iFluor® 647 Tetrazine
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| Quotation | Request |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1156.25 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (260 nm) | 0.03 |
| Correction Factor (280 nm) | 0.03 |
| Correction Factor (656 nm) | 0.0793 |
| Extinction coefficient (cm -1 M -1) | 2500001 |
| Excitation (nm) | 656 |
| Emission (nm) | 670 |
| Quantum yield | 0.251 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
See also: Antibodies and Proteomics, Antibody and Protein Labeling, Bioconjugation, iFluor® Dyes and Kits
Molecular weight 1156.25 | Correction Factor (260 nm) 0.03 | Correction Factor (280 nm) 0.03 | Correction Factor (656 nm) 0.0793 | Extinction coefficient (cm -1 M -1) 2500001 | Excitation (nm) 656 | Emission (nm) 670 | Quantum yield 0.251 |
The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction. iFluor® 647 tetrazine can be readily used to label TCO-modified biological molecules for fluorescence imaging and other fluorescence-based biological applications. The conjugates prepared with iFluor® 647 dye have spectral properties almost identical to the popular Cy5 and Alexa Fluor® 647. In most cases, antibody conjugates prepared with iFluor® 647 tend to have a higher signal/background ratio than the spectrally similar dye conjugates, such as Cy5 and Alexa Fluor® 647 (Alexa Fluor® is the trademark of Invitrogen).
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 647 Tetrazine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 86.486 µL | 432.432 µL | 864.865 µL | 4.324 mL | 8.649 mL |
| 5 mM | 17.297 µL | 86.486 µL | 172.973 µL | 864.865 µL | 1.73 mL |
| 10 mM | 8.649 µL | 43.243 µL | 86.486 µL | 432.432 µL | 864.865 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.03 |
| Correction Factor (280 nm) | 0.03 |
| Correction Factor (656 nm) | 0.0793 |
| Extinction coefficient (cm -1 M -1) | 2500001 |
| Excitation (nm) | 656 |
| Emission (nm) | 670 |
| Quantum yield | 0.251 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) | Correction Factor (656 nm) |
| iFluor® 647 maleimide | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 | 0.0793 |
| iFluor® 647 amine | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 | 0.0793 |
| iFluor® 647 hydrazide | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 | 0.0793 |
| iFluor® 647 alkyne | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 | 0.0793 |
| iFluor® 647 azide | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 | 0.0793 |
| iFluor® 647 Styramide *Superior Replacement for Alexa Fluor 647 tyramide* | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 | 0.0793 |
| iFluor® 647 Tyramide | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 | 0.0793 |
| iFluor® 647 TCO | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 | 0.0793 |
| iFluor® 488 Tetrazine | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 | - |
Show More (2) | |||||||
Images
Figure 1. The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. iFluor® 647 tetrazine can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis.
Citations
View all 8 citations: Citation Explorer
Immune-regulating bimetallic metal-organic framework nanoparticles designed for cancer immunotherapy
Authors: Dai, Zan and Wang, Qiaoyun and Tang, Jie and Wu, Min and Li, Haoze and Yang, Yannan and Zhen, Xu and Yu, Chengzhong
Journal: Biomaterials (2022): 121261
Authors: Dai, Zan and Wang, Qiaoyun and Tang, Jie and Wu, Min and Li, Haoze and Yang, Yannan and Zhen, Xu and Yu, Chengzhong
Journal: Biomaterials (2022): 121261
Site-specific labeling and functional efficiencies of human fibroblast growth Factor-1 with a range of fluorescent Dyes in the flexible N-Terminal region and a rigid $\beta$-turn region
Authors: Mohale, Mamello and Gundampati, Ravi Kumar and Kumar, Thallapuranam Krishnaswamy Suresh and Heyes, Colin D
Journal: Analytical biochemistry (2022): 114524
Authors: Mohale, Mamello and Gundampati, Ravi Kumar and Kumar, Thallapuranam Krishnaswamy Suresh and Heyes, Colin D
Journal: Analytical biochemistry (2022): 114524
Digital Light Processing 3D Printing of Gyroid Scaffold with Isosorbide-Based Photopolymer for Bone Tissue Engineering
Authors: Verisqa, Fiona and Cha, Jae-Ryung and Nguyen, Linh and Kim, Hae-Won and Knowles, Jonathan C
Journal: Biomolecules (2022): 1692
Authors: Verisqa, Fiona and Cha, Jae-Ryung and Nguyen, Linh and Kim, Hae-Won and Knowles, Jonathan C
Journal: Biomolecules (2022): 1692
SP/NK-1R Axis Promotes Perineural Invasion of Pancreatic Cancer and is Affected by lncRNA LOC389641
Authors: Ji, Tengfei and Ma, Keqiang and Wu, Hongsheng and Cao, Tiansheng
Journal: (2021)
Authors: Ji, Tengfei and Ma, Keqiang and Wu, Hongsheng and Cao, Tiansheng
Journal: (2021)
Efferocytosis induces macrophage proliferation to help resolve tissue injury
Authors: Gerlach, Brennan D and Ampomah, Patrick B and Yurdagul Jr, Arif and Liu, Chuang and Lauring, Max C and Wang, Xiaobo and Kasikara, Canan and Kong, Na and Shi, Jinjun and Tao, Wei and others,
Journal: Cell metabolism (2021): 2445--2463
Authors: Gerlach, Brennan D and Ampomah, Patrick B and Yurdagul Jr, Arif and Liu, Chuang and Lauring, Max C and Wang, Xiaobo and Kasikara, Canan and Kong, Na and Shi, Jinjun and Tao, Wei and others,
Journal: Cell metabolism (2021): 2445--2463
Enrichment of NPC1-deficient cells with the lipid LBPA stimulates autophagy, improves lysosomal function, and reduces cholesterol storage
Authors: Ilnytska, Olga and Lai, Kimberly and Gorshkov, Kirill and Schultz, Mark L and Tran, Bruce Nguyen and Jeziorek, Maciej and Kunkel, Thaddeus J and Azaria, Ruth D and McLoughlin, Hayley S and Waghalter, Miriam and others,
Journal: Journal of Biological Chemistry (2021)
Authors: Ilnytska, Olga and Lai, Kimberly and Gorshkov, Kirill and Schultz, Mark L and Tran, Bruce Nguyen and Jeziorek, Maciej and Kunkel, Thaddeus J and Azaria, Ruth D and McLoughlin, Hayley S and Waghalter, Miriam and others,
Journal: Journal of Biological Chemistry (2021)
Pharmacological targeting of Sam68 functions in colorectal cancer stem cells
Authors: Masibag, Angelique N and Bergin, Christopher J and Haebe, Joshua R and Zouggar, A{\"\i}cha and Shah, Muhammad S and Sandouka, Tamara and da Silva, Amanda Mendes and Desrochers, Fran{\c{c}}ois M and Fournier-Morin, Aube and Benoit, Yannick D
Journal: Iscience (2021): 103442
Authors: Masibag, Angelique N and Bergin, Christopher J and Haebe, Joshua R and Zouggar, A{\"\i}cha and Shah, Muhammad S and Sandouka, Tamara and da Silva, Amanda Mendes and Desrochers, Fran{\c{c}}ois M and Fournier-Morin, Aube and Benoit, Yannick D
Journal: Iscience (2021): 103442
Influence of particle geometry on gastrointestinal transit and absorption following oral administration
Authors: Li, Dong and Zhuang, Jie and He, Haisheng and Jiang, Sifan and Banerjee, Amrita and Lu, Yi and Wu, Wei and Mitragotri, Samir and Gan, Li and Qi, Jianping
Journal: ACS applied materials \& interfaces (2017): 42492--42502
Authors: Li, Dong and Zhuang, Jie and He, Haisheng and Jiang, Sifan and Banerjee, Amrita and Lu, Yi and Wu, Wei and Mitragotri, Samir and Gan, Li and Qi, Jianping
Journal: ACS applied materials \& interfaces (2017): 42492--42502
References
View all 4 references: Citation Explorer
A Self-Evaluating Photothermal Therapeutic Nanoparticle.
Authors: Wang, Yanfang and Du, Wei and Zhang, Tong and Zhu, Yu and Ni, Yanhan and Wang, Chenchen and Sierra Raya, Fatima Maria and Zou, Liwei and Wang, Longsheng and Liang, Gaolin
Journal: ACS nano (2020)
Authors: Wang, Yanfang and Du, Wei and Zhang, Tong and Zhu, Yu and Ni, Yanhan and Wang, Chenchen and Sierra Raya, Fatima Maria and Zou, Liwei and Wang, Longsheng and Liang, Gaolin
Journal: ACS nano (2020)
Modulation of Mitochondriotropic Properties of Cyanine Dyes by in Organello Copper-Free Click Reaction.
Authors: Negwer, Inka and Hirsch, Markus and Kaloyanova, Stefka and Brown, Tom and Peneva, Kalina and Butt, Hans-Jürgen and Koynov, Kaloian and Helm, Mark
Journal: Chembiochem : a European journal of chemical biology (2017): 1814-1818
Authors: Negwer, Inka and Hirsch, Markus and Kaloyanova, Stefka and Brown, Tom and Peneva, Kalina and Butt, Hans-Jürgen and Koynov, Kaloian and Helm, Mark
Journal: Chembiochem : a European journal of chemical biology (2017): 1814-1818
Size-matched alkyne-conjugated cyanine fluorophores to identify differences in protein glycosylation.
Authors: Burnham-Marusich, Amanda R and Plechaty, Anna M and Berninsone, Patricia M
Journal: Electrophoresis (2014): 2621-5
Authors: Burnham-Marusich, Amanda R and Plechaty, Anna M and Berninsone, Patricia M
Journal: Electrophoresis (2014): 2621-5
Application of a Double Aza-Michael Reaction in a 'Click, Click, Cy-Click' Strategy: From Bench to Flow.
Authors: Zang, Qin and Javed, Salim and Ullah, Farman and Zhou, Aihua and Knudtson, Christopher A and Bi, Danse and Basha, Fatima Z and Organ, Michael G and Hanson, Paul R
Journal: Synthesis (2011): 2743-2750
Authors: Zang, Qin and Javed, Salim and Ullah, Farman and Zhou, Aihua and Knudtson, Christopher A and Bi, Danse and Basha, Fatima Z and Organ, Michael G and Hanson, Paul R
Journal: Synthesis (2011): 2743-2750