iFluor® 488 azide

iFluor® 488 Azide is a green-fluorescent, click chemistry-reactive dye for covalent labeling of alkyne-modified biomolecules in fluorescence-based research applications.

**Azide Functionality: Selectively reacts with alkyne groups through click chemistry.
Spectral Properties: Excitation at 491 nm and emission at 516 nm.
Fluorescence Performance: Bright, photostable fluorescence with a quantum yield of 0.90.
pH Sensitivity: Maintains stable fluorescence across pH 4–10.
Applications: Suitable for fluorescent labeling of alkyne-functionalized molecules and biomolecular conjugates.

<p>Click chemistry is a method for attaching a probe or substrate of interest to a specific biomolecule, a process called bioconjugation. The possibility of attaching fluorophores and other reporter molecules has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an azide with an alkyne to form a 5-membered heteroatom ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC).</p>

Datasheet

SDS

Catalog	Size	Price	Quantity
1000	1 mg	Price

Overview

Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with iFluor® 488 dyes are far superior to conjugates of fluorescein derivatives such as FITC. iFluor® 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor® 488 is not affected by pH (4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. iFluor® 488 azide dye is reasonably stable and shows good reactivity and selectivity with the alkyne group for click chemistry applications. The iFluor® 488 dyes are an excellent replacement to the Alexa Fluor dyes that have identical spectral properties ( Alexa Fluor® is the trademark of Invitrogen).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 488 azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	139.554 µL	697.769 µL	1.396 mL	6.978 mL	13.955 mL
5 mM	27.911 µL	139.554 µL	279.107 µL	1.396 mL	2.791 mL
10 mM	13.955 µL	69.777 µL	139.554 µL	697.769 µL	1.396 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	716.57 g/mol	=	mL	x	mM	=	mmol

Citations

View all 51 citations: Citation Explorer

The teneurin TEN-1 and its ligand LRON-11 support microbiota-induced neuroprotection and mechanosensory function in C. elegans

Authors:

Li, Jingxian and Castillo, Juan Pablo and Cheng, Shouqiang and Weathers, Indya and Nawrocka, Wioletta I and Alvarado, Jorge and Zhao, Minglei and Calixto, Andrea and {\"O}zkan, Engin and Ara{\c{c}}, Demet

Journal:

Current Biology (2026)

Carnosol promotes myogenesis in livestock muscle stem cells through coordinated regulation of myostatin signaling and mitochondrial MSS51

Authors:

Lim, Jeong Ho and Shaikh, Sibhghatulla and Lee, Eun Ju and Choi, Inho

Journal:

Food Bioscience (2026): 109987

A Signal Peptide Screening Approach for Improved Fc-Fusion Expression in CHO-S Cells

Authors:

Holm, Isak

Developing mouse inhibitory neuron single-cell transcriptomes reveal distinct modes of cell-type diversification

Authors:

Liu, Minhui and Restelli, Facundo Ferrero and Micoli, Elia and Barbiera, Giulia and Moors, Rani and Nouboers, Evelien and Reverendo, Malou and Du, Jessica Xinyun and Bertels, Hannah and Konstantopoulos, Dimitris and others,

Journal:

Nature Neuroscience (2026): 1--13

Fully Human Antagonistic Antibodies Targeting FPR2 Through Dual Extracellular-Loop Engagement for Gastric Cancer Therapy

Authors:

Kim, Min Su and Kang, Tae Hyun

Journal:

Antibody Therapeutics (2026): tbag035

References

View all 49 references: Citation Explorer

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Authors:

Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.

Journal:

Zhen Ci Yan Jiu (2011): 262

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells

Authors:

Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.

Journal:

Biochem Biophys Res Commun (2011): 211

Visualizing dengue virus through Alexa Fluor labeling

Authors:

Zhang S, Tan HC, Ooi EE.

Journal:

J Vis Exp. (2011)

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes

Authors:

Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.

Journal:

Mol Biosyst (2011): 1766

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers

Authors:

Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.

Journal:

J Microbiol Methods (2011): 119

Physical properties

Molecular weight	716.57
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.21
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 23, 2026