iFluor® 820 succinimidyl ester

iFluor® 820 succinimidyl ester is an amine-reactive, near-infrared fluorescent dye designed for covalent labeling of proteins and other biomolecules for fluorescence imaging and detection applications.

Amine-Reactive Labeling: Covalently conjugates to primary amino groups of proteins.
Spectral Properties: Excitation/emission maxima of 822/850 nm.
Spectral Compatibility: Infrared emission is well separated from Cy5, Cy7, and APC fluorophores.
Applications: Suitable for near-infrared small-animal imaging and multicolor fluorescence analysis.

<p>Fluorescent dye NHS esters (or succinimidyl esters) are the most popular tool for conjugating dyes to a peptide, protein, antibody, amino-modified oligonucleotide or nucleic acid. NHS esters react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.</p>

Protocol

Catalog	Size	Price
1399	1 mg	Price
71399	100 ug	Price
71527	5 mg	Price
71577	10 mg	Price

Overview

In vivo fluorescence imaging uses a sensitive camera to detect the fluorescence emission from fluorophores in whole-body living small animals. To overcome the photon attenuation in living tissue, fluorophores with long emission at the infrared (IR) region are generally preferred. Recent advances in imaging strategies and reporter techniques for in vivo fluorescence imaging include novel approaches to improve the specificity and affinity of the probes and to modulate and amplify the signal at target sites for enhanced sensitivity. Further emerging developments aim to achieve high-resolution, multimodality, and lifetime-based in vivo fluorescence imaging. Our iFluor® 820 is designed to label proteins and other biomolecules with infrared fluorescence. Conjugates prepared with iFluor® 820 have excitation and emission in the IR range. iFluor® 820 dye emission is well separated from commonly used far-red fluorophores such as Cy5, Cy7, or allophycocyanin (APC), facilitating multicolor analysis. This fluorophore is also useful for small animal in vivo imaging applications or other imaging applications requiring IR detections. iFluor® 820 succinimidyl ester is amine-reactive and can be readily used to conjugate amine-containing biomolecules, particularly antibodies.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 820 succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	63.055 µL	315.274 µL	630.549 µL	3.153 mL	6.305 mL
5 mM	12.611 µL	63.055 µL	126.11 µL	630.549 µL	1.261 mL
10 mM	6.305 µL	31.527 µL	63.055 µL	315.274 µL	630.549 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1585.92 g/mol	=	mL	x	mM	=	mmol

References

View all 18 references: Citation Explorer

A target cell-specific activatable fluorescence probe for in vivo molecular imaging of cancer based on a self-quenched avidin-rhodamine conjugate

Authors:

Hama Y, Urano Y, Koyama Y, Kamiya M, Bernardo M, Paik RS, Shin IS, Paik CH, Choyke PL, Kobayashi H.

Journal:

Cancer Res (2007): 2791

Fluorescence imaging in vivo: recent advances

Authors:

Rao J, Dragulescu-Andrasi A, Yao H.

Journal:

Curr Opin Biotechnol (2007): 17

Ex vivo fluorescence imaging of normal and malignant urothelial cells to enhance early diagnosis

Authors:

Steenkeste K, Lecart S, Deniset A, Pernot P, Eschwege P, Ferlicot S, Leveque-Fort S, Bri and et R, Fontaine-Aupart MP.

Journal:

Photochem Photobiol (2007): 1157

In vivo monitoring the fate of Cy5.5-Tat labeled T lymphocytes by quantitative near-infrared fluorescence imaging during acute brain inflammation in a rat model of experimental autoimmune encephalomyelitis

Authors:

Berger C, Gremlich HU, Schmidt P, Cannet C, Kneuer R, Hiest and P, Rausch M, Rudin M.

Journal:

J Immunol Methods (2007): 65

A protocol for imaging alternative splicing regulation in vivo using fluorescence reporters in transgenic mice

Authors:

Bonano VI, Oltean S, Garcia-Blanco MA.

Journal:

Nat Protoc (2007): 2166

Physical properties

Molecular weight	1585.92
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.11
Correction factor (280 nm)	0.16
Extinction coefficient (cm ^-1 M ^-1)	250000 ¹
Excitation (nm)	822
Emission (nm)	850

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 29, 2026