iFluor® 647 hydrazide
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 647 dyes have fluorescence excitation and emission maxima of ~656 nm and ~670 nm respectively. The iFluor® 647 family has spectral properties essentially identical to those of Cy5®. Compared to Cy5® probes, iFluor® 647 reagents have much stronger fluorescence and higher photostability. Their fluorescence is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family an excellent alternative to Cy5® and Alexa Fluor® 647 (Cy5® and Alexa Fluor® are the trademarks of GE Health Care and Invitrogen). iFluor® 647 hydrazide is reasonably stable and shows good reactivity and selectivity with a carbonyl group (such as aldehyde).
![Fluorescent dye hydrazine derivatives are the most popular tool for conjugating dyes to a target compound with a carbonyl group (e.g., aldehyde, carboxylic acid or activated carboxy group such as NHS ester). Fluorescent dye hydrazine derivatives are also useful for tracing neurons.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fifluor-647-hydrazide%2Ffigure-for-ifluor-647-hydrazide_8QZ2R.jpg&w=640&q=75)
![Fluorescent dye hydrazine derivatives are the most popular tool for conjugating dyes to a target compound with a carbonyl group (e.g., aldehyde, carboxylic acid or activated carboxy group such as NHS ester). Fluorescent dye hydrazine derivatives are also useful for tracing neurons.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fifluor-647-hydrazide%2Ffigure-for-ifluor-647-hydrazide_8QZ2R.jpg&w=640&q=75)
![Fluorescent dye hydrazine derivatives are the most popular tool for conjugating dyes to a target compound with a carbonyl group (e.g., aldehyde, carboxylic acid or activated carboxy group such as NHS ester). Fluorescent dye hydrazine derivatives are also useful for tracing neurons.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fifluor-647-hydrazide%2Ffigure-for-ifluor-647-hydrazide_8QZ2R.jpg&w=128&q=25)
Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 350 hydrazide | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
iFluor® 488 hydrazide | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 555 hydrazide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 680 hydrazide | 684 | 701 | 2200001 | 0.231 | 0.097 | 0.094 |
iFluor® 700 hydrazide | 690 | 713 | 2200001 | 0.231 | 0.09 | 0.04 |
iFluor® 750 hydrazide | 757 | 779 | 2750001 | 0.121 | 0.044 | 0.039 |
iFluor® 790 hydrazide | 787 | 812 | 2500001 | 0.131 | 0.1 | 0.09 |
iFluor® 405 hydrazide | 403 | 427 | 370001 | 0.911 | 0.48 | 0.77 |
iFluor® 647 Styramide *Superior Replacement for Alexa Fluor 647 tyramide* | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
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Citations
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Journal: (2024)
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Journal: (2024)
Development of nanobodies specific for clumping factors A of Staphylococcus aureus by yeast surface display
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Journal: International Journal of Biological Macromolecules (2024): 129208
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Journal: International Journal of Biological Macromolecules (2024): 129208
A Combinatorial Strategy for HRV 3C Protease Engineering to Achieve the N-terminal Free Cleavage
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Journal: bioRxiv (2024): 2024--01
Authors: Yi, Li and Zhang, Guimin and Mei, Meng and Fan, Xian and Zhou, Yu and Zhang, Faying
Journal: bioRxiv (2024): 2024--01
Generation of a cellular library of CAR T cells for rapid screening and identification of antigen specific, functional CAR constructs
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References
View all 49 references: Citation Explorer
Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
Authors: Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.
Journal: Zhen Ci Yan Jiu (2011): 262
Authors: Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.
Journal: Zhen Ci Yan Jiu (2011): 262
Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers
Authors: Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.
Journal: J Microbiol Methods (2011): 119
Authors: Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.
Journal: J Microbiol Methods (2011): 119
Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells
Authors: Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.
Journal: Biochem Biophys Res Commun (2011): 211
Authors: Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.
Journal: Biochem Biophys Res Commun (2011): 211
Visualizing dengue virus through Alexa Fluor labeling
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)
Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes
Authors: Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.
Journal: Mol Biosyst (2011): 1766
Authors: Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.
Journal: Mol Biosyst (2011): 1766