iFluor® 647 TCO

The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction. iFluor® 647-TCO can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biological applications. The conjugates prepared with iFluor® 647 dye have spectral properties almost identical to the popular Cy5 and Alexa Fluor® 647. In most cases, antibody conjugates prepared with iFluor® 647 tend to have a higher signal/background ratio than the spectrally similar dye conjugates, such as Cy5 and Alexa Fluor® 647 (Alexa Fluor® is the trademark of Invitrogen).

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)	Correction Factor (656 nm)
iFluor® 488 TCO	491	516	75000¹	0.9¹	0.21	0.11	-
iFluor® 555 TCO	557	570	100000¹	0.64¹	0.23	0.14	-
iFluor® 594 TCO	587	603	200000¹	0.53¹	0.05	0.04	-
iFluor® 647 acid	656	670	250000¹	0.25¹	0.03	0.03	0.0793
iFluor® 647 alkyne	656	670	250000¹	0.25¹	0.03	0.03	0.0793
iFluor® 647 amine	656	670	250000¹	0.25¹	0.03	0.03	0.0793
iFluor® 647 azide	656	670	250000¹	0.25¹	0.03	0.03	0.0793
iFluor® 647 hydrazide	656	670	250000¹	0.25¹	0.03	0.03	0.0793
iFluor® 647 maleimide	656	670	250000¹	0.25¹	0.03	0.03	0.0793
iFluor® 647 Styramide Superior Replacement for Alexa Fluor 647 tyramide	656	670	250000¹	0.25¹	0.03	0.03	0.0793
iFluor® 647 Tetrazine	656	670	250000¹	0.25¹	0.03	0.03	0.0793
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Citations

View all 8 citations: Citation Explorer

The tandem CD33-CLL1 CAR-T as an approach to treat acute myeloid leukemia: The tandem CLL1/CD33 CAR-T to treat AML
Authors: Wang, Huiru and Feng, Shanglong and Zhu, Yanliang and Zhang, Yafeng and Zhou, Ziwei and Nian, Zhigang and Lu, Xueqin and Peng, Peng and Wu, Shu and Zhou, Li
Journal: Blood Transfusion (2024)

Immune-regulating bimetallic metal-organic framework nanoparticles designed for cancer immunotherapy
Authors: Dai, Zan and Wang, Qiaoyun and Tang, Jie and Wu, Min and Li, Haoze and Yang, Yannan and Zhen, Xu and Yu, Chengzhong
Journal: Biomaterials (2022): 121261

Site-specific labeling and functional efficiencies of human fibroblast growth Factor-1 with a range of fluorescent Dyes in the flexible N-Terminal region and a rigid $\beta$-turn region
Authors: Mohale, Mamello and Gundampati, Ravi Kumar and Kumar, Thallapuranam Krishnaswamy Suresh and Heyes, Colin D
Journal: Analytical biochemistry (2022): 114524

SP/NK-1R Axis Promotes Perineural Invasion of Pancreatic Cancer and is Affected by lncRNA LOC389641
Authors: Ji, Tengfei and Ma, Keqiang and Wu, Hongsheng and Cao, Tiansheng
Journal: (2021)

Pharmacological targeting of Sam68 functions in colorectal cancer stem cells
Authors: Masibag, Angelique N and Bergin, Christopher J and Haebe, Joshua R and Zouggar, A{\"\i}cha and Shah, Muhammad S and Sandouka, Tamara and da Silva, Amanda Mendes and Desrochers, Fran{\c{c}}ois M and Fournier-Morin, Aube and Benoit, Yannick D
Journal: Iscience (2021): 103442

References

View all 50 references: Citation Explorer

A bio-responsive 6-mercaptopurine/doxorubicin based "Click Chemistry" polymeric prodrug for cancer therapy.
Authors: Liao, Jianhong and Peng, Haisheng and Wei, Xuan and Song, Yajing and Liu, Can and Li, Dan and Yin, Yihua and Xiong, Xiong and Zheng, Hua and Wang, Qun
Journal: Materials science & engineering. C, Materials for biological applications (2020): 110461

Titanium coating with mussel inspired polymer and bio-orthogonal chemistry enhances antimicrobial activity against Staphylococcus aureus.
Authors: Czuban, Magdalena and Kulka, Michaël W and Wang, Lei and Koliszak, Anna and Achazi, Katharina and Schlaich, Christoph and Donskyi, Ievgen S and Di Luca, Mariagrazia and Mejia Oneto, Jose M and Royzen, Maksim and Haag, Rainer and Trampuz, Andrej
Journal: Materials science & engineering. C, Materials for biological applications (2020): 111109

Engineering the Bio-Nano Interface Using a Multifunctional Coordinating Polymer Coating.
Authors: Wang, Wentao and Mattoussi, Hedi
Journal: Accounts of chemical research (2020): 1124-1138

Click-to-Capture: A method for enriching viable Staphylococcus aureus using bio-orthogonal labeling of surface proteins.
Authors: Shalizi, Aryaman and Wiegers, Toni N and Maamar, Hédia
Journal: PloS one (2020): e0234542

Profiling the Protein Targets of Unmodified Bio-Active Molecules with Drug Affinity Responsive Target Stability and Liquid Chromatography/Tandem Mass Spectrometry.
Authors: Hwang, Hui-Yun and Kim, Tae Young and Szász, Marcell A and Dome, Balazs and Malm, Johan and Marko-Varga, Gyorgy and Kwon, Ho Jeong
Journal: Proteomics (2020): e1900325

Page updated on May 19, 2025

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Catalog Number	1011
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Physical properties

Molecular weight	1181.33
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.03
Correction Factor (280 nm)	0.03
Correction Factor (656 nm)	0.0793
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	656
Emission (nm)	670
Quantum yield	0.25¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501