iFluor® 647 Tetrazine

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	1272.44
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.03
Correction Factor (280 nm)	0.03
Correction Factor (656 nm)	0.0793
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	656
Emission (nm)	670
Quantum yield	0.25¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 647 Tetrazine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	78.589 µL	392.946 µL	785.892 µL	3.929 mL	7.859 mL
5 mM	15.718 µL	78.589 µL	157.178 µL	785.892 µL	1.572 mL
10 mM	7.859 µL	39.295 µL	78.589 µL	392.946 µL	785.892 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.03
Correction Factor (280 nm)	0.03
Correction Factor (656 nm)	0.0793
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	656
Emission (nm)	670
Quantum yield	0.25¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)	Correction Factor (656 nm)
iFluor® 647 maleimide	656	670	250000¹	0.25¹	0.03	0.03	0.0793
iFluor® 647 amine	656	670	250000¹	0.25¹	0.03	0.03	0.0793
iFluor® 647 hydrazide	656	670	250000¹	0.25¹	0.03	0.03	0.0793
iFluor® 647 alkyne	656	670	250000¹	0.25¹	0.03	0.03	0.0793
iFluor® 647 azide	656	670	250000¹	0.25¹	0.03	0.03	0.0793
iFluor® 647 Styramide Superior Replacement for Alexa Fluor 647 tyramide	656	670	250000¹	0.25¹	0.03	0.03	0.0793
iFluor® 647 Tyramide	656	670	250000¹	0.25¹	0.03	0.03	0.0793
iFluor® 647 TCO	656	670	250000¹	0.25¹	0.03	0.03	0.0793
iFluor® 488 Tetrazine	491	516	75000¹	0.9¹	0.21	0.11	-
iFluor® 555 Tetrazine	557	570	100000¹	0.64¹	0.23	0.14	-
iFluor® 594 Tetrazine	587	603	200000¹	0.53¹	0.05	0.04	-
Show More (2)

Images

Figure 1. The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. iFluor® 647 tetrazine can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis.

Citations

View all 8 citations: Citation Explorer

Immune-regulating bimetallic metal-organic framework nanoparticles designed for cancer immunotherapy
Authors: Dai, Zan and Wang, Qiaoyun and Tang, Jie and Wu, Min and Li, Haoze and Yang, Yannan and Zhen, Xu and Yu, Chengzhong
Journal: Biomaterials (2022): 121261

Site-specific labeling and functional efficiencies of human fibroblast growth Factor-1 with a range of fluorescent Dyes in the flexible N-Terminal region and a rigid $\beta$-turn region
Authors: Mohale, Mamello and Gundampati, Ravi Kumar and Kumar, Thallapuranam Krishnaswamy Suresh and Heyes, Colin D
Journal: Analytical biochemistry (2022): 114524

Digital Light Processing 3D Printing of Gyroid Scaffold with Isosorbide-Based Photopolymer for Bone Tissue Engineering
Authors: Verisqa, Fiona and Cha, Jae-Ryung and Nguyen, Linh and Kim, Hae-Won and Knowles, Jonathan C
Journal: Biomolecules (2022): 1692

SP/NK-1R Axis Promotes Perineural Invasion of Pancreatic Cancer and is Affected by lncRNA LOC389641
Authors: Ji, Tengfei and Ma, Keqiang and Wu, Hongsheng and Cao, Tiansheng
Journal: (2021)

Efferocytosis induces macrophage proliferation to help resolve tissue injury
Authors: Gerlach, Brennan D and Ampomah, Patrick B and Yurdagul Jr, Arif and Liu, Chuang and Lauring, Max C and Wang, Xiaobo and Kasikara, Canan and Kong, Na and Shi, Jinjun and Tao, Wei and others,
Journal: Cell metabolism (2021): 2445--2463

Enrichment of NPC1-deficient cells with the lipid LBPA stimulates autophagy, improves lysosomal function, and reduces cholesterol storage
Authors: Ilnytska, Olga and Lai, Kimberly and Gorshkov, Kirill and Schultz, Mark L and Tran, Bruce Nguyen and Jeziorek, Maciej and Kunkel, Thaddeus J and Azaria, Ruth D and McLoughlin, Hayley S and Waghalter, Miriam and others,
Journal: Journal of Biological Chemistry (2021)

Pharmacological targeting of Sam68 functions in colorectal cancer stem cells
Authors: Masibag, Angelique N and Bergin, Christopher J and Haebe, Joshua R and Zouggar, A{\"\i}cha and Shah, Muhammad S and Sandouka, Tamara and da Silva, Amanda Mendes and Desrochers, Fran{\c{c}}ois M and Fournier-Morin, Aube and Benoit, Yannick D
Journal: Iscience (2021): 103442

Influence of particle geometry on gastrointestinal transit and absorption following oral administration
Authors: Li, Dong and Zhuang, Jie and He, Haisheng and Jiang, Sifan and Banerjee, Amrita and Lu, Yi and Wu, Wei and Mitragotri, Samir and Gan, Li and Qi, Jianping
Journal: ACS applied materials \& interfaces (2017): 42492--42502

References

View all 4 references: Citation Explorer

A Self-Evaluating Photothermal Therapeutic Nanoparticle.
Authors: Wang, Yanfang and Du, Wei and Zhang, Tong and Zhu, Yu and Ni, Yanhan and Wang, Chenchen and Sierra Raya, Fatima Maria and Zou, Liwei and Wang, Longsheng and Liang, Gaolin
Journal: ACS nano (2020)

Modulation of Mitochondriotropic Properties of Cyanine Dyes by in Organello Copper-Free Click Reaction.
Authors: Negwer, Inka and Hirsch, Markus and Kaloyanova, Stefka and Brown, Tom and Peneva, Kalina and Butt, Hans-Jürgen and Koynov, Kaloian and Helm, Mark
Journal: Chembiochem : a European journal of chemical biology (2017): 1814-1818

Size-matched alkyne-conjugated cyanine fluorophores to identify differences in protein glycosylation.
Authors: Burnham-Marusich, Amanda R and Plechaty, Anna M and Berninsone, Patricia M
Journal: Electrophoresis (2014): 2621-5

Application of a Double Aza-Michael Reaction in a 'Click, Click, Cy-Click' Strategy: From Bench to Flow.
Authors: Zang, Qin and Javed, Salim and Ullah, Farman and Zhou, Aihua and Knudtson, Christopher A and Bi, Danse and Basha, Fatima Z and Organ, Michael G and Hanson, Paul R
Journal: Synthesis (2011): 2743-2750