iFluor® 647 TCO

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	1181.33
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.03
Correction Factor (280 nm)	0.03
Correction Factor (656 nm)	0.0793
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	656
Emission (nm)	670
Quantum yield	0.25¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 647 TCO to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	84.65 µL	423.252 µL	846.504 µL	4.233 mL	8.465 mL
5 mM	16.93 µL	84.65 µL	169.301 µL	846.504 µL	1.693 mL
10 mM	8.465 µL	42.325 µL	84.65 µL	423.252 µL	846.504 µL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.03
Correction Factor (280 nm)	0.03
Correction Factor (656 nm)	0.0793
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	656
Emission (nm)	670
Quantum yield	0.25¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)	Correction Factor (656 nm)
iFluor® 647 maleimide	656	670	250000¹	0.25¹	0.03	0.03	0.0793
iFluor® 647 amine	656	670	250000¹	0.25¹	0.03	0.03	0.0793
iFluor® 647 hydrazide	656	670	250000¹	0.25¹	0.03	0.03	0.0793
iFluor® 647 alkyne	656	670	250000¹	0.25¹	0.03	0.03	0.0793
iFluor® 647 azide	656	670	250000¹	0.25¹	0.03	0.03	0.0793
iFluor® 647 Styramide Superior Replacement for Alexa Fluor 647 tyramide	656	670	250000¹	0.25¹	0.03	0.03	0.0793
iFluor® 647 Tyramide	656	670	250000¹	0.25¹	0.03	0.03	0.0793
iFluor® 488 TCO	491	516	75000¹	0.9¹	0.21	0.11	-
iFluor® 555 TCO	557	570	100000¹	0.64¹	0.23	0.14	-
iFluor® 594 TCO	587	603	200000¹	0.53¹	0.05	0.04	-
iFluor® 647 Tetrazine	656	670	250000¹	0.25¹	0.03	0.03	0.0793
Show More (2)

Images

Figure 1. The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. iFluor® 647-TCO can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis.

Citations

View all 7 citations: Citation Explorer

Immune-regulating bimetallic metal-organic framework nanoparticles designed for cancer immunotherapy
Authors: Dai, Zan and Wang, Qiaoyun and Tang, Jie and Wu, Min and Li, Haoze and Yang, Yannan and Zhen, Xu and Yu, Chengzhong
Journal: Biomaterials (2022): 121261

Site-specific labeling and functional efficiencies of human fibroblast growth Factor-1 with a range of fluorescent Dyes in the flexible N-Terminal region and a rigid $\beta$-turn region
Authors: Mohale, Mamello and Gundampati, Ravi Kumar and Kumar, Thallapuranam Krishnaswamy Suresh and Heyes, Colin D
Journal: Analytical biochemistry (2022): 114524

SP/NK-1R Axis Promotes Perineural Invasion of Pancreatic Cancer and is Affected by lncRNA LOC389641
Authors: Ji, Tengfei and Ma, Keqiang and Wu, Hongsheng and Cao, Tiansheng
Journal: (2021)

Efferocytosis induces macrophage proliferation to help resolve tissue injury
Authors: Gerlach, Brennan D and Ampomah, Patrick B and Yurdagul Jr, Arif and Liu, Chuang and Lauring, Max C and Wang, Xiaobo and Kasikara, Canan and Kong, Na and Shi, Jinjun and Tao, Wei and others,
Journal: Cell metabolism (2021): 2445--2463

Enrichment of NPC1-deficient cells with the lipid LBPA stimulates autophagy, improves lysosomal function, and reduces cholesterol storage
Authors: Ilnytska, Olga and Lai, Kimberly and Gorshkov, Kirill and Schultz, Mark L and Tran, Bruce Nguyen and Jeziorek, Maciej and Kunkel, Thaddeus J and Azaria, Ruth D and McLoughlin, Hayley S and Waghalter, Miriam and others,
Journal: Journal of Biological Chemistry (2021)

Pharmacological targeting of Sam68 functions in colorectal cancer stem cells
Authors: Masibag, Angelique N and Bergin, Christopher J and Haebe, Joshua R and Zouggar, A{\"\i}cha and Shah, Muhammad S and Sandouka, Tamara and da Silva, Amanda Mendes and Desrochers, Fran{\c{c}}ois M and Fournier-Morin, Aube and Benoit, Yannick D
Journal: Iscience (2021): 103442

Influence of particle geometry on gastrointestinal transit and absorption following oral administration
Authors: Li, Dong and Zhuang, Jie and He, Haisheng and Jiang, Sifan and Banerjee, Amrita and Lu, Yi and Wu, Wei and Mitragotri, Samir and Gan, Li and Qi, Jianping
Journal: ACS applied materials \& interfaces (2017): 42492--42502

References

View all 50 references: Citation Explorer

Bond-Breaking Bio-orthogonal Chemistry Efficiently Uncages Fluorescent and Therapeutic Compounds under Physiological Conditions.
Authors: Wu, Xunshen and Wu, Kui and Gaye, Fatima and Royzen, Maksim
Journal: Organic letters (2020): 6041-6044

Recent advances in bio-orthogonal and dynamic crosslinking of biomimetic hydrogels.
Authors: Arkenberg, Matthew R and Nguyen, Han D and Lin, Chien-Chi
Journal: Journal of materials chemistry. B (2020)

Profiling the Protein Targets of Unmodified Bio-Active Molecules with Drug Affinity Responsive Target Stability and Liquid Chromatography/Tandem Mass Spectrometry.
Authors: Hwang, Hui-Yun and Kim, Tae Young and Szász, Marcell A and Dome, Balazs and Malm, Johan and Marko-Varga, Gyorgy and Kwon, Ho Jeong
Journal: Proteomics (2020): e1900325

Bio-orthogonal click-targeting nanocomposites for chemo-photothermal synergistic therapy in breast cancer.
Authors: Qiao, Jianan and Tian, Fengchun and Deng, Yudi and Shang, Yunkai and Chen, Shijie and Chang, Enhao and Yao, Jing
Journal: Theranostics (2020): 5305-5321

Click-to-Capture: A method for enriching viable Staphylococcus aureus using bio-orthogonal labeling of surface proteins.
Authors: Shalizi, Aryaman and Wiegers, Toni N and Maamar, Hédia
Journal: PloS one (2020): e0234542

Engineering the Bio-Nano Interface Using a Multifunctional Coordinating Polymer Coating.
Authors: Wang, Wentao and Mattoussi, Hedi
Journal: Accounts of chemical research (2020): 1124-1138

A bio-responsive 6-mercaptopurine/doxorubicin based "Click Chemistry" polymeric prodrug for cancer therapy.
Authors: Liao, Jianhong and Peng, Haisheng and Wei, Xuan and Song, Yajing and Liu, Can and Li, Dan and Yin, Yihua and Xiong, Xiong and Zheng, Hua and Wang, Qun
Journal: Materials science & engineering. C, Materials for biological applications (2020): 110461

Titanium coating with mussel inspired polymer and bio-orthogonal chemistry enhances antimicrobial activity against Staphylococcus aureus.
Authors: Czuban, Magdalena and Kulka, Michaël W and Wang, Lei and Koliszak, Anna and Achazi, Katharina and Schlaich, Christoph and Donskyi, Ievgen S and Di Luca, Mariagrazia and Mejia Oneto, Jose M and Royzen, Maksim and Haag, Rainer and Trampuz, Andrej
Journal: Materials science & engineering. C, Materials for biological applications (2020): 111109

Global analysis of RNA metabolism using bio-orthogonal labeling coupled with next-generation RNA sequencing.
Authors: Wolfe, Michael B and Goldstrohm, Aaron C and Freddolino, Peter L
Journal: Methods (San Diego, Calif.) (2019): 88-103

Facile Synthesis of a 3,4-Ethylene-Dioxythiophene (EDOT) Derivative for Ease of Bio-Functionalization of the Conducting Polymer PEDOT.
Authors: Wu, Bingchen and Cao, Bin and Taylor, Ian Mitch and Woeppel, Kevin and Cui, Xinyan Tracy
Journal: Frontiers in chemistry (2019): 178