iFluor® 647 azide

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 647 dyes have fluorescence excitation and emission maxima of ~656 nm and ~670 nm respectively. iFluor® 647 family has spectral properties essentially identical to those of Cy5® (Cy5® is the trademark of GE Healthcare). Compared to Cy5® probes, iFluor® 647 reagents have much stronger fluorescence and higher photostability. Their fluorescence is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family an excellent alternative to Cy5® and Alexa Fluor® 647 (Cy5® and Alexa Fluor® are the trademarks of Invitrogen and GE Health Care). iFluor® 647 azide is reasonably stable and shows good reactivity and selectivity with the alkyne group.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 647 azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	96.051 µL	480.257 µL	960.513 µL	4.803 mL	9.605 mL
5 mM	19.21 µL	96.051 µL	192.103 µL	960.513 µL	1.921 mL
10 mM	9.605 µL	48.026 µL	96.051 µL	480.257 µL	960.513 µL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)	Correction Factor (656 nm)
iFluor® 488 azide	491	516	75000¹	0.9¹	0.21	0.11	-
iFluor® 555 azide	557	570	100000¹	0.64¹	0.23	0.14	-
iFluor® 647 Styramide Superior Replacement for Alexa Fluor 647 tyramide	656	670	250000¹	0.25¹	0.03	0.03	0.0793
iFluor® 647 Tyramide	656	670	250000¹	0.25¹	0.03	0.03	0.0793
iFluor® 647 TCO	656	670	250000¹	0.25¹	0.03	0.03	0.0793
iFluor® 647 Tetrazine	656	670	250000¹	0.25¹	0.03	0.03	0.0793
iFluor® 405 azide	403	427	37000¹	0.91¹	0.48	0.77	-
iFluor® 790 Azide	787	812	250000¹	0.13¹	0.1	0.09	-

Citations

View all 27 citations: Citation Explorer

Circ\_001653 alleviates sepsis associated-acute kidney injury by recruiting BUD13 to regulate KEAP1/NRF2/HO-1 signaling pathway
Authors: Li, Xinxin and Zhou, Wei and Chen, Jianjun and Zhou, Liangliang and Li, Yingbing and Wu, Xufeng and Peng, Xia
Journal: Journal of Inflammation (2024): 1--13

Novel immunogenic cell death inducer combined with autophagy inhibitor to amplify photodynamic synergistic immunotherapy for triple-negative breast cancer
Authors: Yuan, Gankun and Yang, Ruyue and Wen, Wenjing and Wei, Zhaoyi and Song, Meiru and Zhang, Lingyang and Hou, Kun and Liang, Gaofeng
Journal: (2024)

The tandem CD33-CLL1 CAR-T as an approach to treat acute myeloid leukemia: The tandem CLL1/CD33 CAR-T to treat AML
Authors: Wang, Huiru and Feng, Shanglong and Zhu, Yanliang and Zhang, Yafeng and Zhou, Ziwei and Nian, Zhigang and Lu, Xueqin and Peng, Peng and Wu, Shu and Zhou, Li
Journal: Blood Transfusion (2024)

Cryo-shocked tumor cells deliver CRISPR-Cas9 for lung cancer regression by synthetic lethality
Authors: Liu, Feng and Xin, Minhang and Feng, Huiheng and Zhang, Wentao and Liao, Ziyan and Sheng, Tao and Wen, Ping and Wu, Qing and Liang, Tingxizi and Shi, Jiaqi and others,
Journal: Science Advances (2024): eadk8264

Comparing the Malignant Properties of Parental and a knock-in version of HCT116 cell line expressing the CDK2-mutant of eukaryotic Elongation Factor 2 (eEF2)
Authors: Y{\"u}ksel, B{\"u}{\c{s}}ra and T{\"u}rkel, Nezaket and {\c{S}}ahin, Fikrettin and DENIZ, ASLI AYSEN HIZLI
Journal: (2024)

References

View all 49 references: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes
Authors: Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.
Journal: Mol Biosyst (2011): 1766

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers
Authors: Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.
Journal: J Microbiol Methods (2011): 119

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
Authors: Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.
Journal: Zhen Ci Yan Jiu (2011): 262

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells
Authors: Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.
Journal: Biochem Biophys Res Commun (2011): 211

Visualizing dengue virus through Alexa Fluor labeling
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)

Page updated on October 22, 2024

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Catalog Number	1091
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Physical properties

Molecular weight	1041.11
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.03
Correction Factor (280 nm)	0.03
Correction Factor (656 nm)	0.0793
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	656
Emission (nm)	670
Quantum yield	0.25¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501