iFluor® 647 hydrazide
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 647 dyes have fluorescence excitation and emission maxima of ~656 nm and ~670 nm respectively. The iFluor® 647 family has spectral properties essentially identical to those of Cy5®. Compared to Cy5® probes, iFluor® 647 reagents have much stronger fluorescence and higher photostability. Their fluorescence is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family an excellent alternative to Cy5® and Alexa Fluor® 647 (Cy5® and Alexa Fluor® are the trademarks of GE Health Care and Invitrogen). iFluor® 647 hydrazide is reasonably stable and shows good reactivity and selectivity with a carbonyl group (such as aldehyde).
Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 350 hydrazide | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
iFluor® 488 hydrazide | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 555 hydrazide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 680 hydrazide | 684 | 701 | 2200001 | 0.231 | 0.097 | 0.094 |
iFluor® 700 hydrazide | 690 | 713 | 2200001 | 0.231 | 0.09 | 0.04 |
iFluor® 750 hydrazide | 757 | 779 | 2750001 | 0.121 | 0.044 | 0.039 |
iFluor® 790 hydrazide | 787 | 812 | 2500001 | 0.131 | 0.1 | 0.09 |
iFluor® 405 hydrazide | 403 | 427 | 370001 | 0.911 | 0.48 | 0.77 |
iFluor® 647 Styramide *Superior Replacement for Alexa Fluor 647 tyramide* | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
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Citations
View all 25 citations: Citation Explorer
Circ\_001653 alleviates sepsis associated-acute kidney injury by recruiting BUD13 to regulate KEAP1/NRF2/HO-1 signaling pathway
Authors: Li, Xinxin and Zhou, Wei and Chen, Jianjun and Zhou, Liangliang and Li, Yingbing and Wu, Xufeng and Peng, Xia
Journal: Journal of Inflammation (2024): 1--13
Authors: Li, Xinxin and Zhou, Wei and Chen, Jianjun and Zhou, Liangliang and Li, Yingbing and Wu, Xufeng and Peng, Xia
Journal: Journal of Inflammation (2024): 1--13
Novel immunogenic cell death inducer combined with autophagy inhibitor to amplify photodynamic synergistic immunotherapy for triple-negative breast cancer
Authors: Yuan, Gankun and Yang, Ruyue and Wen, Wenjing and Wei, Zhaoyi and Song, Meiru and Zhang, Lingyang and Hou, Kun and Liang, Gaofeng
Journal: (2024)
Authors: Yuan, Gankun and Yang, Ruyue and Wen, Wenjing and Wei, Zhaoyi and Song, Meiru and Zhang, Lingyang and Hou, Kun and Liang, Gaofeng
Journal: (2024)
The tandem CD33-CLL1 CAR-T as an approach to treat acute myeloid leukemia: The tandem CLL1/CD33 CAR-T to treat AML
Authors: Wang, Huiru and Feng, Shanglong and Zhu, Yanliang and Zhang, Yafeng and Zhou, Ziwei and Nian, Zhigang and Lu, Xueqin and Peng, Peng and Wu, Shu and Zhou, Li
Journal: Blood Transfusion (2024)
Authors: Wang, Huiru and Feng, Shanglong and Zhu, Yanliang and Zhang, Yafeng and Zhou, Ziwei and Nian, Zhigang and Lu, Xueqin and Peng, Peng and Wu, Shu and Zhou, Li
Journal: Blood Transfusion (2024)
Cryo-shocked tumor cells deliver CRISPR-Cas9 for lung cancer regression by synthetic lethality
Authors: Liu, Feng and Xin, Minhang and Feng, Huiheng and Zhang, Wentao and Liao, Ziyan and Sheng, Tao and Wen, Ping and Wu, Qing and Liang, Tingxizi and Shi, Jiaqi and others,
Journal: Science Advances (2024): eadk8264
Authors: Liu, Feng and Xin, Minhang and Feng, Huiheng and Zhang, Wentao and Liao, Ziyan and Sheng, Tao and Wen, Ping and Wu, Qing and Liang, Tingxizi and Shi, Jiaqi and others,
Journal: Science Advances (2024): eadk8264
Comparing the Malignant Properties of Parental and a knock-in version of HCT116 cell line expressing the CDK2-mutant of eukaryotic Elongation Factor 2 (eEF2)
Authors: Y{\"u}ksel, B{\"u}{\c{s}}ra and T{\"u}rkel, Nezaket and {\c{S}}ahin, Fikrettin and DENIZ, ASLI AYSEN HIZLI
Journal: (2024)
Authors: Y{\"u}ksel, B{\"u}{\c{s}}ra and T{\"u}rkel, Nezaket and {\c{S}}ahin, Fikrettin and DENIZ, ASLI AYSEN HIZLI
Journal: (2024)
References
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