iFluor® 500 succinimidyl ester is an amine-reactive fluorescent dye designed for covalent labeling of proteins, antibodies, and other biomolecules containing accessible primary amine groups. The succinimidyl ester group reacts with lysine residues and N-terminal amines under appropriate buffer conditions, enabling preparation of fluorescent bioconjugates for detection and imaging applications.

This dye has excitation and emission maxima of approximately 500/520 nm, placing it between commonly used Alexa Fluor® 488 and Alexa Fluor® 514 labeling dyes (Alexa Fluor® is the trademark of ThermoFisher Scentific). iFluor® 500 succinimidyl ester is suitable for protein and antibody labeling workflows where a green fluorescent dye with intermediate spectral properties is desired for flow cytometry, fluorescence microscopy, or other fluorescence-based assays.