iFluor® 680 succinimidyl ester

iFluor® 680 succinimidyl ester is an amine-reactive, far-red fluorescent dye designed for covalent labeling of proteins, particularly antibodies, for fluorescence detection applications.

Amine-Reactive Labeling: Exhibits good reactivity and selectivity toward protein amino groups.
Spectral Properties: Excitation/emission maxima of 684/701 nm.
Photostability: Provides bright, photostable fluorescence with minimal protein quenching.
Dye Compatibility: Spectrally similar to Cy5.5® and Alexa Fluor® 680.

<p>HeLa cells were stained with mouse anti-tubulin followed with iFluor<sup>TM</sup> 680 goat anti-mouse IgG (H+L); and nuclei were stained with Nuclear Green<sup>TM</sup> DCS1 (Cat# 17550).</p>

Protocol

Catalog	Size	Price
1035	1 mg	Price
71035	100 ug	Price
71513	5 mg	Price
71563	10 mg	Price

Overview

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 680 dyes have fluorescence excitation and emission maxima of ~684 nm and ~701 nm respectively. These spectral characteristics make them an excellent alternative to Cy5.5® and Alexa Fluor® 680 labeling dyes (Cy5.5® and Alexa Fluor® are the trademarks of GE Health Care and Invitrogen). iFluor® 680 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 680 succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	104.475 µL	522.373 µL	1.045 mL	5.224 mL	10.447 mL
5 mM	20.895 µL	104.475 µL	208.949 µL	1.045 mL	2.089 mL
10 mM	10.447 µL	52.237 µL	104.475 µL	522.373 µL	1.045 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	957.17 g/mol	=	mL	x	mM	=	mmol

Citations

View all 3 citations: Citation Explorer

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification

Authors:

Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others

Journal:

Metabolomics (Los Angel) (2017): 2153--0769

Suramin inhibits cullin-RING E3 ubiquitin ligases

Authors:

Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others

Journal:

Proceedings of the National Academy of Sciences (2016): E2011--E2018

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function

Authors:

Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra and Martens, Erik and Liekens, S and ra and Noppen, Sam and Damme, Jo Van and others

Journal:

International Journal of Cancer (2012): E425--E436

References

View all 49 references: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes

Authors:

Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.

Journal:

Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling

Authors:

Zhang S, Tan HC, Ooi EE.

Journal:

J Vis Exp. (2011)

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells

Authors:

Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.

Journal:

Biochem Biophys Res Commun (2011): 211

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Authors:

Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.

Journal:

Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers

Authors:

Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.

Journal:

J Microbiol Methods (2011): 119

Physical properties

Molecular weight	957.17
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.097
Correction factor (280 nm)	0.094
Extinction coefficient (cm ^-1 M ^-1)	220000 ¹
Excitation (nm)	684
Emission (nm)	701
Quantum yield	0.23 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on November 6, 2026