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AAT Bioquest

iFluor® 700 hydrazide

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 700 dyes have fluorescence excitation and emission maxima of ~690 nm and ~713 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 700 labeling dyes (Alexa Fluor® is the trademark of Invitrogen). iFluor® 700 hydrazide is reasonably stable and shows good reactivity and selectivity with a carbonyl group (such as aldehyde).

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of iFluor® 700 hydrazide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM97.946 µL489.73 µL979.461 µL4.897 mL9.795 mL
5 mM19.589 µL97.946 µL195.892 µL979.461 µL1.959 mL
10 mM9.795 µL48.973 µL97.946 µL489.73 µL979.461 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 350 hydrazide3454502000010.9510.830.23
iFluor® 488 hydrazide4915167500010.910.210.11
iFluor® 555 hydrazide55757010000010.6410.230.14
iFluor® 647 hydrazide65667025000010.2510.030.03
iFluor® 680 hydrazide68470122000010.2310.0970.094
iFluor® 750 hydrazide75777927500010.1210.0440.039
iFluor® 790 hydrazide78781225000010.1310.10.09
iFluor® 405 hydrazide4034273700010.9110.480.77
iFluor® 700 Styramide *Superior Replacement for Alexa Fluor 700 tyramide*69071322000010.2310.090.04
PerCP-iFluor® 700675707350000---
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Citations

View all 3 citations: Citation Explorer
Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification
Authors: Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined
Journal: Metabolomics (Los Angel) (2017): 2153--0769
Suramin inhibits cullin-RING E3 ubiquitin ligases
Authors: Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined
Journal: Proceedings of the National Academy of Sciences (2016): E2011--E2018
Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function
Authors: Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined
Journal: International Journal of Cancer (2012): E425--E436

References

View all 49 references: Citation Explorer
Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes
Authors: Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.
Journal: Mol Biosyst (2011): 1766
Visualizing dengue virus through Alexa Fluor labeling
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)
Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells
Authors: Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.
Journal: Biochem Biophys Res Commun (2011): 211
Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
Authors: Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.
Journal: Zhen Ci Yan Jiu (2011): 262
Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers
Authors: Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.
Journal: J Microbiol Methods (2011): 119
Page updated on December 11, 2024

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Catalog Number1087
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Physical properties

Molecular weight

1020.97

Solvent

Water

Spectral properties

Correction Factor (260 nm)

0.09

Correction Factor (280 nm)

0.04

Correction Factor (565 nm)

0.0766

Correction Factor (650 nm)

0.4004

Extinction coefficient (cm -1 M -1)

2200001

Excitation (nm)

690

Emission (nm)

713

Quantum yield

0.231

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Flow cytometric analysis of APC-iFluor®700&nbsp; (Red Bar) or APC-Alexa Fluor&reg; 700&nbsp; (Blue Bar)&nbsp; anti-human CD8 on human lymphocytes. Whole blood was stained with APC-iFluor®700&nbsp; or&nbsp; APC-Alexa Fluor&reg; 700&nbsp; anti-human CD8&nbsp; and compared to whole blood stained with a APC-iFluor®700 and APC-Alexa Fluor&reg; 700 mouse IgG control. Flow cytometry was performed on a ACEA flow cytometry system.
Flow cytometric analysis of APC-iFluor®700&nbsp; (Red Bar) or APC-Alexa Fluor&reg; 700&nbsp; (Blue Bar)&nbsp; anti-human CD8 on human lymphocytes. Whole blood was stained with APC-iFluor®700&nbsp; or&nbsp; APC-Alexa Fluor&reg; 700&nbsp; anti-human CD8&nbsp; and compared to whole blood stained with a APC-iFluor®700 and APC-Alexa Fluor&reg; 700 mouse IgG control. Flow cytometry was performed on a ACEA flow cytometry system.
Flow cytometric analysis of APC-iFluor®700&nbsp; (Red Bar) or APC-Alexa Fluor&reg; 700&nbsp; (Blue Bar)&nbsp; anti-human CD8 on human lymphocytes. Whole blood was stained with APC-iFluor®700&nbsp; or&nbsp; APC-Alexa Fluor&reg; 700&nbsp; anti-human CD8&nbsp; and compared to whole blood stained with a APC-iFluor®700 and APC-Alexa Fluor&reg; 700 mouse IgG control. Flow cytometry was performed on a ACEA flow cytometry system.