iFluor™ 790 Azide
Ordering information
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Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1347.37 |
Solvent | Water |
Spectral properties
Correction Factor (260 nm) | 0.1 |
Correction Factor (280 nm) | 0.09 |
Extinction coefficient (cm -1 M -1) | 2500001 |
Excitation (nm) | 787 |
Emission (nm) | 812 |
Quantum yield | 0.131 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Overview | SDSProtocol |
See also: Click Chemistry
Molecular weight 1347.37 | Correction Factor (260 nm) 0.1 | Correction Factor (280 nm) 0.09 | Extinction coefficient (cm -1 M -1) 2500001 | Excitation (nm) 787 | Emission (nm) 812 | Quantum yield 0.131 |
iFluor® 790 Azide readily reacts with biomolecules that contain an alkyne group. It can be conveniently used to develop NIR imaging probers. Our iFluor® 790 dye is designed to label proteins and other biomolecules with near infrared fluorescence. Conjugates prepared with iFluor® 790 have the excitation and emission spectra similar to that of indocyanine green (ICG) and the IRDye® 800 dye, with 783/814 nm excitation/emission maxima. iFluor® 790 dye emission is well separated from commonly used far-red fluorophores such as Cy5, Cy7 or allophycocyanin (APC), facilitating multicolor analysis. This fluorophore is extremely useful for small animal in-vivo imaging applications or for other imaging applications that require NIR detections such as the two-color western applications with the LI-COR® Odyssey® infrared imaging system. In vivo fluorescence imaging uses a sensitive camera to detect fluorescence emission from fluorophores in whole-body living small animals. To overcome the photon attenuation in living tissue, fluorophores with long emission at the near-infrared (NIR) region are generally preferred, including widely used small indocarbocyanine dyes. Recent advances in imaging strategies and reporter techniques for in vivo fluorescence imaging include novel approaches to improve the specificity and affinity of the probes and to modulate and amplify the signal at target sites for enhanced sensitivity. Further emerging developments are aiming to achieve high-resolution, multimodality and lifetime-based in vivo fluorescence imaging.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of iFluor® 790 Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 74.219 µL | 371.093 µL | 742.187 µL | 3.711 mL | 7.422 mL |
5 mM | 14.844 µL | 74.219 µL | 148.437 µL | 742.187 µL | 1.484 mL |
10 mM | 7.422 µL | 37.109 µL | 74.219 µL | 371.093 µL | 742.187 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (260 nm) | 0.1 |
Correction Factor (280 nm) | 0.09 |
Extinction coefficient (cm -1 M -1) | 2500001 |
Excitation (nm) | 787 |
Emission (nm) | 812 |
Quantum yield | 0.131 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 647 azide | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
iFluor® 488 azide | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 555 azide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 790 Styramide *Superior Replacement for Alexa Fluor 790 tyramide* | 787 | 812 | 2500001 | 0.131 | 0.1 | 0.09 |
iFluor™ 405 azide | 403 | 427 | 370001 | 0.911 | 0.48 | 0.77 |
Images
Figure 1. iFluor® 790 Azide readily reacts with biomolecules that contain an alkyne group. It can be conveniently used to develop NIR imaging probers. Our iFluor™ 790 dye is designed to label proteins and other biomolecules with near infrared fluorescence. Conjugates prepared with iFluor™ 790 have the excitation and emission spectra similar to that of indocyanine green (ICG) and the IRDye® 800 dye, with 783/814 nm excitation/emission maxima.
References
View all 19 references: Citation Explorer
Impact of Surgical Lights on the Performance of Fluorescence-Guided Surgery Systems: A Pilot Study.
Authors: Chiti, Lavinia E and Park, Brian and d'Orchymont, Faustine and Holland, Jason P and Nolff, Mirja C
Journal: Animals : an open access journal from MDPI (2023)
Authors: Chiti, Lavinia E and Park, Brian and d'Orchymont, Faustine and Holland, Jason P and Nolff, Mirja C
Journal: Animals : an open access journal from MDPI (2023)
The Dual-Targeted Peptide Conjugated Probe for Depicting Residual Nasopharyngeal Carcinoma and Guiding Surgery.
Authors: Huang, Wenhui and He, Zicong and Cai, Xuekang and Zhang, Jingming and Li, Wei and Wang, Kun and Zhang, Shuixing
Journal: Biosensors (2022)
Authors: Huang, Wenhui and He, Zicong and Cai, Xuekang and Zhang, Jingming and Li, Wei and Wang, Kun and Zhang, Shuixing
Journal: Biosensors (2022)
Deep learning for in vivo near-infrared imaging.
Authors: Ma, Zhuoran and Wang, Feifei and Wang, Weizhi and Zhong, Yeteng and Dai, Hongjie
Journal: Proceedings of the National Academy of Sciences of the United States of America (2021)
Authors: Ma, Zhuoran and Wang, Feifei and Wang, Weizhi and Zhong, Yeteng and Dai, Hongjie
Journal: Proceedings of the National Academy of Sciences of the United States of America (2021)
Measuring Kidney Perfusion, pH, and Renal Clearance Consecutively Using MRI and Multispectral Optoacoustic Tomography.
Authors: Minhas, Atul S and Sharkey, Jack and Randtke, Edward A and Murray, Patricia and Wilm, Bettina and Pagel, Mark D and Poptani, Harish
Journal: Molecular imaging and biology (2020): 494-503
Authors: Minhas, Atul S and Sharkey, Jack and Randtke, Edward A and Murray, Patricia and Wilm, Bettina and Pagel, Mark D and Poptani, Harish
Journal: Molecular imaging and biology (2020): 494-503
Fast DNA Extraction with Polyacrylamide Microspheres for Polymerase Chain Reaction Detection.
Authors: Wang, Jun and Zhang, Shuyan and Xu, Xinhui and Xing, Yujun and Li, Zongru and Wang, Jinke
Journal: ACS omega (2020): 13829-13839
Authors: Wang, Jun and Zhang, Shuyan and Xu, Xinhui and Xing, Yujun and Li, Zongru and Wang, Jinke
Journal: ACS omega (2020): 13829-13839
Evaluation of Targeting Efficiency of Joints with Anticollagen II Antibodies.
Authors: Lofchy, Laren A and Vu, Vivian P and Banda, Nirmal K and Ramirez, Joseline Ramos and Smith, Weston J and Gifford, Geoffrey and Gaikwad, Hanmant and Scheinman, Robert I and Simberg, Dmitri
Journal: Molecular pharmaceutics (2019): 2445-2451
Authors: Lofchy, Laren A and Vu, Vivian P and Banda, Nirmal K and Ramirez, Joseline Ramos and Smith, Weston J and Gifford, Geoffrey and Gaikwad, Hanmant and Scheinman, Robert I and Simberg, Dmitri
Journal: Molecular pharmaceutics (2019): 2445-2451
Near-infrared fluorescence laparoscopy of the ureter with three preclinical dyes in a pig model.
Authors: van den Bos, Jacqueline and Al-Taher, Mahdi and Bouvy, Nicole D and Stassen, Laurents P S
Journal: Surgical endoscopy (2019): 986-991
Authors: van den Bos, Jacqueline and Al-Taher, Mahdi and Bouvy, Nicole D and Stassen, Laurents P S
Journal: Surgical endoscopy (2019): 986-991
Evaluation of a novel dye for near-infrared fluorescence delineation of the ureters during laparoscopy.
Authors: Al-Taher, M and van den Bos, J and Schols, R M and Kubat, B and Bouvy, N D and Stassen, L P S
Journal: BJS open (2018): 254-261
Authors: Al-Taher, M and van den Bos, J and Schols, R M and Kubat, B and Bouvy, N D and Stassen, L P S
Journal: BJS open (2018): 254-261
A Modular Dual-Labeling Scaffold That Retains Agonistic Properties for Somatostatin Receptor Targeting.
Authors: Ghosh, Sukhen C and Rodriguez, Melissa and Carmon, Kendra S and Voss, Julie and Wilganowski, Nathaniel L and Schonbrunn, Agnes and Azhdarinia, Ali
Journal: Journal of nuclear medicine : official publication, Society of Nuclear Medicine (2017): 1858-1864
Authors: Ghosh, Sukhen C and Rodriguez, Melissa and Carmon, Kendra S and Voss, Julie and Wilganowski, Nathaniel L and Schonbrunn, Agnes and Azhdarinia, Ali
Journal: Journal of nuclear medicine : official publication, Society of Nuclear Medicine (2017): 1858-1864
Longitudinal monitoring of skin accumulation of nanocarriers and biologicals with fiber optic near infrared fluorescence spectroscopy (FONIRS).
Authors: Griffin, James I and Benchimol, Michael J and Simberg, Dmitri
Journal: Journal of controlled release : official journal of the Controlled Release Society (2017): 167-174
Authors: Griffin, James I and Benchimol, Michael J and Simberg, Dmitri
Journal: Journal of controlled release : official journal of the Controlled Release Society (2017): 167-174