iFluor®488-dUTP *1 mM in TE Buffer (pH 7.5)*

iFluor®488-dUTP

1 mM in TE Buffer (pH 7.5)

The dye-modified deoxyuridine 5'-triphosphates are one of the most common methods to produce dye-labelled DNA via the conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This iFluor® 488-dUTP conjugate is used as a green fluorescence color with SpectrumGreen™ filter set (SpectrumGreen™ is the trademark of Vysis). Compared to the commonly used green color probes (e.g., Fluorescein-12-dUTP), iFluor® 488 provides much brighter signal that has much photostability and is not affected by pH.

Protocol

SDS

Catalog	Size	Price	Quantity
17039	25 nmoles	Price
17044	100 nmoles	Price

References

View all 23 references: Citation Explorer

Anti-proliferative effect of Fe(III) complexed with 1-(2-hydroxy-3-methoxybenzaldehyde)-4-aminosalicylhydrazone in HepG2 cells.

Authors:

Fukushima, Takeshi and Taniguchi, Erina and Yamada, Hiroshi and Kato, Kiyomasa and Shimizu, Ayako and Nishiguchi, Yoshikazu and Onozato, Mayu and Ichiba, Hideaki and Azuma, Yutaro

Journal:

Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine (2015): 669-77

Localisation of abundant and organ-specific genes expressed in Rosa hybrida leaves and flower buds by direct in situ RT-PCR.

Authors:

Jedrzejuk, Agata and Mibus, Heiko and Serek, Margrethe

Journal:

TheScientificWorldJournal (2012): 609597

SNP genotyping using microsphere-linked PNA and flow cytometric detection.

Authors:

Rockenbauer, Eszter and Petersen, Kenneth and Vogel, Ulla and Bolund, Lars and Kølvraa, Steen and Nielsen, Kirsten Vang and Nexø, Bjørn A

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2005): 80-6

[Comparative genomic hybridization analysis of nasopharyngeal carcinoma drug-resistant cell CNE2/DDP and its parent cell CNE2].

Authors:

Jiang, Run-De and Hu, Liang and Guan, Xin-Yuan and Zhang, Li-Xin and Yue, Wen and Cen, Xin-Tang and Li, Chun-Hai

Journal:

Ai zheng = Aizheng = Chinese journal of cancer (2004): 386-90

Flow cytometric detection of beta-D-glucuronidase gene in wild-type bacterial cells using in-situ PCR.

Authors:

Sachidanandham, Ramaiah and Gin, Karina Yew-Hoong

Journal:

Biotechnology and bioengineering (2003): 127-33

Physical properties

Molecular weight	1152.83
Solvent	Water

Spectral properties

Correction factor (260 nm)	0.21
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Flyer: Labeled dUTPs

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Page updated on October 30, 2025