iFluor® 488 amine

iFluor® 488 Amine is a green-fluorescent dye with a primary amine group for covalent modification of carbonyl-containing compounds in fluorescence-based research applications.

Amine Functional Group: Enables conjugation to aldehydes and activated carboxyl groups.
Spectral Properties: Excitation at 491 nm and emission at 516 nm.
Fluorescence Performance: Bright, photostable fluorescence with a quantum yield of 0.90.
pH Sensitivity: Maintains stable fluorescence across pH 4–10.
Applications: Suitable for fluorescent modification of carbonyl-containing molecules and biomolecular conjugates.

<p>Fluorescent dye amines are the most popular tool for conjugating dyes to a target compound with a carbonyl group (e.g., aldehyde, carboxylic acid or activated carboxy group such as NHS ester).</p>

Datasheet

SDS

Catalog	Size	Price	Quantity
1072	1 mg	Price

Overview

Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with iFluor® 488 dyes are far superior to conjugates of fluorescein derivatives such as FITC. iFluor® 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor® 488 is not affected by pH (4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. iFluor® 488 amine is stable and used for modifying carbonyl groups (e.g., aldehyde and carboxy groups).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 488 amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	138.922 µL	694.608 µL	1.389 mL	6.946 mL	13.892 mL
5 mM	27.784 µL	138.922 µL	277.843 µL	1.389 mL	2.778 mL
10 mM	13.892 µL	69.461 µL	138.922 µL	694.608 µL	1.389 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	719.83 g/mol	=	mL	x	mM	=	mmol

Citations

View all 32 citations: Citation Explorer

The teneurin TEN-1 and its ligand LRON-11 support microbiota-induced neuroprotection and mechanosensory function in C. elegans

Authors:

Li, Jingxian and Castillo, Juan Pablo and Cheng, Shouqiang and Weathers, Indya and Nawrocka, Wioletta I and Alvarado, Jorge and Zhao, Minglei and Calixto, Andrea and {\"O}zkan, Engin and Ara{\c{c}}, Demet

Journal:

Current Biology (2026)

A Signal Peptide Screening Approach for Improved Fc-Fusion Expression in CHO-S Cells

Authors:

Holm, Isak

Fully Human Antagonistic Antibodies Targeting FPR2 Through Dual Extracellular-Loop Engagement for Gastric Cancer Therapy

Authors:

Kim, Min Su and Kang, Tae Hyun

Journal:

Antibody Therapeutics (2026): tbag035

Analysis of paired primary-metastatic renal cancer lesions reveals RCN3-MMP10 axis facilitates lung metastasis

Authors:

Lin, Wenhao and Wang, Qi and Xu, Kandi and Ding, Jiawei and Ma, Zhiyang and Xing, Siwei and Xu, Danfeng and He, Yayi and Chen, Lu

Journal:

Cellular Signalling (2026): 112534

Cationic mRNA Lipid Nanoparticles for Ex Vivo NanoCAR-T Cell Engineering

Authors:

Harinck, Laure and De Munter, Stijn and De Velder, Margo and Ingels, Joline and Berdecka, Dominika and Lentacker, Ine and De Vos, Winnok H and Vandekerckhove, Bart and Braeckmans, Kevin and Raemdonck, Koen

Journal:

Advanced Science (2026): e21507

References

View all 49 references: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes

Authors:

Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.

Journal:

Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling

Authors:

Zhang S, Tan HC, Ooi EE.

Journal:

J Vis Exp. (2011)

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells

Authors:

Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.

Journal:

Biochem Biophys Res Commun (2011): 211

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Authors:

Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.

Journal:

Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers

Authors:

Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.

Journal:

J Microbiol Methods (2011): 119

Physical properties

Molecular weight	719.83
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.21
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 28, 2026