iFluor® 647 azide

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 647 dyes have fluorescence excitation and emission maxima of ~656 nm and ~670 nm respectively. iFluor® 647 family has spectral properties essentially identical to those of Cy5® (Cy5® is the trademark of GE Healthcare). Compared to Cy5® probes, iFluor® 647 reagents have much stronger fluorescence and higher photostability. Their fluorescence is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family an excellent alternative to Cy5® and Alexa Fluor® 647 (Cy5® and Alexa Fluor® are the trademarks of Invitrogen and GE Health Care). iFluor® 647 azide is reasonably stable and shows good reactivity and selectivity with the alkyne group.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 647 azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	96.051 µL	480.257 µL	960.513 µL	4.803 mL	9.605 mL
5 mM	19.21 µL	96.051 µL	192.103 µL	960.513 µL	1.921 mL
10 mM	9.605 µL	48.026 µL	96.051 µL	480.257 µL	960.513 µL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 405 azide	403	427	37000¹	0.91¹	0.48	0.77
iFluor® 488 azide	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 555 azide	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 594 azide	587	603	200000¹	0.53¹	0.05	0.04
iFluor® 647 Styramide Superior Replacement for Alexa Fluor 647 tyramide	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 647 TCO	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 647 Tetrazine	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 790 Azide	787	812	250000¹	0.13¹	0.1	0.09

Citations

View all 31 citations: Citation Explorer

CST Is Epistatic With Shieldin to Limit DNA Double-Strand Break End Resection and Promote Repair During Igh Class Switch Recombination
Authors: Lescale, Chlo{\'e} and Marton, Timea and Vaysse, Amaury and Rode, Guillaume and Vincendeau, Estelle and Libri, Alice and Dossin, Fran{\c{c}}ois and Deriano, Ludovic
Journal: European Journal of Immunology (2025): e202451585

CDH17-targeting CAR-NK cells synergize with CD47 blockade for potent suppression of gastrointestinal cancers
Authors: Zheng, Liuhai and Ding, Youbing and Xu, Xiaolong and Wang, Huifang and Shi, Guangwei and Li, Yang and He, Yuanqiao and Gong, Yue and Zhang, Xiaodong and Wei, Jinxi and others,
Journal: Acta Pharmaceutica Sinica B (2025)

A multistage drug delivery approach for colorectal primary tumors and lymph node metastases
Authors: Yuan, Yihang and Lin, Quanjun and Feng, Hai-Yi and Zhang, Yunpeng and Lai, Xing and Zhu, Mao-Hua and Wang, Jue and Shi, Jiangpei and Huang, Yanhu and Zhang, Lele and others,
Journal: Nature Communications (2025): 1439

Highly potent novel multi-armoured IL13R$\alpha$2 CAR-T subverts the immunosuppressive microenvironment of Glioblastoma
Authors: Mangolini, Maurizio and Srivastava, Saket and Souster, Emily and Yang, Yajing and Wang, Huimin and Karattil, Rajesh and Schultz, Liam and Ma, Biao and Pombal, Diana and Greenig, Matthew and others,
Journal: bioRxiv (2025): 2025--01

Circ\_001653 alleviates sepsis associated-acute kidney injury by recruiting BUD13 to regulate KEAP1/NRF2/HO-1 signaling pathway
Authors: Li, Xinxin and Zhou, Wei and Chen, Jianjun and Zhou, Liangliang and Li, Yingbing and Wu, Xufeng and Peng, Xia
Journal: Journal of Inflammation (2024): 1--13

References

View all 49 references: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes
Authors: Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.
Journal: Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells
Authors: Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.
Journal: Biochem Biophys Res Commun (2011): 211

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
Authors: Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.
Journal: Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers
Authors: Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.
Journal: J Microbiol Methods (2011): 119

Page updated on May 19, 2025

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Catalog Number	1091
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Physical properties

Molecular weight	1041.11
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.03
Correction Factor (280 nm)	0.03
Correction Factor (656 nm)	0.0793
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	656
Emission (nm)	670
Quantum yield	0.25¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501