iFluor® 488 azide

Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with iFluor® 488 dyes are far superior to conjugates of fluorescein derivatives such as FITC. iFluor® 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor® 488 is not affected by pH (4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. iFluor® 488 azide dye is reasonably stable and shows good reactivity and selectivity with the alkyne group for click chemistry applications. The iFluor® 488 dyes are an excellent replacement to the Alexa Fluor dyes that have identical spectral properties ( Alexa Fluor® is the trademark of Invitrogen).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 488 azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	139.554 µL	697.769 µL	1.396 mL	6.978 mL	13.955 mL
5 mM	27.911 µL	139.554 µL	279.107 µL	1.396 mL	2.791 mL
10 mM	13.955 µL	69.777 µL	139.554 µL	697.769 µL	1.396 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 405 azide	403	427	37000¹	0.91¹	0.48	0.77
iFluor®488-dUTP 1 mM in TE Buffer (pH 7.5)	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 Styramide Superior Replacement for Alexa Fluor 488 tyramide and Opal 520	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 TCO	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 Tetrazine	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 555 azide	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 594 azide	587	603	200000¹	0.53¹	0.05	0.04
iFluor® 647 azide	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 790 Azide	787	812	250000¹	0.13¹	0.1	0.09

Citations

View all 37 citations: Citation Explorer

Genetic modeling of ELP1-associated Sonic hedgehog medulloblastoma identifies MDM2 as a selective therapeutic target
Authors: Ahmad, Shiekh Tanveer and Li, Yiran and Garcia-Lopez, Jesus and Gudenas, Brian L and Hadley, Jennifer and Paul, Leena and Wu, Stephanie C and Refaat, Alaa and Kojic, Marija and Batts, Melissa and others,
Journal: Cancer Cell (2025)

CARM1 S217 phosphorylation by CDK1 in late G2 phase facilitates mitotic entry
Authors: Cho, Yena and Song, Dae-Geun and Kim, Su-Nam and Kim, Yong Kee
Journal: Cell Death \& Disease (2025): 202

CARM1 regulates tubulin autoregulation through PI3KC2$\alpha$ R175 methylation
Authors: Cho, Yena and Hwang, Jee Won and Bedford, Mark T and Song, Dae-Geun and Kim, Su-Nam and Kim, Yong Kee
Journal: Cell Communication and Signaling (2025): 120

Homoscleromorpha): A New Convenient Model for Sponge Cell and Evolutionary Developmental Biology
Authors: Rocher, Caroline and Vernale, Am{\'e}lie and Fierro-Consta{\'\i}n, Laura and S{\'e}journ{\'e}, Nina and Chenesseau, Sandrine and Marschal, Christian and Issartel, Julien and Le Goff, Emilie and Stroebel, David and Jouvion, Julie and others,
Journal: (2024)

Vibrational Markers of a Model Circulating Metastatic Cells LLC-R9
Authors: Gnatyuk, Olena and Kolesnyk, Denys and Voitsitskyi, Taras and Karakhim, Sergiy and Nikolenko, Andriy and Dementjev, Andrej and Solyanik, Galina and Dovbeshko, Galyna
Journal: Spectroscopy Journal (2024): 306--321

References

View all 49 references: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes
Authors: Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.
Journal: Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells
Authors: Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.
Journal: Biochem Biophys Res Commun (2011): 211

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
Authors: Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.
Journal: Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers
Authors: Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.
Journal: J Microbiol Methods (2011): 119

Page updated on May 19, 2025

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Catalog Number	1000
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Physical properties

Molecular weight	716.57
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.21
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501