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iFluor® 594 Tetrazine

The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. iFluor® 594 tetrazine can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis.
The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. iFluor® 594 tetrazine can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis.
Ordering information
Price ()
Catalog Number1018
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight1020.02
SolventDMSO
Spectral properties
Absorbance (nm)587
Correction Factor (260 nm)0.05
Correction Factor (280 nm)0.04
Extinction coefficient (cm -1 M -1)1800001
Excitation (nm)588
Emission (nm)604
Quantum yield0.531
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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iFluor® 680 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 700 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 750 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 790 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 350 goat anti-rabbit IgG (H+L)
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iFluor® 488 goat anti-rabbit IgG (H+L)
iFluor® 514 goat anti-rabbit IgG (H+L)
iFluor® 532 goat anti-rabbit IgG (H+L)
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iFluor® 594 goat anti-rabbit IgG (H+L)
iFluor® 633 goat anti-rabbit IgG (H+L)
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iFluor® 680 goat anti-rabbit IgG (H+L)
iFluor® 700 goat anti-rabbit IgG (H+L)
iFluor® 750 goat anti-rabbit IgG (H+L)
iFluor® 790 goat anti-rabbit IgG (H+L)
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iFluor® 700 Styramide *Superior Replacement for Alexa Fluor 700 tyramide*
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iFluor® 800 goat anti-rabbit IgG (H+L)
iFluor® 800 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 810 goat anti-rabbit IgG (H+L)
iFluor® 810 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 820 goat anti-rabbit IgG (H+L)
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iFluor® 840 goat anti-rabbit IgG (H+L)
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iFluor® 350 succinimidyl ester
iFluor® 405 succinimidyl ester
iFluor® 488 succinimidyl ester
iFluor® 514 succinimidyl ester
iFluor® 532 succinimidyl ester
iFluor® 555 succinimidyl ester
iFluor® 594 succinimidyl ester
iFluor® 633 succinimidyl ester
iFluor® 647 succinimidyl ester
iFluor® 660 succinimidyl ester
iFluor® 680 succinimidyl ester
iFluor® 700 succinimidyl ester
iFluor® 750 succinimidyl ester
iFluor® 610 succinimidyl ester
iFluor® 710 succinimidyl ester
iFluor® 790 succinimidyl ester
iFluor® 800 succinimidyl ester
iFluor® 810 succinimidyl ester
iFluor® 820 succinimidyl ester
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iFluor® 546 succinimidyl ester
iFluor® 568 succinimidyl ester
iFluor® 430 succinimidyl ester
iFluor® 450 succinimidyl ester
iFluor® 840 succinimidyl ester
iFluor® 560 succinimidyl ester
iFluor® 670 succinimidyl ester
iFluor® 460 succinimidyl ester
iFluor® 440 succinimidyl ester
iFluor® 665 succinimidyl ester
iFluor® 690 succinimidyl ester
iFluor® Ultra 594 succinimidyl ester
iFluor® Ultra 647 succinimidyl ester
iFluor® Ultra 750 succinimidyl ester
iFluor® 720 succinimidyl ester
iFluor® 740 succinimidyl ester
iFluor® 597 succinimidyl ester
iFluor® 770 succinimidyl ester
iFluor® 780 succinimidyl ester
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Show More (258)

OverviewpdfSDSpdfProtocol


Molecular weight
1020.02
Absorbance (nm)
587
Correction Factor (260 nm)
0.05
Correction Factor (280 nm)
0.04
Extinction coefficient (cm -1 M -1)
1800001
Excitation (nm)
588
Emission (nm)
604
Quantum yield
0.531
The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction. iFluor® 594 tetrazine can be readily used to label TCO-modified biological molecules for fluorescence imaging and other fluorescence-based biological applications. The conjugates prepared with iFluor® 594 dye have spectral properties almost identical to the popular Cy3.5 and Alexa Fluor® 594. In most cases, antibody conjugates prepared with iFluor® 594 tend to have lower background than the spectrally similar dye conjugates, such as Cy3.5 and Alexa Fluor® 594 (Alexa Fluor® is the trademark of Invitrogen).

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 594 Tetrazine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM98.037 µL490.186 µL980.373 µL4.902 mL9.804 mL
5 mM19.607 µL98.037 µL196.075 µL980.373 µL1.961 mL
10 mM9.804 µL49.019 µL98.037 µL490.186 µL980.373 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Absorbance (nm)587
Correction Factor (260 nm)0.05
Correction Factor (280 nm)0.04
Extinction coefficient (cm -1 M -1)1800001
Excitation (nm)588
Emission (nm)604
Quantum yield0.531

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 594 maleimide58860418000010.5310.050.04
iFluor® 594 Styramide *Superior Replacement for Alexa Fluor 594 tyramide*58860418000010.5310.050.04
iFluor® 594 Tyramide58860418000010.5310.050.04
iFluor® 594 TCO58860418000010.5310.050.04
iFluor® 488 Tetrazine4915167500010.910.210.11
iFluor® 555 Tetrazine55757010000010.6410.230.14
iFluor® 647 Tetrazine65667025000010.2510.030.03

References


View all 50 references: Citation Explorer
Facile Click-Mediated Cell Imaging Strategy of Liposomal Azido Mannosamine Lipids via Metabolic or Nonmetabolic Glycoengineering.
Authors: Shen, Li and Cai, Kaimin and Yu, Jin and Cheng, Jianjun
Journal: ACS omega (2020): 14111-14115
Bioorthogonal Labeling Reveals Different Expression of Glycans in Mouse Hippocampal Neuron Cultures during Their Development.
Authors: Soares da Costa, Diana and Sousa, João C and Dá Mesquita, Sandro and Petkova-Yankova, Nevena I and Marques, Fernanda and Reis, Rui L and Sousa, Nuno and Pashkuleva, Iva
Journal: Molecules (Basel, Switzerland) (2020)
Surface-modified nanoerythrosomes for potential optical imaging diagnostics.
Authors: Fornasier, Marco and Porcheddu, Andrea and Casu, Anna and Raghavan, Srinivasa R and Jönsson, Peter and Schillén, Karin and Murgia, Sergio
Journal: Journal of colloid and interface science (2020): 246-253
Novel Liposomal Azido Mannosamine Lipids on Metabolic Cell Labeling and Imaging via Cu-Free Click Chemistry.
Authors: Shen, Li and Cai, Kaimin and Yu, Jin and Cheng, Jianjun
Journal: Bioconjugate chemistry (2019): 2317-2322
One-Pot Synthesis of Triazolobenzodiazepines Through Decarboxylative [3 + 2] Cycloaddition of Nonstabilized Azomethine Ylides and Cu-Free Click Reactions.
Authors: Ma, Xiaoming and Zhang, Xiaofeng and Qiu, Weiqi and Zhang, Wensheng and Wan, Bruce and Evans, Jason and Zhang, Wei
Journal: Molecules (Basel, Switzerland) (2019)
Correction to "Photochemical Formation of Dibenzosilacyclohept-4-yne for Cu-Free Click Chemistry with Azides and 1,2,4,5-Tetrazines".
Authors: Martínek, Marek and Filipová, Lenka and Galeta, Juraj and Ludvíková, Lucie and Klán, Petr
Journal: Organic letters (2019): 8506
Targeting Mutated Plus Germline Epitopes Confers Pre-clinical Efficacy of an Instantly Formulated Cancer Nano-Vaccine.
Authors: Mohsen, Mona O and Vogel, Monique and Riether, Carsten and Muller, Julius and Salatino, Silvia and Ternette, Nicola and Gomes, Ariane C and Cabral-Miranda, Gustavo and El-Turabi, Aadil and Ruedl, Christiane and Kundig, Thomas M and Dermime, Said and Knuth, Alexander and Speiser, Daniel E and Bachmann, Martin F
Journal: Frontiers in immunology (2019): 1015
Vaccination with nanoparticles combined with micro-adjuvants protects against cancer.
Authors: Mohsen, Mona O and Heath, Matthew D and Cabral-Miranda, Gustavo and Lipp, Cyrill and Zeltins, Andris and Sande, Marcos and Stein, Jens V and Riether, Carsten and Roesti, Elisa and Zha, Lisha and Engeroff, Paul and El-Turabi, Aadil and Kundig, Thomas M and Vogel, Monique and Skinner, Murray A and Speiser, Daniel E and Knuth, Alexander and Kramer, Matthias F and Bachmann, Martin F
Journal: Journal for immunotherapy of cancer (2019): 114
Direct Profiling the Post-Translational Modification Codes of a Single Protein Immobilized on a Surface Using Cu-free Click Chemistry.
Authors: Kim, Kyung Lock and Park, Kyeng Min and Murray, James and Kim, Kimoon and Ryu, Sung Ho
Journal: ACS central science (2018): 614-623
New Formulation for the Delivery of Oligonucleotides Using "Clickable" siRNA-Polyisoprenoid-Conjugated Nanoparticles: Application to Cancers Harboring Fusion Oncogenes.
Authors: Massaad-Massade, Liliane and Boutary, Suzan and Caillaud, Marie and Gracia, Celine and Parola, Beatrice and Gnaouiya, Soukaina Bel and Stella, Barbara and Arpicco, Silvia and Buchy, Eric and Desmaële, Didier and Couvreur, Patrick and Urbinati, Giorgia
Journal: Bioconjugate chemistry (2018): 1961-1972