iFluor® 594 Tetrazine

The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. iFluor® 594 tetrazine can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis.

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	1020.02
Solvent	DMSO

Spectral properties

Absorbance (nm)	587
Correction Factor (260 nm)	0.05
Correction Factor (280 nm)	0.04
Extinction coefficient (cm ^-1 M ^-1)	180000¹
Excitation (nm)	588
Emission (nm)	604
Quantum yield	0.53¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

1020.02

Absorbance (nm)

587

Correction Factor (260 nm)

0.05

Correction Factor (280 nm)

0.04

Extinction coefficient (cm ^-1 M ^-1)

180000¹

Excitation (nm)

588

Emission (nm)

604

Quantum yield

0.53¹

The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction. iFluor® 594 tetrazine can be readily used to label TCO-modified biological molecules for fluorescence imaging and other fluorescence-based biological applications. The conjugates prepared with iFluor® 594 dye have spectral properties almost identical to the popular Cy3.5 and Alexa Fluor® 594. In most cases, antibody conjugates prepared with iFluor® 594 tend to have lower background than the spectrally similar dye conjugates, such as Cy3.5 and Alexa Fluor® 594 (Alexa Fluor® is the trademark of Invitrogen).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 594 Tetrazine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	98.037 µL	490.186 µL	980.373 µL	4.902 mL	9.804 mL
5 mM	19.607 µL	98.037 µL	196.075 µL	980.373 µL	1.961 mL
10 mM	9.804 µL	49.019 µL	98.037 µL	490.186 µL	980.373 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Absorbance (nm)	587
Correction Factor (260 nm)	0.05
Correction Factor (280 nm)	0.04
Extinction coefficient (cm ^-1 M ^-1)	180000¹
Excitation (nm)	588
Emission (nm)	604
Quantum yield	0.53¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 594 maleimide	588	604	180000¹	0.53¹	0.05	0.04
iFluor® 594 Styramide Superior Replacement for Alexa Fluor 594 tyramide	588	604	180000¹	0.53¹	0.05	0.04
iFluor® 594 Tyramide	588	604	180000¹	0.53¹	0.05	0.04
iFluor® 594 TCO	588	604	180000¹	0.53¹	0.05	0.04
iFluor® 488 Tetrazine	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 555 Tetrazine	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 647 Tetrazine	656	670	250000¹	0.25¹	0.03	0.03

Images

Figure 1. The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. iFluor® 594 tetrazine can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis.

References

View all 50 references: Citation Explorer

Facile Click-Mediated Cell Imaging Strategy of Liposomal Azido Mannosamine Lipids via Metabolic or Nonmetabolic Glycoengineering.
Authors: Shen, Li and Cai, Kaimin and Yu, Jin and Cheng, Jianjun
Journal: ACS omega (2020): 14111-14115

Bioorthogonal Labeling Reveals Different Expression of Glycans in Mouse Hippocampal Neuron Cultures during Their Development.
Authors: Soares da Costa, Diana and Sousa, João C and Dá Mesquita, Sandro and Petkova-Yankova, Nevena I and Marques, Fernanda and Reis, Rui L and Sousa, Nuno and Pashkuleva, Iva
Journal: Molecules (Basel, Switzerland) (2020)

Surface-modified nanoerythrosomes for potential optical imaging diagnostics.
Authors: Fornasier, Marco and Porcheddu, Andrea and Casu, Anna and Raghavan, Srinivasa R and Jönsson, Peter and Schillén, Karin and Murgia, Sergio
Journal: Journal of colloid and interface science (2020): 246-253

Novel Liposomal Azido Mannosamine Lipids on Metabolic Cell Labeling and Imaging via Cu-Free Click Chemistry.
Authors: Shen, Li and Cai, Kaimin and Yu, Jin and Cheng, Jianjun
Journal: Bioconjugate chemistry (2019): 2317-2322

One-Pot Synthesis of Triazolobenzodiazepines Through Decarboxylative [3 + 2] Cycloaddition of Nonstabilized Azomethine Ylides and Cu-Free Click Reactions.
Authors: Ma, Xiaoming and Zhang, Xiaofeng and Qiu, Weiqi and Zhang, Wensheng and Wan, Bruce and Evans, Jason and Zhang, Wei
Journal: Molecules (Basel, Switzerland) (2019)

Correction to "Photochemical Formation of Dibenzosilacyclohept-4-yne for Cu-Free Click Chemistry with Azides and 1,2,4,5-Tetrazines".
Authors: Martínek, Marek and Filipová, Lenka and Galeta, Juraj and Ludvíková, Lucie and Klán, Petr
Journal: Organic letters (2019): 8506

Targeting Mutated Plus Germline Epitopes Confers Pre-clinical Efficacy of an Instantly Formulated Cancer Nano-Vaccine.
Authors: Mohsen, Mona O and Vogel, Monique and Riether, Carsten and Muller, Julius and Salatino, Silvia and Ternette, Nicola and Gomes, Ariane C and Cabral-Miranda, Gustavo and El-Turabi, Aadil and Ruedl, Christiane and Kundig, Thomas M and Dermime, Said and Knuth, Alexander and Speiser, Daniel E and Bachmann, Martin F
Journal: Frontiers in immunology (2019): 1015

Vaccination with nanoparticles combined with micro-adjuvants protects against cancer.
Authors: Mohsen, Mona O and Heath, Matthew D and Cabral-Miranda, Gustavo and Lipp, Cyrill and Zeltins, Andris and Sande, Marcos and Stein, Jens V and Riether, Carsten and Roesti, Elisa and Zha, Lisha and Engeroff, Paul and El-Turabi, Aadil and Kundig, Thomas M and Vogel, Monique and Skinner, Murray A and Speiser, Daniel E and Knuth, Alexander and Kramer, Matthias F and Bachmann, Martin F
Journal: Journal for immunotherapy of cancer (2019): 114

Direct Profiling the Post-Translational Modification Codes of a Single Protein Immobilized on a Surface Using Cu-free Click Chemistry.
Authors: Kim, Kyung Lock and Park, Kyeng Min and Murray, James and Kim, Kimoon and Ryu, Sung Ho
Journal: ACS central science (2018): 614-623

New Formulation for the Delivery of Oligonucleotides Using "Clickable" siRNA-Polyisoprenoid-Conjugated Nanoparticles: Application to Cancers Harboring Fusion Oncogenes.
Authors: Massaad-Massade, Liliane and Boutary, Suzan and Caillaud, Marie and Gracia, Celine and Parola, Beatrice and Gnaouiya, Soukaina Bel and Stella, Barbara and Arpicco, Silvia and Buchy, Eric and Desmaële, Didier and Couvreur, Patrick and Urbinati, Giorgia
Journal: Bioconjugate chemistry (2018): 1961-1972