iFluor® 488 TCO

The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction. iFluor® 488-TCO can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. The conjugates prepared with iFluor® 488 dyes are far superior to those of fluorescein derivatives such as FITC. iFluor® 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor® 488 is not affected by pH (4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. iFluor® 488 has spectral properties similar to Alexa Fluor® 488. In some cases, it demonstrates brighter signals (Alexa Fluor® is the trademark of Invitrogen).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
1005	1 mg	Price

Citations

View all 2 citations: Citation Explorer

Vibrational Markers of a Model Circulating Metastatic Cells LLC-R9

Authors:

Gnatyuk, Olena and Kolesnyk, Denys and Voitsitskyi, Taras and Karakhim, Sergiy and Nikolenko, Andriy and Dementjev, Andrej and Solyanik, Galina and Dovbeshko, Galyna

Journal:

Spectroscopy Journal (2024): 306--321

CD95/Fas protects triple negative breast cancer from anti-tumor activity of NK cells

Authors:

Qadir, Abdul S and Gu{\'e}gan, Jean Philippe and Ginestier, Christophe and Chaibi, Assia and Bessede, Alban and Charafe-Jauffret, Emmanuelle and Macario, Manon and Lavou{\'e}, Vincent and de la Motte Rouge, Thibault and Law, Calvin and others,

Journal:

Iscience (2021): 103348

References

View all 50 references: Citation Explorer

Clickable modular polysaccharide nanoparticles for selective cell-targeting.

Authors:

Peuler, Kevin and Dimmitt, Nathan and Lin, Chien-Chi

Journal:

Carbohydrate polymers (2020): 115901

A Systematic Study of Coumarin-Tetrazine Light-Up Probes for Bioorthogonal Fluorescence Imaging.

Authors:

Galeta, Juraj and Dzijak, Rastislav and Obořil, Jan and Dračínský, Martin and Vrabel, Milan

Journal:

Chemistry (Weinheim an der Bergstrasse, Germany) (2020)

Facile Discovery and Quantification of Isonitrile Natural Products via Tetrazine-Based Click Reactions.

Authors:

Huang, Yao-Bing and Cai, Wenlong and Del Rio Flores, Antonio and Twigg, Frederick F and Zhang, Wenjun

Journal:

Analytical chemistry (2020): 599-602

In Vivo Imaging of Click-Crosslinked Hydrogel Depots Following Intratympanic Injection.

Authors:

Ju, Hyeon Jin and Park, Mina and Park, Ji Hoon and Shin, Gi Ru and Choi, Hak Soo and Suh, Myung-Whan and Kim, Moon Suk

Journal:

Materials (Basel, Switzerland) (2020)

Design, Synthesis, Conjugation, and Reactivity of Novel trans,trans-1,5-Cyclooctadiene-Derived Bioorthogonal Linkers.

Authors:

Longo, Beatrice and Zanato, Chiara and Piras, Monica and Dall'Angelo, Sergio and Windhorst, Albert D and Vugts, Danielle J and Baldassarre, Massimiliano and Zanda, Matteo

Journal:

Bioconjugate chemistry (2020)

Physical properties

Molecular weight	945.16
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.21
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Shipping	Standard overnight for United States, inquire for international

Page updated on October 13, 2025