iFluor® 594 TCO

The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. iFluor® 594-TCO can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis.

Ordering information

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	1045.11
Solvent	DMSO

Spectral properties

Absorbance (nm)	587
Correction Factor (260 nm)	0.05
Correction Factor (280 nm)	0.04
Extinction coefficient (cm ^-1 M ^-1)	180000¹
Excitation (nm)	588
Emission (nm)	604
Quantum yield	0.53¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

1045.11

Absorbance (nm)

587

Correction Factor (260 nm)

0.05

Correction Factor (280 nm)

0.04

Extinction coefficient (cm ^-1 M ^-1)

180000¹

Excitation (nm)

588

Emission (nm)

604

Quantum yield

0.53¹

The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction. iFluor® 594-TCO can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biological applications. The conjugates prepared with iFluor® 594 dye have spectral properties almost identical to the popular Cy3.5 and Alexa Fluor® 594. In most cases, antibody conjugates prepared with iFluor® 594 tend to have lower background than the spectrally similar dye conjugates, such as Cy3.5 and Alexa Fluor® 594 (Alexa Fluor® is the trademark of Invitrogen).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 594 TCO to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	95.684 µL	478.419 µL	956.837 µL	4.784 mL	9.568 mL
5 mM	19.137 µL	95.684 µL	191.367 µL	956.837 µL	1.914 mL
10 mM	9.568 µL	47.842 µL	95.684 µL	478.419 µL	956.837 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Absorbance (nm)	587
Correction Factor (260 nm)	0.05
Correction Factor (280 nm)	0.04
Extinction coefficient (cm ^-1 M ^-1)	180000¹
Excitation (nm)	588
Emission (nm)	604
Quantum yield	0.53¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 594 maleimide	588	604	180000¹	0.53¹	0.05	0.04
iFluor® 594 Styramide Superior Replacement for Alexa Fluor 594 tyramide	588	604	180000¹	0.53¹	0.05	0.04
iFluor® 594 Tyramide	588	604	180000¹	0.53¹	0.05	0.04
iFluor® 488 TCO	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 555 TCO	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 647 TCO	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 594 Tetrazine	588	604	180000¹	0.53¹	0.05	0.04

Images

Figure 1. The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. iFluor® 594-TCO can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis.

References

View all 33 references: Citation Explorer

Nanobody click chemistry for convenient site-specific fluorescent labelling, single step immunocytochemistry and delivery into living cells by photoporation and live cell imaging.
Authors: Hebbrecht, Tim and Liu, Jing and Zwaenepoel, Olivier and Boddin, Gaëlle and Van Leene, Chloé and Decoene, Klaas and Madder, Annemieke and Braeckmans, Kevin and Gettemans, Jan
Journal: New biotechnology (2020): 33-43

A novel tracer for in vivo optical imaging of fatty acid metabolism in the heart and brown adipose tissue.
Authors: Panagia, Marcello and Yang, Jing and Gale, Eric and Wang, Huan and Luptak, Ivan and Chen, Howard H and Patel, Dakshesh and Croteau, Dominique and Pimentel, David Richard and Bachschmid, Markus Michael and Colucci, Wilson S and Ran, Chongzhao and Sosnovik, David E
Journal: Scientific reports (2020): 11209

Tylophorine reduces protein biosynthesis and rapidly decreases cyclin D1, inhibiting vascular smooth muscle cell proliferation in vitro and in organ culture.
Authors: Joa, Helge and Blažević, Tina and Grojer, Christoph and Zeller, Iris and Heiss, Elke H and Atanasov, Atanas G and Feldler, Ines and Gruzdaitis, Päivi and Czaloun, Christa and Proksch, Peter and Messner, Barbara and Bernhard, David and Dirsch, Verena M
Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology (2019): 152938

PET imaging of EGFR expression using an 18F-labeled RNA aptamer.
Authors: Cheng, Siyuan and Jacobson, Orit and Zhu, Guizhi and Chen, Zhen and Liang, Steve H and Tian, Rui and Yang, Zhen and Niu, Gang and Zhu, Xiaohua and Chen, Xiaoyuan
Journal: European journal of nuclear medicine and molecular imaging (2019): 948-956

Long-term live cells observation of internalized fluorescent Fe@C nanoparticles in constant magnetic field.
Authors: Garanina, Anastasiia and Kireev, Igor and Zhironkina, Oxana and Strelkova, Olga and Shakhov, Anton and Alieva, Irina and Davydov, Valery and Murugesan, Sankaran and Khabashesku, Valery and Majouga, Alexander and Agafonov, Viatcheslav and Uzbekov, Rustem
Journal: Journal of nanobiotechnology (2019): 27

Bioorthogonal click chemistry for fluorescence imaging of choline phospholipids in plants.
Authors: Paper, Janet M and Mukherjee, Thiya and Schrick, Kathrin
Journal: Plant methods (2018): 31

Flow cytometry in formamide treated cells.
Authors: Radicchio, Giovanna and Colicchia, Valeria and Marrapodi, Ramona and Carbonari, Maurizio
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2018): 829-836

Intracellular in situ labeling of TiO2 nanoparticles for fluorescence microscopy detection.
Authors: Brown, Koshonna and Thurn, Ted and Xin, Lun and Liu, William and Bazak, Remon and Chen, Si and Lai, Barry and Vogt, Stefan and Jacobsen, Chris and Paunesku, Tatjana and Woloschak, Gayle E
Journal: Nano research (2018): 464-476

Fluorescent Labeling of Collagen Production by Cells for Noninvasive Imaging of Extracellular Matrix Deposition.
Authors: Bardsley, Katie and Yang, Ying and El Haj, Alicia J
Journal: Tissue engineering. Part C, Methods (2017): 228-236

Synthesis of 4-Azido-N-acetylhexosamine Uridine Diphosphate Donors: Clickable Glycosaminoglycans.
Authors: Zhang, Xing and Green, Dixy E and Schultz, Victor L and Lin, Lei and Han, Xiaorui and Wang, Ruitong and Yaksic, Alexander and Kim, So Young and DeAngelis, Paul L and Linhardt, Robert J
Journal: The Journal of organic chemistry (2017): 9910-9915