iFluor® 555 TCO

The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction. iFluor® 555-TCO can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biological applications. The peptide and oligo conjugates prepared with iFluor® 555 dye are far superior to the conjugates of other spectrally similar dyes, such as the popular Cy3 and Alexa Fluor® 555. iFluor® 555 conjugates are significantly brighter and much more photostable than the corresponding conjugates of Cy3 and Alexa Fluor® 555 (Alexa Fluor® is the trademark of Invitrogen).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
1007	1 mg	Price

References

View all 50 references: Citation Explorer

Novel Fluorescent Labeled Octasilsesquioxanes Nanohybrids as Potential Materials for Latent Fingerprinting Detection.

Authors:

O Dare, Enock and Vendrell-Criado, Victoria and Jiménez, M Consuelo and Perez-Ruiz, Raúl and Díaz Díaz, David

Journal:

Chemistry (Weinheim an der Bergstrasse, Germany) (2020)

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes.

Authors:

Stone, M Rhia L and Phetsang, Wanida and Cooper, Matthew A and Blaskovich, Mark A T

Journal:

Journal of visualized experiments : JoVE (2020)

Target verification of artesunate-related antiviral drugs: Assessing the role of mitochondrial and regulatory proteins by click chemistry and fluorescence labeling.

Authors:

Hahn, Friedrich and Niesar, Aischa and Wangen, Christina and Wild, Markus and Grau, Benedikt and Herrmann, Lars and Capci, Aysun and Adrait, Annie and Couté, Yohann and Tsogoeva, Svetlana B and Marschall, Manfred

Journal:

Antiviral research (2020): 104861

A novel polythioether-based rhodamine B fluorescent probe via successive click reaction and its application in iron ion detection and cell imaging.

Authors:

Zhang, Xiaomei and Gou, Zhiming and Zuo, Yujing and Lin, Weiying

Journal:

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2020): 117679

A glutathione-responsive photosensitizer with fluorescence resonance energy transfer characteristics for imaging-guided targeting photodynamic therapy.

Authors:

Cao, Jing-Jing and Zhang, Ming-Shan and Li, Xiao-Qiang and Yang, De-Chao and Xu, Gan and Liu, Jian-Yong

Journal:

European journal of medicinal chemistry (2020): 112203

Physical properties

Molecular weight	1236.49
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.23
Correction factor (280 nm)	0.14
Extinction coefficient (cm ^-1 M ^-1)	100000 ¹
Excitation (nm)	557
Emission (nm)	570
Quantum yield	0.64 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 17, 2025