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iFluor® 555 TCO

The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. iFluor® 555 TCO can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis.
The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. iFluor® 555 TCO can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis.
Ordering information
Price ()
Catalog Number1007
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight1036.98
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.23
Correction Factor (280 nm)0.14
Extinction coefficient (cm -1 M -1)1000001
Excitation (nm)557
Emission (nm)570
Quantum yield0.641
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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iFluor® 555 succinimidyl ester
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Show More (254)

OverviewpdfSDSpdfProtocol


Molecular weight
1036.98
Correction Factor (260 nm)
0.23
Correction Factor (280 nm)
0.14
Extinction coefficient (cm -1 M -1)
1000001
Excitation (nm)
557
Emission (nm)
570
Quantum yield
0.641
The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction. iFluor® 555-TCO can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biological applications. The peptide and oligo conjugates prepared with iFluor® 555 dye are far superior to the conjugates of other spectrally similar dyes, such as the popular Cy3 and Alexa Fluor® 555. iFluor® 555 conjugates are significantly brighter and much more photostable than the corresponding conjugates of Cy3 and Alexa Fluor® 555 (Alexa Fluor® is the trademark of Invitrogen).

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 555 TCO to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM96.434 µL482.169 µL964.339 µL4.822 mL9.643 mL
5 mM19.287 µL96.434 µL192.868 µL964.339 µL1.929 mL
10 mM9.643 µL48.217 µL96.434 µL482.169 µL964.339 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (260 nm)0.23
Correction Factor (280 nm)0.14
Extinction coefficient (cm -1 M -1)1000001
Excitation (nm)557
Emission (nm)570
Quantum yield0.641

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 555 maleimide55757010000010.6410.230.14
iFluor® 555 amine55757010000010.6410.230.14
iFluor® 555 hydrazide55757010000010.6410.230.14
iFluor® 555 alkyne55757010000010.6410.230.14
iFluor® 555 azide55757010000010.6410.230.14
iFluor® 555 Styramide *Superior Replacement for Alexa Fluor 555 tyramide and Opal 570*55757010000010.6410.230.14
iFluor® 555 Tyramide55757010000010.6410.230.14
iFluor® 488 TCO4915167500010.910.210.11
iFluor® 594 TCO58860418000010.5310.050.04
iFluor® 647 TCO65667025000010.2510.030.03
iFluor® 555 Tetrazine55757010000010.6410.230.14
Show More (12)

References


View all 50 references: Citation Explorer
Novel Fluorescent Labeled Octasilsesquioxanes Nanohybrids as Potential Materials for Latent Fingerprinting Detection.
Authors: O Dare, Enock and Vendrell-Criado, Victoria and Jiménez, M Consuelo and Perez-Ruiz, Raúl and Díaz Díaz, David
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020)
Fluorescent bioorthogonal labeling of class B GPCRs in live cells.
Authors: Gangam, Srikanth Kumar and Lin, Qing
Journal: Methods in enzymology (2020): 95-111
Bond-Breaking Bio-orthogonal Chemistry Efficiently Uncages Fluorescent and Therapeutic Compounds under Physiological Conditions.
Authors: Wu, Xunshen and Wu, Kui and Gaye, Fatima and Royzen, Maksim
Journal: Organic letters (2020): 6041-6044
Fluorescent Janus emulsions for biosensing of Listeria monocytogenes.
Authors: Li, Jie and Savagatrup, Suchol and Nelson, Zachary and Yoshinaga, Kosuke and Swager, Timothy M
Journal: Proceedings of the National Academy of Sciences of the United States of America (2020): 11923-11930
Conjugable A3 adenosine receptor antagonists for the development of functionalized ligands and their use in fluorescent probes.
Authors: Federico, Stephanie and Margiotta, Enrico and Moro, Stefano and Kozma, Eszter and Gao, Zhan-Guo and Jacobson, Kenneth A and Spalluto, Giampiero
Journal: European journal of medicinal chemistry (2020): 111886
Metabolic phospholipid labeling of intact bacteria enables a fluorescence assay that detects compromised outer membranes.
Authors: Nilsson, Inga and Lee, Sheng Y and Sawyer, William S and Baxter Rath, Christopher M and Lapointe, Guillaume and Six, David A
Journal: Journal of lipid research (2020): 870-883
Optimizing the Biodistribution of Radiofluorinated Barbiturate Tracers for Matrix Metalloproteinase Imaging by Introduction of Fluorescent Dyes as Pharmacokinetic Modulators.
Authors: Schwegmann, Katrin and Hohn, Michael and Hermann, Sven and Schäfers, Michael and Riemann, Burkhard and Haufe, Günter and Wagner, Stefan and Breyholz, Hans-Jörg
Journal: Bioconjugate chemistry (2020): 1117-1132
Synthesis and Characterization of Heterodimers and Fluorescent Nisin Species by Incorporation of Methionine Analogs and Subsequent Click Chemistry.
Authors: Deng, Jingjing and Viel, Jakob H and Chen, Jingqi and Kuipers, Oscar P
Journal: ACS synthetic biology (2020)
Ultra-fast Cycling for Multiplexed Cellular Fluorescence Imaging.
Authors: Ko, Jina and Oh, Juhyun and Ahmed, Maaz S and Carlson, Jonathan C T and Weissleder, Ralph
Journal: Angewandte Chemie (International ed. in English) (2020): 6839-6846
Color-Tunable Light-up Bioorthogonal Probes for In Vivo Two-Photon Fluorescence Imaging.
Authors: Dou, Yandong and Wang, Yajun and Duan, Yukun and Liu, Bin and Hu, Qinglian and Shen, Wei and Sun, Hongyan and Zhu, Qing
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020): 4576-4582