>TM</sup> 647 goat anti-mouse IgG (H+L) (red); and nuclei were stained with DAPI (blue).</p>

Protocol

COO

COA

Catalog	Size	Price
1031	1 mg	Price
71031	100 ug	Price
71510	5 mg	Price
71560	10 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 647 succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	78.452 µL	392.261 µL	784.523 µL	3.923 mL	7.845 mL
5 mM	15.69 µL	78.452 µL	156.905 µL	784.523 µL	1.569 mL
10 mM	7.845 µL	39.226 µL	78.452 µL	392.261 µL	784.523 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1274.66 g/mol	=	mL	x	mM	=	mmol

Citations

View all 67 citations: Citation Explorer

Molecular exaptation by the integrin $\alpha$I domain

Authors:

Hollis, Jeremy A and Chan, Matthew C and Malik, Harmit S and Campbell, Melody G

Journal:

Science Advances (2025): eadx9567

Human Placental Extract Enhances Neonatal Intestinal Epithelial Reparative Capacity in Porcine Models

Authors:

McKinney-Aguirre, Caroline A and Stewart, Amy S and Freund, John M and Singh, Abhishek and Hojnacki, Jenna I and Tomblyn, Seth and Berger, Michael B and Washburn, Scott A and Gonzalez, Liara M

Journal:

Cellular and Molecular Gastroenterology and Hepatology (2025): 101619

Targeting Hippo Signaling Pathway with a Boron Derivative, Sodium Pentaborate Pentahydrate (NaB): Therapeutic Strategies in Colorectal Cancer

Authors:

Y{\"u}ksel, B{\"u}{\c{s}}ra and {\c{S}}ahin, Fikrettin and T{\"u}rkel, Nezaket

Journal:

Pharmaceuticals (2025): 1171

IRF7 in peripheral monocytes drives BBB disruption in anti-NMDAR encephalitis

Authors:

Li, Qihui and Xiao, Li and Li, Jianfang and Huang, Yu and Shen, Shishi and Li, Huilu and Peng, Fuhua and He, Huichang and Huang, Shen and Sun, Jian and others,

Journal:

Brain, Behavior, and Immunity (2025)

Pentoxifylline Enhances the Effects of Doxorubicin and Bleomycin on Apoptosis, Caspase Activity, and Cell Cycle While Reducing Proliferation and Senescence in Hodgkin’s Disease Cell Line

Authors:

Guti{\'e}rrez-Ortiz, Jes{\'u}s A and Gonzalez-Ramella, Oscar and Solorzano-Ibarra, Fabiola and Bravo-Cuellar, Alejandro and Hern{\'a}ndez-Flores, Georgina and Padilla-Ortega, Jos{\'e} A and Pelayo-Rubio, Fernanda and Vazquez-Urrutia, Jorge R and Ortiz-Lazareno, Pablo C

Journal:

Current Issues in Molecular Biology (2025): 593

References

View all 49 references: Citation Explorer

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Authors:

Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.

Journal:

Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers

Authors:

Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.

Journal:

J Microbiol Methods (2011): 119

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells

Authors:

Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.

Journal:

Biochem Biophys Res Commun (2011): 211

Visualizing dengue virus through Alexa Fluor labeling

Authors:

Zhang S, Tan HC, Ooi EE.

Journal:

J Vis Exp. (2011)

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes

Authors:

Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.

Journal:

Mol Biosyst (2011): 1766

Physical properties

Molecular weight	1274.66
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.03
Correction factor (280 nm)	0.03
Correction factor (656 nm)	0.0793
Extinction coefficient (cm ^-1 M ^-1)	250000 ¹
Excitation (nm)	656
Emission (nm)	670
Quantum yield	0.25 ¹

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on September 20, 2025