iFluor® 700 succinimidyl ester

iFluor® 700 succinimidyl ester is an amine-reactive, far-red fluorescent dye designed for covalent labeling of proteins, particularly antibodies, for fluorescence detection applications.

Amine-Reactive Labeling: Exhibits good reactivity and selectivity toward protein amino groups.
Spectral Properties: Excitation/emission maxima of 690/713 nm.
Photostability: Provides bright, photostable fluorescence with minimal protein quenching.
Dye Compatibility: Spectrally similar to Alexa Fluor® 700.

HeLa cells were incubated with (Tubulin+) or without (Tubulin-) mouse anti-tubulin followed by iFluor® 700 goat anti-mouse IgG conjugate (Left) or Alexa Fluor® 700 goat anti-mouse IgG conjugate (Right), respectively. Alexa Fluor® 700 goat anti-mouse IgG conjugates gave non-specific nuclear staining.

Protocol

Catalog	Size	Price
1036	1 mg	Price
71036	100 ug	Price
71514	5 mg	Price
71564	10 mg	Price

Overview

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 700 dyes have fluorescence excitation and emission maxima of ~690 nm and ~713 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 700 labeling dye (Alexa Fluor® is the trademark of Invitrogen). iFluor® 700 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 700 succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	102.337 µL	511.687 µL	1.023 mL	5.117 mL	10.234 mL
5 mM	20.467 µL	102.337 µL	204.675 µL	1.023 mL	2.047 mL
10 mM	10.234 µL	51.169 µL	102.337 µL	511.687 µL	1.023 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	977.16 g/mol	=	mL	x	mM	=	mmol

Citations

View all 3 citations: Citation Explorer

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification

Authors:

Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined

Journal:

Metabolomics (Los Angel) (2017): 2153--0769

Suramin inhibits cullin-RING E3 ubiquitin ligases

Authors:

Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined

Journal:

Proceedings of the National Academy of Sciences (2016): E2011--E2018

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function

Authors:

Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined

Journal:

International Journal of Cancer (2012): E425--E436

References

View all 49 references: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes

Authors:

Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.

Journal:

Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling

Authors:

Zhang S, Tan HC, Ooi EE.

Journal:

J Vis Exp. (2011)

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells

Authors:

Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.

Journal:

Biochem Biophys Res Commun (2011): 211

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Authors:

Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.

Journal:

Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers

Authors:

Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.

Journal:

J Microbiol Methods (2011): 119

Physical properties

Molecular weight	977.16
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.09
Correction factor (280 nm)	0.04
Correction factor (565 nm)	0.0766
Correction factor (650 nm)	0.4004
Extinction coefficient (cm ^-1 M ^-1)	220000 ¹
Excitation (nm)	690
Emission (nm)	713
Quantum yield	0.23 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Fluorescence microplate reader
Excitation	691 nm
Emission	711 nm
Cutoff	700 nm
Recommended plate	Solid black

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Page updated on October 28, 2026