iFluor® 488 hydrazide

Fluorescent dye hydrazine derivatives are the most popular tool for conjugating dyes to a target compound with a carbonyl group (e.g., aldehyde, carboxylic acid or activated carboxy group such as NHS ester). Fluorescent dye hydrazine derivatives are also useful for tracing neurons.

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

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Physical properties

Molecular weight	890.57
Solvent	Water

Spectral properties

Correction Factor (260 nm)	0.21
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Alternative formats

iFluor® 488 succinimidyl ester

iFluor® 488 amine

iFluor® 488 maleimide

iFluor® 488 azide

iFluor® 488 alkyne

Overview SDS Protocol

Molecular weight

890.57

Correction Factor (260 nm)

0.21

Correction Factor (280 nm)

0.11

Extinction coefficient (cm ^-1 M ^-1)

75000¹

Excitation (nm)

491

Emission (nm)

516

Quantum yield

0.9¹

Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with iFluor® 488 dyes are far superior to conjugates of fluorescein derivatives such as FITC. iFluor® 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor® 488 is not affected by pH (4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. iFluor® 488 hydrazide is reasonably stable and shows good reactivity and selectivity with a carbonyl group (such as aldehyde). This iFluor® 488 has spectral properties and reactivity similar to Alexa Fluor® 488 hydrazide ( Alexa Fluor® is the trademark of Invitrogen).

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of iFluor® 488 hydrazide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	112.288 µL	561.438 µL	1.123 mL	5.614 mL	11.229 mL
5 mM	22.458 µL	112.288 µL	224.575 µL	1.123 mL	2.246 mL
10 mM	11.229 µL	56.144 µL	112.288 µL	561.438 µL	1.123 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.21
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 350 hydrazide	345	450	20000¹	0.95¹	0.83	0.23
iFluor® 555 hydrazide	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 647 hydrazide	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 680 hydrazide	684	701	220000¹	0.23¹	0.097	0.094
iFluor® 700 hydrazide	690	713	220000¹	0.23¹	0.09	0.04
iFluor® 750 hydrazide	757	779	275000¹	0.12¹	0.044	0.039
iFluor® 790 hydrazide	787	812	250000¹	0.13¹	0.1	0.09
iFluor® 488 tyramide	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 405 hydrazide	403	427	37000¹	0.91¹	0.48	0.77
iFluor® 488 Styramide Superior Replacement for Alexa Fluor 488 tyramide and Opal 520	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 TCO	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 Tetrazine	491	516	75000¹	0.9¹	0.21	0.11
iFluor®488-dUTP 1 mM in Tris Buffer (pH 7.5)	491	516	75000¹	0.9¹	0.21	0.11
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Images

Figure 1. Fluorescent dye hydrazine derivatives are the most popular tool for conjugating dyes to a target compound with a carbonyl group (e.g., aldehyde, carboxylic acid or activated carboxy group such as NHS ester). Fluorescent dye hydrazine derivatives are also useful for tracing neurons.

Citations

View all 14 citations: Citation Explorer

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Authors: Wang, Dan and Qu, Hao and Kang, Hui and Xu, Feng and Huang, Wei and Cai, Xianhua
Journal: Bioengineered (2022): 8772--8784

Independent phenotypic plasticity axes define distinct obesity sub-types
Authors: Yang, Chih-Hsiang and Fagnocchi, Luca and Apostle, Stefanos and Wegert, Vanessa and Casan{\'\i}-Gald{\'o}n, Salvador and Landgraf, Kathrin and Panzeri, Ilaria and Dror, Erez and Heyne, Steffen and W{\"o}rpel, Till and others,
Journal: Nature metabolism (2022): 1150--1165

ZBTB7A promotes virus-host homeostasis during human coronavirus 229E infection
Authors: Zhu, Xinyu and Trimarco, Joseph D and Williams, Courtney A and Barrera, Alejandro and Reddy, Timothy E and Heaton, Nicholas S
Journal: Cell Reports (2022): 111540

Osteoclasts directly influence castration-resistant prostate cancer cells
Authors: Huang, Junchi and Freyhult, Eva and Buckland, Robert and Josefsson, Andreas and Damber, Jan-Erik and Wel{\'e}n, Karin
Journal: Clinical \& experimental metastasis (2022): 801--814

A Novel CDK4/6 and PARP Dual Inhibitor ZC-22 Effectively Suppresses Tumor Growth and Improves the Response to Cisplatin Treatment in Breast and Ovarian Cancer
Authors: Tian, Chenchen and Wei, Yufan and Li, Jianjun and Huang, Zhi and Wang, Qiong and Lin, Yingxue and Lv, Xingping and Chen, Yanan and Fan, Yan and Sun, Peiqing and others,
Journal: International journal of molecular sciences (2022): 2892

Adenosine signaling activates ATP-sensitive K+ channels in endothelial cells and pericytes in CNS capillaries
Authors: Sancho, Maria and Klug, Nicholas R and Mughal, Amreen and Koide, Masayo and Huerta de la Cruz, Saul and Heppner, Thomas J and Bonev, Adrian D and Hill-Eubanks, David and Nelson, Mark T
Journal: Science Signaling (2022): eabl5405

Endogenous glutamine is rate-limiting for anti-Cd3 and anti-Cd28 induced Cd4+ T-cell proliferation and glycolytic activity under hypoxia and normoxia
Authors: Wik, Jonas A and Chowdhury, Azazul and Kolan, Shrikant and Bastani, Nasser E and Li, Gaoyang and Alam, Kazi and Grimolizzi, Franco and Sk{\aa}lhegg, Bj{\o}rn S
Journal: Biochemical Journal (2022): 1221--1235

Regulation of circADAMTS6-miR-324-5p-PIK3R3 ceRNA pathway may be a novel mechanism of IL-1$\beta$-induced osteoarthritic chondrocytes
Authors: Shen, Lanjuan and Ji, Cheng and Lin, Jian and Yang, Hongping
Journal: Journal of Bone and Mineral Metabolism (2022): 389--401

Silencing of Circ\_0135889 Restrains Proliferation and Tumorigenicity of Human Neuroblastoma Cells
Authors: Yang, Jun and Liu, Bao and Xu, Zhenli and Feng, Mei
Journal: Journal of Surgical Research (2022): 135--147

CD95/Fas protects triple negative breast cancer from anti-tumor activity of NK cells
Authors: Qadir, Abdul S and Gu{\'e}gan, Jean Philippe and Ginestier, Christophe and Chaibi, Assia and Bessede, Alban and Charafe-Jauffret, Emmanuelle and Macario, Manon and Lavou{\'e}, Vincent and de la Motte Rouge, Thibault and Law, Calvin and others,
Journal: Iscience (2021): 103348

References

View all 49 references: Citation Explorer

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