iFluor® 647 azide
Ordering information
Price | |
Catalog Number | |
Unit Size | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1041.11 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.03 |
Correction Factor (280 nm) | 0.03 |
Correction Factor (656 nm) | 0.0793 |
Extinction coefficient (cm -1 M -1) | 2500001 |
Excitation (nm) | 656 |
Emission (nm) | 670 |
Quantum yield | 0.251 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
See also: Antibodies and Proteomics, Antibody and Protein Labeling, Bioconjugation, iFluor® Dyes and Kits
Molecular weight 1041.11 | Correction Factor (260 nm) 0.03 | Correction Factor (280 nm) 0.03 | Correction Factor (656 nm) 0.0793 | Extinction coefficient (cm -1 M -1) 2500001 | Excitation (nm) 656 | Emission (nm) 670 | Quantum yield 0.251 |
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 647 dyes have fluorescence excitation and emission maxima of ~656 nm and ~670 nm respectively. iFluor® 647 family has spectral properties essentially identical to those of Cy5® (Cy5® is the trademark of GE Healthcare). Compared to Cy5® probes, iFluor® 647 reagents have much stronger fluorescence and higher photostability. Their fluorescence is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family an excellent alternative to Cy5® and Alexa Fluor® 647 (Cy5® and Alexa Fluor® are the trademarks of Invitrogen and GE Health Care). iFluor® 647 azide is reasonably stable and shows good reactivity and selectivity with the alkyne group.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 647 azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 96.051 µL | 480.257 µL | 960.513 µL | 4.803 mL | 9.605 mL |
5 mM | 19.21 µL | 96.051 µL | 192.103 µL | 960.513 µL | 1.921 mL |
10 mM | 9.605 µL | 48.026 µL | 96.051 µL | 480.257 µL | 960.513 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Correction Factor (260 nm) | 0.03 |
Correction Factor (280 nm) | 0.03 |
Correction Factor (656 nm) | 0.0793 |
Extinction coefficient (cm -1 M -1) | 2500001 |
Excitation (nm) | 656 |
Emission (nm) | 670 |
Quantum yield | 0.251 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) | Correction Factor (656 nm) |
iFluor® 488 azide | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 | - |
iFluor® 555 azide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 | - |
iFluor® 647 Styramide *Superior Replacement for Alexa Fluor 647 tyramide* | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 | 0.0793 |
iFluor® 647 Tyramide | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 | 0.0793 |
iFluor® 647 TCO | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 | 0.0793 |
iFluor® 647 Tetrazine | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 | 0.0793 |
iFluor™ 405 azide | 403 | 427 | 370001 | 0.911 | 0.48 | 0.77 | - |
Images

Figure 1. Click chemistry is a method for attaching a probe or substrate of interest to a specific biomolecule, a process called bioconjugation. The possibility of attaching fluorophores and other reporter molecules has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an azide with an alkyne to form a 5-membered heteroatom ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC).
Citations
View all 17 citations: Citation Explorer
Inhibition of MYC suppresses programmed cell death ligand-1 expression and enhances immunotherapy in triple-negative breast cancer
Authors: Li, Xintong and Tang, Lin and Chen, Qin and Cheng, Xumin and Liu, Yiqiu and Wang, Cenzhu and Zhu, Chengjun and Xu, Kun and Gao, Fangyan and Huang, Jinyi and others,
Journal: Chinese Medical Journal (2022): 10--1097
Authors: Li, Xintong and Tang, Lin and Chen, Qin and Cheng, Xumin and Liu, Yiqiu and Wang, Cenzhu and Zhu, Chengjun and Xu, Kun and Gao, Fangyan and Huang, Jinyi and others,
Journal: Chinese Medical Journal (2022): 10--1097
Immune-regulating bimetallic metal-organic framework nanoparticles designed for cancer immunotherapy
Authors: Dai, Zan and Wang, Qiaoyun and Tang, Jie and Wu, Min and Li, Haoze and Yang, Yannan and Zhen, Xu and Yu, Chengzhong
Journal: Biomaterials (2022): 121261
Authors: Dai, Zan and Wang, Qiaoyun and Tang, Jie and Wu, Min and Li, Haoze and Yang, Yannan and Zhen, Xu and Yu, Chengzhong
Journal: Biomaterials (2022): 121261
Site-specific labeling and functional efficiencies of human fibroblast growth Factor-1 with a range of fluorescent Dyes in the flexible N-Terminal region and a rigid $\beta$-turn region
Authors: Mohale, Mamello and Gundampati, Ravi Kumar and Kumar, Thallapuranam Krishnaswamy Suresh and Heyes, Colin D
Journal: Analytical biochemistry (2022): 114524
Authors: Mohale, Mamello and Gundampati, Ravi Kumar and Kumar, Thallapuranam Krishnaswamy Suresh and Heyes, Colin D
Journal: Analytical biochemistry (2022): 114524
Directed cell migration towards softer environments
Authors: Isomursu, Aleksi and Park, Keun-Young and Hou, Jay and Cheng, Bo and Mathieu, Mathilde and Shamsan, Ghaidan A and Fuller, Benjamin and Kasim, Jesse and Mahmoodi, M Mohsen and Lu, Tian Jian and others,
Journal: Nature Materials (2022): 1081--1090
Authors: Isomursu, Aleksi and Park, Keun-Young and Hou, Jay and Cheng, Bo and Mathieu, Mathilde and Shamsan, Ghaidan A and Fuller, Benjamin and Kasim, Jesse and Mahmoodi, M Mohsen and Lu, Tian Jian and others,
Journal: Nature Materials (2022): 1081--1090
Poly ADP-ribosylation of SET8 leads to aberrant H4K20 methylation in mammalian nuclear genome
Authors: Est{\`e}ve, Pierre-Olivier and Sen, Sagnik and Vishnu, Udayakumar S and Ruse, Cristian and Chin, Hang Gyeong and Pradhan, Sriharsa
Journal: Communications biology (2022): 1--18
Authors: Est{\`e}ve, Pierre-Olivier and Sen, Sagnik and Vishnu, Udayakumar S and Ruse, Cristian and Chin, Hang Gyeong and Pradhan, Sriharsa
Journal: Communications biology (2022): 1--18
The PARP1 Inhibitor Niraparib Represses DNA Damage Repair and Synergizes with Temozolomide for Antimyeloma Effects
Authors: Shen, Hong-Yuan and Tang, Hai-Long and Zheng, Yan-Hua and Feng, Juan and Dong, Bao-Xia and Chen, Xie-Qun
Journal: Journal of Oncology (2022)
Authors: Shen, Hong-Yuan and Tang, Hai-Long and Zheng, Yan-Hua and Feng, Juan and Dong, Bao-Xia and Chen, Xie-Qun
Journal: Journal of Oncology (2022)
A Substance P (SP)/Neurokinin-1 Receptor Axis Promotes Perineural Invasion of Pancreatic Cancer and Is Affected by lncRNA LOC389641
Authors: Ji, Tengfei and Ma, Keqiang and Wu, Hongsheng and Cao, Tiansheng
Journal: Journal of Immunology Research (2022)
Authors: Ji, Tengfei and Ma, Keqiang and Wu, Hongsheng and Cao, Tiansheng
Journal: Journal of Immunology Research (2022)
Digital Light Processing 3D Printing of Gyroid Scaffold with Isosorbide-Based Photopolymer for Bone Tissue Engineering
Authors: Verisqa, Fiona and Cha, Jae-Ryung and Nguyen, Linh and Kim, Hae-Won and Knowles, Jonathan C
Journal: Biomolecules (2022): 1692
Authors: Verisqa, Fiona and Cha, Jae-Ryung and Nguyen, Linh and Kim, Hae-Won and Knowles, Jonathan C
Journal: Biomolecules (2022): 1692
SP/NK-1R Axis Promotes Perineural Invasion of Pancreatic Cancer and is Affected by lncRNA LOC389641
Authors: Ji, Tengfei and Ma, Keqiang and Wu, Hongsheng and Cao, Tiansheng
Journal: (2021)
Authors: Ji, Tengfei and Ma, Keqiang and Wu, Hongsheng and Cao, Tiansheng
Journal: (2021)
Efferocytosis induces macrophage proliferation to help resolve tissue injury
Authors: Gerlach, Brennan D and Ampomah, Patrick B and Yurdagul Jr, Arif and Liu, Chuang and Lauring, Max C and Wang, Xiaobo and Kasikara, Canan and Kong, Na and Shi, Jinjun and Tao, Wei and others,
Journal: Cell metabolism (2021): 2445--2463
Authors: Gerlach, Brennan D and Ampomah, Patrick B and Yurdagul Jr, Arif and Liu, Chuang and Lauring, Max C and Wang, Xiaobo and Kasikara, Canan and Kong, Na and Shi, Jinjun and Tao, Wei and others,
Journal: Cell metabolism (2021): 2445--2463
References
View all 49 references: Citation Explorer
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Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
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