Name: iFluor® 647 Styramide™
Brand: AAT Bioquest
SKU: 45045
Price: 355 USD
Availability: InStock

iFluor® 647 Styramide™

iFluor® 647 Styramide™ is a far-red fluorescent signal amplification reagent designed for sensitive detection of low-abundance targets in cells and tissues using HRP-mediated labeling.

Detection Principle: Uses HRP-catalyzed covalent deposition of fluorescent labels.
High Sensitivity: Generates 10–50 times stronger fluorescence signals than conventional tyramide reagents.
Spectral Properties: Excitation/emission maxima of 655/670 nm.
Filter Compatibility: Compatible with Cy5 fluorescence microscope filter sets.
Applications: Suitable for immunocytochemistry, immunofluorescence, and immunohistochemistry.

Fluorescence IHC of formaldehyde-fixed, paraffin-embedded human lung adenocarcinoma positive tissue using PSA™ and TSA amplified methods. Human lung adenocarcinoma positive tissue sections were stained with rabbit anti-EpCaM antibody and then incubated with poly HRP-labeled Goat anti-Rabbit IgG secondary antibody followed by iFluor® 647 Styramide™ (Cat#45045) or Alexa Fluor® 647 tyramide stain, respectively. Images showed that iFluor® 647 PSA™ super signal amplification can increase the sensitivity of fluorescence IHC over Alexa Fluor® 647 TSA method.

Protocol

SDS

Catalog	Size	Price	Quantity
45045	100 Slides	Price

Overview

Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA™ utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA™ radicals have much higher reactivity than tyramide radicals, making the PSA™ system much faster, more robust and sensitive than the traditional TSA reagents. Compared to tyramide reagents, the Styramide™ conjugates have ability to label the target at higher efficiency and thus generate significantly higher fluorescence signal. Styramide™ conjugates also allow significantly less consumption of primary antibody compared to standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 647 Styramide is a superior replacement for Alexa Fluor 647 tyramide or other spectrally similar fluorescent tyramide conjugates or TSA reagents.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 647 Styramide™ to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	81.193 µL	405.966 µL	811.932 µL	4.06 mL	8.119 mL
5 mM	16.239 µL	81.193 µL	162.386 µL	811.932 µL	1.624 mL
10 mM	8.119 µL	40.597 µL	81.193 µL	405.966 µL	811.932 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1231.63 g/mol	=	mL	x	mM	=	mmol

Citations

View all 9 citations: Citation Explorer

Tyramide signal amplification-based detection system: A novel approach to improve detection efficiency for circulating tumor cells

Authors:

Chen, Lin and Yang, Zhonglin and Lu, Yue and Li, Shan and Tang, Dongjiang and Zhang, Lei

Journal:

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy (2025): 126898

Membrane progesterone receptor $\gamma$ (paqr5b) is essential for the formation of neurons in the zebrafish olfactory rosette

Authors:

Mustary, Umme Habiba and Maeno, Akiteru and Rahaman, Md Mostafizur and Ali, Md Hasan and Tokumoto, Toshinobu

Journal:

Scientific Reports (2024): 24354

The tandem CD33-CLL1 CAR-T as an approach to treat acute myeloid leukemia: The tandem CLL1/CD33 CAR-T to treat AML

Authors:

Wang, Huiru and Feng, Shanglong and Zhu, Yanliang and Zhang, Yafeng and Zhou, Ziwei and Nian, Zhigang and Lu, Xueqin and Peng, Peng and Wu, Shu and Zhou, Li

Journal:

Blood Transfusion (2024)

Site-specific labeling and functional efficiencies of human fibroblast growth Factor-1 with a range of fluorescent Dyes in the flexible N-Terminal region and a rigid $\beta$-turn region

Authors:

Mohale, Mamello and Gundampati, Ravi Kumar and Kumar, Thallapuranam Krishnaswamy Suresh and Heyes, Colin D

Journal:

Analytical biochemistry (2022): 114524

SP/NK-1R Axis Promotes Perineural Invasion of Pancreatic Cancer and is Affected by lncRNA LOC389641

Authors:

Ji, Tengfei and Ma, Keqiang and Wu, Hongsheng and Cao, Tiansheng

Physical properties

Molecular weight	1231.63
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.03
Correction factor (280 nm)	0.03
Correction factor (656 nm)	0.0793
Extinction coefficient (cm ^-1 M ^-1)	220000 ¹
Excitation (nm)	655
Emission (nm)	670
Quantum yield	0.25 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Fluorescence microscope
Excitation	Cy5 filter set
Emission	Cy5 filter set
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 30, 2026