iFluor® 488 azide
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 716.57 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.21 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 750001 |
Excitation (nm) | 491 |
Emission (nm) | 516 |
Quantum yield | 0.91 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
See also: Fluoresceins, iFluor® Dyes and Kits
Molecular weight 716.57 | Correction Factor (260 nm) 0.21 | Correction Factor (280 nm) 0.11 | Extinction coefficient (cm -1 M -1) 750001 | Excitation (nm) 491 | Emission (nm) 516 | Quantum yield 0.91 |
Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with iFluor® 488 dyes are far superior to conjugates of fluorescein derivatives such as FITC. iFluor® 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor® 488 is not affected by pH (4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. iFluor® 488 azide dye is reasonably stable and shows good reactivity and selectivity with the alkyne group for click chemistry applications. The iFluor® 488 dyes are an excellent replacement to the Alexa Fluor dyes that have identical spectral properties ( Alexa Fluor® is the trademark of Invitrogen).
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 488 azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 139.554 µL | 697.769 µL | 1.396 mL | 6.978 mL | 13.955 mL |
5 mM | 27.911 µL | 139.554 µL | 279.107 µL | 1.396 mL | 2.791 mL |
10 mM | 13.955 µL | 69.777 µL | 139.554 µL | 697.769 µL | 1.396 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Correction Factor (260 nm) | 0.21 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 750001 |
Excitation (nm) | 491 |
Emission (nm) | 516 |
Quantum yield | 0.91 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 647 azide | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
iFluor® 488 tyramide | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 555 azide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 488 Styramide *Superior Replacement for Alexa Fluor 488 tyramide and Opal 520* | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 488 TCO | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 488 Tetrazine | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor®488-dUTP *1 mM in Tris Buffer (pH 7.5)* | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor™ 405 azide | 403 | 427 | 370001 | 0.911 | 0.48 | 0.77 |
Images

Figure 1. Click chemistry is a method for attaching a probe or substrate of interest to a specific biomolecule, a process called bioconjugation. The possibility of attaching fluorophores and other reporter molecules has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an azide with an alkyne to form a 5-membered heteroatom ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC).
Citations
View all 22 citations: Citation Explorer
m6A-modified circNFIX promotes ovarian cancer progression and immune escape via activating IL-6R/JAK1/STAT3 signaling by sponging miR-647
Authors: Wang, Ruiyu and Ye, Hui and Yang, Bowen and Ao, Mengyin and Yu, Xiuzhang and Wu, Yuke and Xi, Mingrong and Hou, Minmin
Journal: International Immunopharmacology (2023): 110879
Authors: Wang, Ruiyu and Ye, Hui and Yang, Bowen and Ao, Mengyin and Yu, Xiuzhang and Wu, Yuke and Xi, Mingrong and Hou, Minmin
Journal: International Immunopharmacology (2023): 110879
Brassica napus Roots Use Different Strategies to Respond to Warm Temperatures
Authors: Boter, Marta and Pozas, Jenifer and Jarillo, Jose A and Pi{\~n}eiro, Manuel and Pernas, M{\'o}nica
Journal: International Journal of Molecular Sciences (2023): 1143
Authors: Boter, Marta and Pozas, Jenifer and Jarillo, Jose A and Pi{\~n}eiro, Manuel and Pernas, M{\'o}nica
Journal: International Journal of Molecular Sciences (2023): 1143
Vpx requires active cellular dNTP biosynthesis to effectively counteract the anti-lentivirus activity of SAMHD1 in macrophages.
Authors: Bowen, Nicole E and Tao, Sijia and Cho, Young-Jae and Schinazi, Raymond F and Kim, Baek
Journal: Journal of Biological Chemistry (2023): 104984
Authors: Bowen, Nicole E and Tao, Sijia and Cho, Young-Jae and Schinazi, Raymond F and Kim, Baek
Journal: Journal of Biological Chemistry (2023): 104984
Functional Differentiation of Cyclins and Cyclin-Dependent Kinases in Giardia lamblia
Authors: Kim, Juri and Park, Eun-Ah and Shin, Mee Young and Park, Soon-Jung
Journal: Microbiology Spectrum (2023): e04919--22
Authors: Kim, Juri and Park, Eun-Ah and Shin, Mee Young and Park, Soon-Jung
Journal: Microbiology Spectrum (2023): e04919--22
Kukoamine A attenuates lipopolysaccharide-induced apoptosis, extracellular matrix degradation, and inflammation in nucleus pulposus cells by activating the P13K/Akt pathway
Authors: Wang, Dan and Qu, Hao and Kang, Hui and Xu, Feng and Huang, Wei and Cai, Xianhua
Journal: Bioengineered (2022): 8772--8784
Authors: Wang, Dan and Qu, Hao and Kang, Hui and Xu, Feng and Huang, Wei and Cai, Xianhua
Journal: Bioengineered (2022): 8772--8784
Fibromodulin and Its Peptide-based Therapy for Cutaneous and Diabetic Wound Healing
Authors: Chen, Yao
Journal: (2022)
Authors: Chen, Yao
Journal: (2022)
Independent phenotypic plasticity axes define distinct obesity sub-types
Authors: Yang, Chih-Hsiang and Fagnocchi, Luca and Apostle, Stefanos and Wegert, Vanessa and Casan{\'\i}-Gald{\'o}n, Salvador and Landgraf, Kathrin and Panzeri, Ilaria and Dror, Erez and Heyne, Steffen and W{\"o}rpel, Till and others,
Journal: Nature metabolism (2022): 1150--1165
Authors: Yang, Chih-Hsiang and Fagnocchi, Luca and Apostle, Stefanos and Wegert, Vanessa and Casan{\'\i}-Gald{\'o}n, Salvador and Landgraf, Kathrin and Panzeri, Ilaria and Dror, Erez and Heyne, Steffen and W{\"o}rpel, Till and others,
Journal: Nature metabolism (2022): 1150--1165
ZBTB7A promotes virus-host homeostasis during human coronavirus 229E infection
Authors: Zhu, Xinyu and Trimarco, Joseph D and Williams, Courtney A and Barrera, Alejandro and Reddy, Timothy E and Heaton, Nicholas S
Journal: Cell Reports (2022): 111540
Authors: Zhu, Xinyu and Trimarco, Joseph D and Williams, Courtney A and Barrera, Alejandro and Reddy, Timothy E and Heaton, Nicholas S
Journal: Cell Reports (2022): 111540
Osteoclasts directly influence castration-resistant prostate cancer cells
Authors: Huang, Junchi and Freyhult, Eva and Buckland, Robert and Josefsson, Andreas and Damber, Jan-Erik and Wel{\'e}n, Karin
Journal: Clinical \& experimental metastasis (2022): 801--814
Authors: Huang, Junchi and Freyhult, Eva and Buckland, Robert and Josefsson, Andreas and Damber, Jan-Erik and Wel{\'e}n, Karin
Journal: Clinical \& experimental metastasis (2022): 801--814
A Novel CDK4/6 and PARP Dual Inhibitor ZC-22 Effectively Suppresses Tumor Growth and Improves the Response to Cisplatin Treatment in Breast and Ovarian Cancer
Authors: Tian, Chenchen and Wei, Yufan and Li, Jianjun and Huang, Zhi and Wang, Qiong and Lin, Yingxue and Lv, Xingping and Chen, Yanan and Fan, Yan and Sun, Peiqing and others,
Journal: International journal of molecular sciences (2022): 2892
Authors: Tian, Chenchen and Wei, Yufan and Li, Jianjun and Huang, Zhi and Wang, Qiong and Lin, Yingxue and Lv, Xingping and Chen, Yanan and Fan, Yan and Sun, Peiqing and others,
Journal: International journal of molecular sciences (2022): 2892
References
View all 49 references: Citation Explorer
Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes
Authors: Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.
Journal: Mol Biosyst (2011): 1766
Authors: Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.
Journal: Mol Biosyst (2011): 1766
Visualizing dengue virus through Alexa Fluor labeling
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)
Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells
Authors: Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.
Journal: Biochem Biophys Res Commun (2011): 211
Authors: Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.
Journal: Biochem Biophys Res Commun (2011): 211
Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
Authors: Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.
Journal: Zhen Ci Yan Jiu (2011): 262
Authors: Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.
Journal: Zhen Ci Yan Jiu (2011): 262
Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers
Authors: Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.
Journal: J Microbiol Methods (2011): 119
Authors: Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.
Journal: J Microbiol Methods (2011): 119
Alexa Fluor 546-ArIB[V11L;V16A] is a potent ligand for selectively labeling alpha 7 nicotinic acetylcholine receptors
Authors: Hone AJ, Whiteaker P, Mohn JL, Jacob MH, McIntosh JM.
Journal: J Neurochem (2010): 994
Authors: Hone AJ, Whiteaker P, Mohn JL, Jacob MH, McIntosh JM.
Journal: J Neurochem (2010): 994
Asymmetric trimethine 3H-indocyanine dyes: efficient synthesis and protein labeling
Authors: Song F, Wang L, Qiao X, Wang B, Sun S, Fan J, Zhang L, Peng X.
Journal: Org Biomol Chem (2010): 4249
Authors: Song F, Wang L, Qiao X, Wang B, Sun S, Fan J, Zhang L, Peng X.
Journal: Org Biomol Chem (2010): 4249
Neuroanatomical characteristics of acupoint "Chengshan" (BL 57) in the rat: a cholera toxin subunit B conjugated with Alexa Fluor 488 method study
Authors: Zhu XL, Bai WZ, Wu FD, Jiang J, Jing XH.
Journal: Zhen Ci Yan Jiu (2010): 433
Authors: Zhu XL, Bai WZ, Wu FD, Jiang J, Jing XH.
Journal: Zhen Ci Yan Jiu (2010): 433
Photoactivatable and photoconvertible fluorescent probes for protein labeling
Authors: Maurel D, Banala S, Laroche T, Johnsson K.
Journal: ACS Chem Biol (2010): 507
Authors: Maurel D, Banala S, Laroche T, Johnsson K.
Journal: ACS Chem Biol (2010): 507
Novel Alexa Fluor-488 labeled antagonist of the A(2A) adenosine receptor: Application to a fluorescence polarization-based receptor binding assay
Authors: Kecskes M, Kumar TS, Yoo L, Gao ZG, Jacobson KA.
Journal: Biochem Pharmacol (2010): 506
Authors: Kecskes M, Kumar TS, Yoo L, Gao ZG, Jacobson KA.
Journal: Biochem Pharmacol (2010): 506
Application notes
iFluor® Dye Selection Guide
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
FAQ
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?