iFluor®488-dUTP 1 mM in TE Buffer (pH 7.5)

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	1152.83
Solvent	Water

Spectral properties

Correction Factor (260 nm)	0.21
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

1152.83

Correction Factor (260 nm)

0.21

Correction Factor (280 nm)

0.11

Extinction coefficient (cm ^-1 M ^-1)

75000¹

Excitation (nm)

491

Emission (nm)

516

Quantum yield

0.9¹

The dye-modified deoxyuridine 5'-triphosphates are one of the most common methods to produce dye-labelled DNA via the conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This iFluor 488-dUTP conjugate is used as a green fluorescence color with SpectrumGreen™ filter set (SpectrumGreen™ is the trademark of Vysis). Compared to the commonly used green color probes (e.g., Fluorescein-12-dUTP), iFluor 488 provides much brighter signal that has much photostability and is not affected by pH.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of iFluor®488-dUTP *1 mM in TE Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	86.743 µL	433.715 µL	867.431 µL	4.337 mL	8.674 mL
5 mM	17.349 µL	86.743 µL	173.486 µL	867.431 µL	1.735 mL
10 mM	8.674 µL	43.372 µL	86.743 µL	433.715 µL	867.431 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.21
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 488 maleimide	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 amine	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 hydrazide	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 tyramide	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 azide	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 alkyne	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 Styramide Superior Replacement for Alexa Fluor 488 tyramide and Opal 520	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 TCO	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 Tetrazine	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 440-dUTP 1 mM in TE Buffer (pH 7.5)	434	480	40000¹	0.67¹	0.352	0.229
iFluor® 560-dUTP 1 mM in TE Buffer (pH 7.5)	560	571	120000¹	0.57¹	0.0482	0.069
XFD™488-dUTP 1 mM in TE Buffer (pH 7.5)	499	520	73000	0.92¹	0.3	0.11
Show More (3)

Images

Figure 1. Chemical structure for iFluor®488-dUTP *1 mM in TE Buffer (pH 7.5)*.

Figure 2. Fluorescence In Situ Hybridization of Fluorescein and iFluor® 488-dUTP labelled Telomere probes in metaphase HeLa cells.

References

View all 23 references: Citation Explorer

Anti-proliferative effect of Fe(III) complexed with 1-(2-hydroxy-3-methoxybenzaldehyde)-4-aminosalicylhydrazone in HepG2 cells.
Authors: Fukushima, Takeshi and Taniguchi, Erina and Yamada, Hiroshi and Kato, Kiyomasa and Shimizu, Ayako and Nishiguchi, Yoshikazu and Onozato, Mayu and Ichiba, Hideaki and Azuma, Yutaro
Journal: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine (2015): 669-77

Localisation of abundant and organ-specific genes expressed in Rosa hybrida leaves and flower buds by direct in situ RT-PCR.
Authors: Jedrzejuk, Agata and Mibus, Heiko and Serek, Margrethe
Journal: TheScientificWorldJournal (2012): 609597

SNP genotyping using microsphere-linked PNA and flow cytometric detection.
Authors: Rockenbauer, Eszter and Petersen, Kenneth and Vogel, Ulla and Bolund, Lars and Kølvraa, Steen and Nielsen, Kirsten Vang and Nexø, Bjørn A
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2005): 80-6

[Comparative genomic hybridization analysis of nasopharyngeal carcinoma drug-resistant cell CNE2/DDP and its parent cell CNE2].
Authors: Jiang, Run-De and Hu, Liang and Guan, Xin-Yuan and Zhang, Li-Xin and Yue, Wen and Cen, Xin-Tang and Li, Chun-Hai
Journal: Ai zheng = Aizheng = Chinese journal of cancer (2004): 386-90

Flow cytometric detection of beta-D-glucuronidase gene in wild-type bacterial cells using in-situ PCR.
Authors: Sachidanandham, Ramaiah and Gin, Karina Yew-Hoong
Journal: Biotechnology and bioengineering (2003): 127-33

Lack of nuclear apoptosis in cardiomyocytes and increased endothelin-1 levels in a rat heart model of myocardial stunning.
Authors: Klainguti, M and Aigner, S and Kilo, J and Eppenberger, H M and Mandinova, A and Aebi, U and Schaub, M C and Shaw, S G and Lüscher, T F and Atar, D
Journal: Basic research in cardiology (2000): 308-15

A method to monitor DNA transfer during transfection.
Authors: Johnson, A L and Jurcisek, J A and Trask, O J and Au, J L
Journal: AAPS pharmSci (1999): E6

Pulsatile shear stress leads to DNA fragmentation in human SH-SY5Y neuroblastoma cell line.
Authors: Triyoso, D H and Good, T A
Journal: The Journal of physiology (1999): 355-65

Detection of TNF alpha and Fas ligand mRNA within synovial mononuclear cells by fluorescence in-cell labeling PCR (FICL-PCR).
Authors: Okubo, M and Brown, M P and Chiba, K and Kasukawa, R and Nishimaki, T
Journal: Molecular biology reports (1998): 217-24

Targeted nucleotide exchange in the alkaline phosphatase gene of HuH-7 cells mediated by a chimeric RNA/DNA oligonucleotide.
Authors: Kren, B T and Cole-Strauss, A and Kmiec, E B and Steer, C J
Journal: Hepatology (Baltimore, Md.) (1997): 1462-8