iFluor® 647 TCO
The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction. iFluor® 647-TCO can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biological applications. The conjugates prepared with iFluor® 647 dye have spectral properties almost identical to the popular Cy5 and Alexa Fluor® 647. In most cases, antibody conjugates prepared with iFluor® 647 tend to have a higher signal/background ratio than the spectrally similar dye conjugates, such as Cy5 and Alexa Fluor® 647 (Alexa Fluor® is the trademark of Invitrogen).
Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) | Correction Factor (656 nm) |
iFluor® 647 maleimide | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 | 0.0793 |
iFluor® 647 amine | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 | 0.0793 |
iFluor® 647 hydrazide | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 | 0.0793 |
iFluor® 647 alkyne | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 | 0.0793 |
iFluor® 647 azide | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 | 0.0793 |
iFluor® 647 Styramide *Superior Replacement for Alexa Fluor 647 tyramide* | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 | 0.0793 |
iFluor® 647 Tyramide | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 | 0.0793 |
iFluor® 488 TCO | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 | - |
iFluor® 555 TCO | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 | - |
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Citations
View all 8 citations: Citation Explorer
The tandem CD33-CLL1 CAR-T as an approach to treat acute myeloid leukemia: The tandem CLL1/CD33 CAR-T to treat AML
Authors: Wang, Huiru and Feng, Shanglong and Zhu, Yanliang and Zhang, Yafeng and Zhou, Ziwei and Nian, Zhigang and Lu, Xueqin and Peng, Peng and Wu, Shu and Zhou, Li
Journal: Blood Transfusion (2024)
Authors: Wang, Huiru and Feng, Shanglong and Zhu, Yanliang and Zhang, Yafeng and Zhou, Ziwei and Nian, Zhigang and Lu, Xueqin and Peng, Peng and Wu, Shu and Zhou, Li
Journal: Blood Transfusion (2024)
Immune-regulating bimetallic metal-organic framework nanoparticles designed for cancer immunotherapy
Authors: Dai, Zan and Wang, Qiaoyun and Tang, Jie and Wu, Min and Li, Haoze and Yang, Yannan and Zhen, Xu and Yu, Chengzhong
Journal: Biomaterials (2022): 121261
Authors: Dai, Zan and Wang, Qiaoyun and Tang, Jie and Wu, Min and Li, Haoze and Yang, Yannan and Zhen, Xu and Yu, Chengzhong
Journal: Biomaterials (2022): 121261
Site-specific labeling and functional efficiencies of human fibroblast growth Factor-1 with a range of fluorescent Dyes in the flexible N-Terminal region and a rigid $\beta$-turn region
Authors: Mohale, Mamello and Gundampati, Ravi Kumar and Kumar, Thallapuranam Krishnaswamy Suresh and Heyes, Colin D
Journal: Analytical biochemistry (2022): 114524
Authors: Mohale, Mamello and Gundampati, Ravi Kumar and Kumar, Thallapuranam Krishnaswamy Suresh and Heyes, Colin D
Journal: Analytical biochemistry (2022): 114524
SP/NK-1R Axis Promotes Perineural Invasion of Pancreatic Cancer and is Affected by lncRNA LOC389641
Authors: Ji, Tengfei and Ma, Keqiang and Wu, Hongsheng and Cao, Tiansheng
Journal: (2021)
Authors: Ji, Tengfei and Ma, Keqiang and Wu, Hongsheng and Cao, Tiansheng
Journal: (2021)
Pharmacological targeting of Sam68 functions in colorectal cancer stem cells
Authors: Masibag, Angelique N and Bergin, Christopher J and Haebe, Joshua R and Zouggar, A{\"\i}cha and Shah, Muhammad S and Sandouka, Tamara and da Silva, Amanda Mendes and Desrochers, Fran{\c{c}}ois M and Fournier-Morin, Aube and Benoit, Yannick D
Journal: Iscience (2021): 103442
Authors: Masibag, Angelique N and Bergin, Christopher J and Haebe, Joshua R and Zouggar, A{\"\i}cha and Shah, Muhammad S and Sandouka, Tamara and da Silva, Amanda Mendes and Desrochers, Fran{\c{c}}ois M and Fournier-Morin, Aube and Benoit, Yannick D
Journal: Iscience (2021): 103442
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