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iFluor® 555 TCO
The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction. iFluor® 555-TCO can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biological applications. The peptide and oligo conjugates prepared with iFluor® 555 dye are far superior to the conjugates of other spectrally similar dyes, such as the popular Cy3 and Alexa Fluor® 555. iFluor® 555 conjugates are significantly brighter and much more photostable than the corresponding conjugates of Cy3 and Alexa Fluor® 555 (Alexa Fluor® is the trademark of Invitrogen).
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| iFluor® 488 TCO | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
| iFluor® 555 alkyne | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| iFluor® 555 amine | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| iFluor® 555 azide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| iFluor® 555 hydrazide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| iFluor® 555 maleimide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| iFluor® 555 Styramide *Superior Replacement for Alexa Fluor 555 tyramide and Opal 570* | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| iFluor® 555 Tetrazine | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| iFluor® 594 TCO | 587 | 603 | 2000001 | 0.531 | 0.05 | 0.04 |
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References
View all 50 references: Citation Explorer
Novel Fluorescent Labeled Octasilsesquioxanes Nanohybrids as Potential Materials for Latent Fingerprinting Detection.
Authors: O Dare, Enock and Vendrell-Criado, Victoria and Jiménez, M Consuelo and Perez-Ruiz, Raúl and Díaz Díaz, David
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020)
Authors: O Dare, Enock and Vendrell-Criado, Victoria and Jiménez, M Consuelo and Perez-Ruiz, Raúl and Díaz Díaz, David
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020)
Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes.
Authors: Stone, M Rhia L and Phetsang, Wanida and Cooper, Matthew A and Blaskovich, Mark A T
Journal: Journal of visualized experiments : JoVE (2020)
Authors: Stone, M Rhia L and Phetsang, Wanida and Cooper, Matthew A and Blaskovich, Mark A T
Journal: Journal of visualized experiments : JoVE (2020)
Target verification of artesunate-related antiviral drugs: Assessing the role of mitochondrial and regulatory proteins by click chemistry and fluorescence labeling.
Authors: Hahn, Friedrich and Niesar, Aischa and Wangen, Christina and Wild, Markus and Grau, Benedikt and Herrmann, Lars and Capci, Aysun and Adrait, Annie and Couté, Yohann and Tsogoeva, Svetlana B and Marschall, Manfred
Journal: Antiviral research (2020): 104861
Authors: Hahn, Friedrich and Niesar, Aischa and Wangen, Christina and Wild, Markus and Grau, Benedikt and Herrmann, Lars and Capci, Aysun and Adrait, Annie and Couté, Yohann and Tsogoeva, Svetlana B and Marschall, Manfred
Journal: Antiviral research (2020): 104861
A novel polythioether-based rhodamine B fluorescent probe via successive click reaction and its application in iron ion detection and cell imaging.
Authors: Zhang, Xiaomei and Gou, Zhiming and Zuo, Yujing and Lin, Weiying
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2020): 117679
Authors: Zhang, Xiaomei and Gou, Zhiming and Zuo, Yujing and Lin, Weiying
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2020): 117679
A glutathione-responsive photosensitizer with fluorescence resonance energy transfer characteristics for imaging-guided targeting photodynamic therapy.
Authors: Cao, Jing-Jing and Zhang, Ming-Shan and Li, Xiao-Qiang and Yang, De-Chao and Xu, Gan and Liu, Jian-Yong
Journal: European journal of medicinal chemistry (2020): 112203
Authors: Cao, Jing-Jing and Zhang, Ming-Shan and Li, Xiao-Qiang and Yang, De-Chao and Xu, Gan and Liu, Jian-Yong
Journal: European journal of medicinal chemistry (2020): 112203
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