iFluor® 488 TCO

The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. iFluor® 488-TCO can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis.

Ordering information

Price
Catalog Number
Unit Size
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	945.16
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.21
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

945.16

Correction Factor (260 nm)

0.21

Correction Factor (280 nm)

0.11

Extinction coefficient (cm ^-1 M ^-1)

75000¹

Excitation (nm)

491

Emission (nm)

516

Quantum yield

0.9¹

The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction. iFluor® 488-TCO can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. The conjugates prepared with iFluor® 488 dyes are far superior to those of fluorescein derivatives such as FITC. iFluor® 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor® 488 is not affected by pH (4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. iFluor® 488 has spectral properties similar to Alexa Fluor® 488. In some cases, it demonstrates brighter signals (Alexa Fluor® is the trademark of Invitrogen).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 488 TCO to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	105.802 µL	529.011 µL	1.058 mL	5.29 mL	10.58 mL
5 mM	21.16 µL	105.802 µL	211.604 µL	1.058 mL	2.116 mL
10 mM	10.58 µL	52.901 µL	105.802 µL	529.011 µL	1.058 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.21
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 488 maleimide	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 amine	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 hydrazide	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 tyramide	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 azide	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 alkyne	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 Styramide Superior Replacement for Alexa Fluor 488 tyramide and Opal 520	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 555 TCO	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 594 TCO	588	604	180000¹	0.53¹	0.05	0.04
iFluor® 647 TCO	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 488 Tetrazine	491	516	75000¹	0.9¹	0.21	0.11
iFluor®488-dUTP 1 mM in Tris Buffer (pH 7.5)	491	516	75000¹	0.9¹	0.21	0.11
Show More (3)

Images

Figure 1. The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. iFluor® 488-TCO can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis.

Citations

View all 1 citations: Citation Explorer

CD95/Fas protects triple negative breast cancer from anti-tumor activity of NK cells
Authors: Qadir, Abdul S and Gu{\'e}gan, Jean Philippe and Ginestier, Christophe and Chaibi, Assia and Bessede, Alban and Charafe-Jauffret, Emmanuelle and Macario, Manon and Lavou{\'e}, Vincent and de la Motte Rouge, Thibault and Law, Calvin and others,
Journal: Iscience (2021): 103348

References

View all 50 references: Citation Explorer

Fluorescent bioorthogonal labeling of class B GPCRs in live cells.
Authors: Gangam, Srikanth Kumar and Lin, Qing
Journal: Methods in enzymology (2020): 95-111

Bond-Breaking Bio-orthogonal Chemistry Efficiently Uncages Fluorescent and Therapeutic Compounds under Physiological Conditions.
Authors: Wu, Xunshen and Wu, Kui and Gaye, Fatima and Royzen, Maksim
Journal: Organic letters (2020): 6041-6044

Fluorescent Janus emulsions for biosensing of Listeria monocytogenes.
Authors: Li, Jie and Savagatrup, Suchol and Nelson, Zachary and Yoshinaga, Kosuke and Swager, Timothy M
Journal: Proceedings of the National Academy of Sciences of the United States of America (2020): 11923-11930

Transition-Metal-Mediated versus Tetrazine-Triggered Bioorthogonal Release Reactions: Direct Comparison and Combinations Thereof.
Authors: Mancuso, Francesca and Rahm, Michal and Dzijak, Rastislav and Mertlíková-Kaiserová, Helena and Vrabel, Milan
Journal: ChemPlusChem (2020): 1669-1675

Conditionally Controlling Human TLR2 Activity via Trans-Cyclooctene Caged Ligands.
Authors: van de Graaff, Michel J and Oosenbrug, Timo and Marqvorsen, Mikkel H S and Nascimento, Clarissa R and de Geus, Mark A R and Manoury, Bénédicte and Ressing, Maaike E and van Kasteren, Sander I
Journal: Bioconjugate chemistry (2020): 1685-1692

Click by Click Microporous Annealed Particle (MAP) Scaffolds.
Authors: Darling, Nicole J and Xi, Weixian and Sideris, Elias and Anderson, Alexa R and Pong, Cassie and Carmichael, S Thomas and Segura, Tatiana
Journal: Advanced healthcare materials (2020): e1901391

Hydrogel Synthesis and Stabilization via Tetrazine Click-Induced Secondary Interactions.
Authors: Holt, Samantha E and Rakoski, Amanda and Jivan, Faraz and Pérez, Lisa M and Alge, Daniel L
Journal: Macromolecular rapid communications (2020): e2000287

Cellular Delivery of Bioorthogonal Pretargeting Therapeutics in PSMA-Positive Prostate Cancer.
Authors: Hapuarachchige, Sudath and Huang, Colin T and Donnelly, Madeline C and Bařinka, Cyril and Lupold, Shawn E and Pomper, Martin G and Artemov, Dmitri
Journal: Molecular pharmaceutics (2020): 98-108

Ultra-fast Cycling for Multiplexed Cellular Fluorescence Imaging.
Authors: Ko, Jina and Oh, Juhyun and Ahmed, Maaz S and Carlson, Jonathan C T and Weissleder, Ralph
Journal: Angewandte Chemie (International ed. in English) (2020): 6839-6846

A General Strategy for Macrotheranostic Prodrug Activation: Synergy between the Acidic Tumor Microenvironment and Bioorthogonal Chemistry.
Authors: Dong, Yansong and Tu, Yalan and Wang, Kewei and Xu, Congfei and Yuan, Youyong and Wang, Jun
Journal: Angewandte Chemie (International ed. in English) (2020): 7168-7172