zFluor™ 647 succinimidyl ester

Our zFluor™ serial dyes are developed to have the possibly highest photostability at a given wavelength compared to the other similar wavelength dyes on the market. zFluor™ 647 has almost identical spectral properties to the popular Cy5® (GE Healthcare) and Alexa Fluor® 647 (ThermoFisher). Its high thermal and photostability makes it an excellent choice for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM and STED. Under the same test conditions, zFluor™ 647 has much higher ozone stability than Cy5® and Alexa Fluor® 647, making it a much better choice for microarray and other biochip-based applications. In addition, zFluor™-labeled oligonucleotides and peptides are much brighter and more photostable than the ones labeled by Alexa Fluor® 647 and Cy5®. This feature is extremely useful for fluorescence in-situ hybridization (FISH). In common with our iFluor® labels, the absorption and fluorescence of our zFluor™ 647 are independent of pH in the range of pH 2 to 11. It is well excited at 633 nm of He-Ne laser, the 647 nm line of the Krypton-Ion laser or a diode-laser emitting at 650 nm.

<p>Photostability of zFluor 647™ in comparison with Cy5. Equimolar concentrations of the dyes were continuously illuminated and fluorescence was measure every 5 seconds. Fluorescence values were normalized to the initial intensity.</p>

Protocol

SDS

COA

Catalog	Size	Price	Quantity
1500	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of zFluor™ 647 succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	117.228 µL	586.139 µL	1.172 mL	5.861 mL	11.723 mL
5 mM	23.446 µL	117.228 µL	234.456 µL	1.172 mL	2.345 mL
10 mM	11.723 µL	58.614 µL	117.228 µL	586.139 µL	1.172 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	853.04 g/mol	=	mL	x	mM	=	mmol

References

View all 19 references: Citation Explorer

Single nucleotide polymorphism discrimination with and without an ethidium bromide intercalator

Authors:

Fenati, R. A.; Connolly, A. R.; Ellis, A. V.

Journal:

Anal Chim Acta (2017): 121-128

Generation and Characterization of Virus-Enhancing Peptide Nanofibrils Functionalized with Fluorescent Labels

Authors:

Rode, S.; Hayn, M.; Rocker, A.; Sieste, S.; Lamla, M.; Markx, D.; Meier, C.; Kirchhoff, F.; Walther, P.; F and rich, M.; Weil, T.; Munch, J.

Journal:

Bioconjug Chem (2017): 1260-1270

Capillary-Driven Microfluidic Chips for Miniaturized Immunoassays: Patterning Capture Antibodies Using Microcontact Printing and Dry-Film Resists

Authors:

Temiz, Y.; Lovchik, R. D.; Delamarche, E.

Journal:

Methods Mol Biol (2017): 37-47

Background Suppression in Imaging Gold Nanorods through Detection of Anti-Stokes Emission

Authors:

Carattino, A.; Keizer, V. I.; Schaaf, M. J.; Orrit, M.

Journal:

Biophys J (2016): 2492-2499

Absolute two-photon excitation spectra of red and far-red fluorescent probes

Authors:

Velasco, M. G.; Allgeyer, E. S.; Yuan, P.; Grutzendler, J.; Bewersdorf, J.

Journal:

Opt Lett (2015): 4915-8

Physical properties

Molecular weight	853.04
Solvent	DMSO

Spectral properties

Excitation (nm)	648
Emission (nm)	668

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 13, 2025