iFluor® 790 hydrazide

In vivo fluorescence imaging uses a sensitive camera to detect the fluorescence emission from fluorophores in whole-body living small animals. To overcome the photon attenuation in living tissue, fluorophores with long emission at the near-infrared (NIR) region are generally preferred, including widely used small indocarbocyanine dyes. Recent advances in imaging strategies and reporter techniques for in vivo fluorescence imaging include novel approaches to improve the specificity and affinity of the probes and to modulate and amplify the signal at target sites for enhanced sensitivity. Further emerging developments aim to achieve high-resolution, multimodality, and lifetime-based in vivo fluorescence imaging. Our iFluor® 790 is designed to label proteins and other biomolecules with near-infrared fluorescence. Conjugates prepared with iFluor® 790 have excitation and emission spectra similar to that of indocyanine green (ICG) and the IRDye® 800, with 787/812 nm excitation/emission maxima. iFluor® 790 dye emission is well separated from commonly used far-red fluorophores such as Cy5, Cy7, or allophycocyanin (APC), facilitating multicolor analysis. This fluorophore is also useful for small animal in-vivo imaging applications or other imaging applications requiring NIR detections, such as the two-color western applications with the LI-COR® Odyssey® infrared imaging system.

<p>Fluorescent dye hydrazine derivatives are the most popular tool for conjugating dyes to a target compound with a carbonyl group (e.g., aldehyde, carboxylic acid or activated carboxy group such as NHS ester). Fluorescent dye hydrazine derivatives are also useful for tracing neurons.</p>

Protocol

SDS

COA

Catalog	Size	Price	Quantity
1364	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of iFluor® 790 hydrazide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	86.244 µL	431.22 µL	862.441 µL	4.312 mL	8.624 mL
5 mM	17.249 µL	86.244 µL	172.488 µL	862.441 µL	1.725 mL
10 mM	8.624 µL	43.122 µL	86.244 µL	431.22 µL	862.441 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1159.5 g/mol	=	mL	x	mM	=	mmol

References

View all 18 references: Citation Explorer

A target cell-specific activatable fluorescence probe for in vivo molecular imaging of cancer based on a self-quenched avidin-rhodamine conjugate

Authors:

Hama Y, Urano Y, Koyama Y, Kamiya M, Bernardo M, Paik RS, Shin IS, Paik CH, Choyke PL, Kobayashi H.

Journal:

Cancer Res (2007): 2791

Fluorescence imaging in vivo: recent advances

Authors:

Rao J, Dragulescu-Andrasi A, Yao H.

Journal:

Curr Opin Biotechnol (2007): 17

Ex vivo fluorescence imaging of normal and malignant urothelial cells to enhance early diagnosis

Authors:

Steenkeste K, Lecart S, Deniset A, Pernot P, Eschwege P, Ferlicot S, Leveque-Fort S, Bri and et R, Fontaine-Aupart MP.

Journal:

Photochem Photobiol (2007): 1157

In vivo monitoring the fate of Cy5.5-Tat labeled T lymphocytes by quantitative near-infrared fluorescence imaging during acute brain inflammation in a rat model of experimental autoimmune encephalomyelitis

Authors:

Berger C, Gremlich HU, Schmidt P, Cannet C, Kneuer R, Hiest and P, Rausch M, Rudin M.

Journal:

J Immunol Methods (2007): 65

A protocol for imaging alternative splicing regulation in vivo using fluorescence reporters in transgenic mice

Authors:

Bonano VI, Oltean S, Garcia-Blanco MA.

Journal:

Nat Protoc (2007): 2166

Physical properties

Molecular weight	1159.50
Solvent	Water

Spectral properties

Correction factor (260 nm)	0.1
Correction factor (280 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	250000 ¹
Excitation (nm)	787
Emission (nm)	812
Quantum yield	0.13 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Fluorescence microplate reader
Excitation	783 nm
Emission	814 nm
Cutoff	790 nm
Recommended plate	Solid black

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Page updated on October 8, 2025