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iFluor® 594 Tetrazine
The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction. iFluor® 594 tetrazine can be readily used to label TCO-modified biological molecules for fluorescence imaging and other fluorescence-based biological applications. The conjugates prepared with iFluor® 594 dye have spectral properties almost identical to the popular Cy3.5 and Alexa Fluor® 594. In most cases, antibody conjugates prepared with iFluor® 594 tend to have lower background than the spectrally similar dye conjugates, such as Cy3.5 and Alexa Fluor® 594 (Alexa Fluor® is the trademark of Invitrogen).
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| iFluor® 488 Tetrazine | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
| iFluor® 555 Tetrazine | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| iFluor® 594 azide | 587 | 603 | 2000001 | 0.531 | 0.05 | 0.04 |
| iFluor® 594 maleimide | 587 | 603 | 2000001 | 0.531 | 0.05 | 0.04 |
| iFluor® 594 Styramide *Superior Replacement for Alexa Fluor 594 tyramide* | 587 | 603 | 2000001 | 0.531 | 0.05 | 0.04 |
| iFluor® 594 TCO | 587 | 603 | 2000001 | 0.531 | 0.05 | 0.04 |
| iFluor® 647 Tetrazine | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
References
View all 50 references: Citation Explorer
Facile Click-Mediated Cell Imaging Strategy of Liposomal Azido Mannosamine Lipids via Metabolic or Nonmetabolic Glycoengineering.
Authors: Shen, Li and Cai, Kaimin and Yu, Jin and Cheng, Jianjun
Journal: ACS omega (2020): 14111-14115
Authors: Shen, Li and Cai, Kaimin and Yu, Jin and Cheng, Jianjun
Journal: ACS omega (2020): 14111-14115
Bioorthogonal Labeling Reveals Different Expression of Glycans in Mouse Hippocampal Neuron Cultures during Their Development.
Authors: Soares da Costa, Diana and Sousa, João C and Dá Mesquita, Sandro and Petkova-Yankova, Nevena I and Marques, Fernanda and Reis, Rui L and Sousa, Nuno and Pashkuleva, Iva
Journal: Molecules (Basel, Switzerland) (2020)
Authors: Soares da Costa, Diana and Sousa, João C and Dá Mesquita, Sandro and Petkova-Yankova, Nevena I and Marques, Fernanda and Reis, Rui L and Sousa, Nuno and Pashkuleva, Iva
Journal: Molecules (Basel, Switzerland) (2020)
Surface-modified nanoerythrosomes for potential optical imaging diagnostics.
Authors: Fornasier, Marco and Porcheddu, Andrea and Casu, Anna and Raghavan, Srinivasa R and Jönsson, Peter and Schillén, Karin and Murgia, Sergio
Journal: Journal of colloid and interface science (2020): 246-253
Authors: Fornasier, Marco and Porcheddu, Andrea and Casu, Anna and Raghavan, Srinivasa R and Jönsson, Peter and Schillén, Karin and Murgia, Sergio
Journal: Journal of colloid and interface science (2020): 246-253
Novel Liposomal Azido Mannosamine Lipids on Metabolic Cell Labeling and Imaging via Cu-Free Click Chemistry.
Authors: Shen, Li and Cai, Kaimin and Yu, Jin and Cheng, Jianjun
Journal: Bioconjugate chemistry (2019): 2317-2322
Authors: Shen, Li and Cai, Kaimin and Yu, Jin and Cheng, Jianjun
Journal: Bioconjugate chemistry (2019): 2317-2322
One-Pot Synthesis of Triazolobenzodiazepines Through Decarboxylative [3 + 2] Cycloaddition of Nonstabilized Azomethine Ylides and Cu-Free Click Reactions.
Authors: Ma, Xiaoming and Zhang, Xiaofeng and Qiu, Weiqi and Zhang, Wensheng and Wan, Bruce and Evans, Jason and Zhang, Wei
Journal: Molecules (Basel, Switzerland) (2019)
Authors: Ma, Xiaoming and Zhang, Xiaofeng and Qiu, Weiqi and Zhang, Wensheng and Wan, Bruce and Evans, Jason and Zhang, Wei
Journal: Molecules (Basel, Switzerland) (2019)
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