iFluor® 594 azide is a hydrophilic, red-emitting fluorescent dye engineered for efficient biomolecular labeling via click chemistry. It exhibits excitation and emission maxima at approximately 587 nm and 603 nm, respectively, aligning closely with dyes such as Texas Red® and Alexa Fluor® 594. A key advantage of iFluor® 594 is its pH-independent fluorescence, which remains stable across a broad pH range, ensuring consistent performance in different experimental conditions and improving reproducibility across diverse labeling applications.

The azide functionality of iFluor® 594 allows for precise and efficient bioconjugation with alkyne-functionalized molecules via copper-catalyzed azide-alkyne cycloaddition (CuAAC). Unlike conventional labeling strategies that modify amine or thiol groups, CuAAC provides exceptional chemoselectivity, minimizing nonspecific interactions and reducing background fluorescence. This improves labeling accuracy while maintaining the structural and functional integrity of biomolecules. iFluor® 594 azide is particularly suitable for applications including fluorescence microscopy, flow cytometry, and various fluorescence-based assays, providing researchers with a reliable tool for sensitive detection and imaging.