iFluor® 405 hydrazide

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 350 dyes have fluorescence excitation and emission maxima of ~403 nm and ~427 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 405 labeling dye (Alexa Fluor® is the trademark of Invitrogen). iFluor® 405 hydrazide is stable and shows good reactivity and selectivity with a carbonyl group (such as aldehyde).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 405 hydrazide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	148.69 µL	743.45 µL	1.487 mL	7.435 mL	14.869 mL
5 mM	29.738 µL	148.69 µL	297.38 µL	1.487 mL	2.974 mL
10 mM	14.869 µL	74.345 µL	148.69 µL	743.45 µL	1.487 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 350 hydrazide	345	450	20000¹	0.95¹	0.83	0.23
iFluor® 488 hydrazide	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 555 hydrazide	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 647 hydrazide	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 680 hydrazide	684	701	220000¹	0.23¹	0.097	0.094
iFluor® 700 hydrazide	690	713	220000¹	0.23¹	0.09	0.04
iFluor® 750 hydrazide	757	779	275000¹	0.12¹	0.044	0.039
iFluor® 790 hydrazide	787	812	250000¹	0.13¹	0.1	0.09
iFluor® 405 Styramide	403	427	37000¹	0.91¹	0.48	0.77

Citations

View all 4 citations: Citation Explorer

PF-D-Trimer, a protective SARS-CoV-2 subunit vaccine: immunogenicity and application
Authors: Zhang, Zhihao and Zhou, Jinhu and Ni, Peng and Hu, Bing and Jolicoeur, Normand and Deng, Shuang and Xiao, Qian and He, Qian and Li, Gai and Xia, Yan and others,
Journal: npj Vaccines (2023): 38

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification
Authors: Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined
Journal: Metabolomics (Los Angel) (2017): 2153--0769

Suramin inhibits cullin-RING E3 ubiquitin ligases
Authors: Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined
Journal: Proceedings of the National Academy of Sciences (2016): E2011--E2018

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function
Authors: Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined
Journal: International Journal of Cancer (2012): E425--E436

References

View all 49 references: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes
Authors: Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.
Journal: Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells
Authors: Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.
Journal: Biochem Biophys Res Commun (2011): 211

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
Authors: Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.
Journal: Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers
Authors: Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.
Journal: J Microbiol Methods (2011): 119

Page updated on September 18, 2024

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Catalog Number	1081
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Physical properties

Molecular weight	672.54
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.48
Correction Factor (280 nm)	0.77
Extinction coefficient (cm ^-1 M ^-1)	37000¹
Excitation (nm)	403
Emission (nm)	427
Quantum yield	0.91¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501